有eastern blot这项技术么?和其它三种有什么区别么

daringnana1582022-10-04 11:39:541条回答

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天空yy 共回答了14个问题 | 采纳率78.6%
eastern blot:电驱动转移等电聚焦电泳后的蛋白区带
biotting历史如下(来自电泳的原理应用及进展):
转印电泳最先是由Southern于1975年发明的.在电流作用下,他成功地将DNA片段从琼脂糖凝胶中转印到硝酸纤维膜上进行分子杂交分析,因此称为Southern-blotting.后来,Alwine用类似方法也成功地将RNA从电泳胶中转印到硝酸纤维膜上作分子杂交分析,但他并没有称这一技术为Alwine-blotting,而是称之为Northern-blotting,以便与Sourhern blotting相对应.1981年Burette又成功地将SDS-PAGE胶中的蛋白质转印到膜上进行免疫学分析(如抗原抗体结合、蛋白质与配基结合等),继之Alwine,Burette称这一技术为Western-blotting.这样一来,在转印电泳这个家族中,就有了Southernblotting,Northern-blotting和Western-blotting,仅仅缺一个Eastern-blotting.其实后来有人提议将IEF胶(即等电聚焦电泳)中的蛋白质转印到膜上的技术称为Eastern-blotting,但这一建议并未被广泛接受
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英语翻译New Zealand is an island that lies off the eastern coast
英语翻译
New Zealand is an island that lies off the eastern coast of Australia.It is made up of two large islands :North Island and South Island .New Zealand is surrounded by the Pacific Ocean to the north and east ,and the Tasman Sea to the south and west .It is about the same size as the Philippines .Wellington ,the capital city ,lies on the North Island .Other important cities are Auckland in the north ,Christchurch on the South Island and Queenstown ,further to the south .
New Zealand has a mild sea climate ,while the north is subtropical .It rains quite a lot .The warmest months are December to February .The coldest months are June to August .
The ocean and seas surrounding the islands are deep blue and many of New Zealand’s cities lie on a bay and have a natural deep harbour .The sand beaches in New Zealand are also very clean .New Zealand has a very beautiful natural landscape with green hills and mountains .Some of the mountains are dead volcanoes .The North Island is famous for an area of hot springs ,some of which throw hot water high into the air .Some of this heat near the earth’s surface is used to make electricity .There are many plants and animals that only live in New Zealand.The most famous animal is a little bird that cannot fly .It is called a kiwi and it is New Zealand’s national bird .
The Maori were the earliest people to come to New Zealand .When they discovered it about 1000 years ago ,they called it Aotearoa ,which means “Land of the long white cloud”.The Maori came from the islands of Polynesia in the Pacific .It is some 3500 kilometres from Polynesia to New Zealand ,which they traveled in narrow boats .They brought dogs ,rats and plants with them and settled mainly on the North Island where the weather was warmer .Around 1421 ,Chinese sailors discovered the islands on one of their voyages around the world .The Dutchman Abel Tasman named the islands New Zealand in 1642 .More than 120 years later ,Captain James Cook took possession of the islands in 1769 and from that time British people started to settle in New Zealand .By 1840 about 2000 Europeans ,mainly British ,had come to settle in New Zealand ant the Maori signed an agreement with these settlers .That day is still celebrated as a national holiday .It is every year on the sixth of February .Today there are still many people in New Zealand who call England “ the home country “ ,although it is a place where most of them have never been .
认认真真的活1年前2
liuxu525 共回答了18个问题 | 采纳率88.9%
新西兰是一个岛国,位于东海岸的澳大利亚.它由两个大岛:北岛和南岛.新西兰周围是太平洋的北部和东部,和塔斯曼海到南部和西部.这是有关的尺寸相同,菲律宾.惠灵顿首都,位于北岛.其他重要城市是奥克兰北部,克赖斯特彻奇...
Nanjing.China.in.is.Jiangsu.in.Province.eastern(连词成句)
小楼风雨1年前2
在虚无缥缈 共回答了19个问题 | 采纳率73.7%
Nanjing is eastern in Jiangsu Province in China
谁来帮忙做一下这篇英语阅读?On the eastern part of West Malaysia,there is
谁来帮忙做一下这篇英语阅读?
on the eastern part of west malaysia,there is a long stretch(平坦的一片) of sandy beach.from the month of june to august every year,giant turtles(大海龟) come ashore(向岸上) to lay eggs.they appear only late at night to lay their eggs in holes which they dig with their hind flippers(鳍状肢).
when they have finished laying their eggs,they fill up the holes with sand and return to the sea.the mother turtles never see their babies.the hot sun keeps the eggs warm until they hatch(孵化).once hatched,the baby turtles will crawl back to the sea to begin their new lives.in the past,many people came to dig up the eggs after the mother turtles had left.they brought the eggs to town to sell.
since 1962,the malaysian *** has built high fences(篱笆) around the nurseries(育卵场) to control the digging and sale of eggs for fear that the giant turtles may become extinct(灭绝).
