英语翻译The columns were washed with 3.0mL water and thenwith 3.

英语翻译
The columns were washed with 3.0mL water and then
with 3.0mL of a methanol:0.02 mol/L pH 6.5 phosphate buffer
(50:50,v/v),followed by a vacuum of 2min prior to elution of
the analytes with 3.0mL methanol containing 10% acetic acid.
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