英语翻译Assignment of whether protein pairs were orthologous,hom

Assignment of whether protein pairs were orthologous, homologous, paralogous, or unrelated was assessed manually on an individual basis. Data and evidence supporting protein similarity are provided in Supplementary Table 1.
对蛋白偶对是否为直系同源、异体同源、旁系同源或无关联这项任务分配进行了基于个体的人工评估.支持蛋白质相似性的数据和证据见补充表1.
EdU/BrdU double labeling. Parasites labeled with 10 mM EdU and BrdU were fixed in Methacarn (6:3:1 methanol: chloroform: glacial acetic acid) or processed for in situ hybridization. After a 45-min 2N HCl treatment, EdU was detected and parasites were processed for anti-BrdU immunofluorescence (anti-BrdU 1:500, clone MoBU, Invitrogen). We observed no cross reactivity between this antibody and EdU.
EdU/BrdU双标记.以10mM EdU和BrdU标记的寄生虫固定到麦撒卡恩(methacarn)（6:3:1甲醇：氯仿：冰醋酸）中,或进行现场杂交.经过45分钟的2N赖氨酸处理后,检测到EdU,并对寄生虫进行抗BrdU免疫荧光（抗BrdU 1:500,克隆MoBU,Invitrogen公司）处理.我们观察到这种抗体和EdU之间无交叉反应.
To quantify the level of BrdU/EdU overlap and measure centre-to-centre distances between nuclei, three-dimensional confocal stacks from EdU/BrdU labeled animals were re-sampled to give isotropic voxels, and subjected to Gaussian filtering and background-subtraction. Labeled nuclei were segmented with Imaris (Bitplane Inc.) using parameters empirically determined to minimize the need for manual corrections; typically, fewer than 5% of the total nuclei required correction. The three-dimensional coordinates of the nuclei were exported and analysed with MATLAB. Overlapping EdU- and BrdU-labeled nuclei were defined as nuclei with centre-to-centre distances, 1 nuclear size (, 4 mm). Statistical analyses were performed in Origin (OriginLab).
为了对BrdU/EdU重叠的水平进行量化并测量原子核之间中心到中心的距离,从EdU/BrdU标记的动物中进行了三维共焦架重新采样,以给出各向同性三维像素,并经过高斯滤波和背景减除.标记的细胞核通过经验确定的参数用（Bitplane公司的）伊万里瓷器(imaris) 分割以尽量减少人工校正的需要；通常情况下,只有不到5%的总细胞核需要校正.用MATLAB输出和分析三维坐标细胞核.将EdU和BrdU标记的细胞核的重叠部分即细胞核中心到中心的距离定义为1个细胞核尺寸（,4毫米）.用（Origin实验室的）Origin进行统计分析.