根据短文内容选择最佳答案.(答案涂在答题纸上)
21.the giant turtles lay their eggs______.
a.in the sea b.twice a year
c.in the morning d.for a period of three months
22.the giant turtles hide their eggs by______.
a.sitting on them b.covering them with sand
c.keeping them in the shade(阴凉处)
d.digging holes with their flippers
23.after laying the eggs,the mother turtles______.
a.return to the sea b.live on the beach
c.leave after they have seen their babies
d.keep the eggs warm until they hatched
24.some people dug up the eggs because they wanted to______.
a.make money b.keep turtles as pets
c.give them as presents
d.prevent the turtles from becoming extinct
25.the high fences are built to______the eggs.
a.cook b.hatch c.protect d.sell
到底哪个啊
mumin1980iii1年前3
小梅飘雪 共回答了20个问题 | 采纳率100%
DDAAC
by 20:00 or 10:00 p.m. Eastern Time in the United States on
by 20:00 or 10:00 p.m. Eastern Time in the United States on the due dates.有人知道这是什么意思吗?
我知道这句话意思.我想问是不是美国20:00和10:00pm是一个东西?
这是一个很重要的文件里面的.按道理来说 这儿应该只会出现一个时间.
在我的常识里,20:00指的是8:00pm.
但是文件里的答复截止时间 的确是20:00 or 10:00pm.
有人知道这是怎么回事吗?
rerwrwr1年前1
wlyjt 共回答了19个问题 | 采纳率94.7%
美国有六个时区
每个时区对应一个标准时间,从东向西分别为东部时间(EST)(西五区时间)、中部时间(CST)(西六区时间)、山地时间(MST)(西七区时间)、太平洋时间(西部时间)(PST)(西八区时间)、阿拉斯加时间(AKST)(西九区时间)和夏威夷时间(HST)(西十区时间),按照“东早西晚”的规律,各递减一小时
所以根据这个 楼主文件所说的第一个时间就是山地时间,20:00 这时也就是东部时间的22:00了
eastern和east的区别in the ____(east) part of China 填什么
行冉1年前1
junil 共回答了15个问题 | 采纳率100%
空里填eastern ,因为part是名词需要形容词修饰,east 是名词,eastern 是形容词.
east和eastern的区别是什么?这两个单词怎么用?
山月胧明1年前1
小瓶可乐 共回答了18个问题 | 采纳率94.4%
east可作名词,东方,东部,China is in the east of Asia.
形容词,东方的,东部的,朝东的,the side of the street.
副词,在东方,向东方,The room faces east.
eastern可作形容词,东方的,东部的
名词,东方人
英语翻译The flood ,they say,triggered the sinking of Eastern Pha
英语翻译
The flood ,they say,triggered the sinking of Eastern Pharaohs were coming to an end ,the cities' flourished as gateways to Egypt.
jianayue1年前4
kpxu 共回答了30个问题 | 采纳率86.7%
The flood 主语,they say 插入语,triggered the sinking of Eastern Pharaohs后置定语were coming to an end.
据说,要使东面的法老沉下去的洪水结束了,作为通向埃及的通道的城市繁荣了起来.
英语翻译A pipeline will be built to () some eastern provinces wi
英语翻译
A pipeline will be built to () some eastern provinces with natural gas from the west of China.
A supply
B send
C sell
D offer
为什么选它?
mayatime1年前1
giselafee 共回答了16个问题 | 采纳率93.8%
A supply
原因:
1,句意:给东部各省【供应】天然气
2,语法:supply做“供应”讲时,后面不能接双宾语,要用supply sb.with sth.加个with
How to narrow the differences between eastern and western cu
How to narrow the differences between eastern and western cultures?这个问题用英语怎么回答?
不能太短呀~怎么也得几句话吧?
zp3321年前3
wupei_2005 共回答了18个问题 | 采纳率100%
你的问题是:怎样缩小东西文化的差异?
我的回答是:相互沟通,相互学习,相互融入.
Communicate with each other,learn from each other and Into each other .
写问句 The bookstore is in the eastern of the cinema.&&&
mmqm0151年前3
neilsydney 共回答了18个问题 | 采纳率88.9%
Where is the bookstore?
Eastern Caribbean Central Bank是什么意思及反义词
沫沫01年前2
miao_kiss 共回答了20个问题 | 采纳率95%
Eastern Caribbean Central Bank
东加勒比中央银行
双语对照
词典结果:
网络释义
1.东加勒比中央银行
2.东加勒比海中央银行
例句:
1.
Agreement establishing the caribbean development bank;
建立加勒比开发银行的协定;
2.
Belizeans consider themselves to culturally be both caribbean and centralamerican.
贝里斯人认为他们兼具加勒比海和中美洲文化.
英语翻译LocationLocated on the eastern side of George Street,bet
英语翻译
Location
Located on the eastern side of George Street,between Alfred
and Dalley Streets,Lippo House is within a short walk to
Circular Quay and Wynyard bus and rail interchange.
Building Description
A boutique 18 level B-Grade building completed in 1990.The
building comprises 1 level of retail shopping and 15 upper floors
overlooking George Street.
Property Characteristics
• Year Refurbished - 1997
• Building Grade - B
• Lease - RealCommercial ID - 5470477
• Total
huhuhalu1年前3
qlk12 共回答了23个问题 | 采纳率78.3%
Location (地点)
Located on the eastern side of George Street,between Alfredand Dalley Streets,Lippo House is within a short walk toCircular Quay and Wynyard bus and rail interchange(位于乔治街东侧,与艾尔弗雷德和达利街道,力宝大厦,是在短期内步行到,环形码头和温亚德公共汽车和铁路交汇处)
Building Description(建筑描述)
A boutique 18 level B-Grade building completed in 1990.(精品18级B级大楼落成于1990年)
Thebuilding comprises 1 level of retail shopping and 15 upper floorsoverlooking George Street.(该建筑物包括1级的零售商场和15个楼层俯瞰乔治街.)
Property Characteristics(性能特点)
• Year Refurbished - 1997(•年份翻新- 1997)
• Building Grade - B(建筑档次-乙)
• Lease - RealCommercial ID - 5470477(租赁-RealCommercial号 -5470477)
• Total(总面额)
美国eastern standard time的上午9点是北京时间几点?
zl4261年前1
我是老颜 共回答了17个问题 | 采纳率100%
次日上午10 点
Neil Williams ,a PE teacher at Eastern Connecticut State Uni
Neil Williams ,a PE teacher at Eastern Connecticut State University ,even made a PE Hall of Shame
in which dodgeball is included.
额……麻烦,帮我翻译成中文……
lucklay1年前1
caofen0828 共回答了24个问题 | 采纳率87.5%
尼尔 威廉姆斯,东康涅狄格州立大学的一位体育老师,甚至做了一本体育耻辱簿,里面包括了躲避球(这一项目).
Nearly all of the children have been to Eastern Europe.怎么翻译
cherishtangrh1年前1
长乐猫王 共回答了15个问题 | 采纳率100%
这些孩子几乎都已经去东欧了.
the risIng sun of (f__) the eastern sky(首字母天空)
the risIng sun of (f__) the eastern sky(首字母天空)
the risIng sun (f__) the eastern sky(首字母填空) 太对不起大家了,我的题目出错了.
天堂宝宝猪1年前1
niesj 共回答了17个问题 | 采纳率76.5%
flames ,朝阳染红了东方的天空.
flame在这做动词用,意思是“照亮”,其名词意思是"火焰"
There are many differences between Eastern and Western ____(
There are many differences between Eastern and Western ____(culture).
macro_21年前1
105223844 共回答了19个问题 | 采纳率84.2%
cultural 文化.句中意思是东方文化与西方文化有很多不同点. 有帮助的话望LZ采纳!
关于“逆时针”的英文翻译?Eastern字面意思是东方的,为什么也可以翻译为逆时针,逆时针怎么翻译更好更形象,能体现出时
关于“逆时针”的英文翻译?
Eastern字面意思是东方的,为什么也可以翻译为逆时针,逆时针怎么翻译更好更形象,能体现出时光倒流之意?
金营1年前1
cathyviolin 共回答了24个问题 | 采纳率79.2%
逆时针也可以说是 counterclockwise,这是逆时针的意思。
如果要说时光倒流的话Back in time 会更好一点。
望采纳~
如果还有问题请追问~