英语翻译Through the use of a metal ion affinity column,phospho-r

英语翻译
Through the use of a metal ion affinity column,phospho-
rylated peptides can be enriched for phosphorylation site
mapping at the proteome level [21].For example,this
strategy was utilized to identify unique phosphorylation
sites from elicitor-treated Arabidopsis plants [22].Many
groups have also utilized membrane shaving followed by
metal ion affinity chromatography and MS analysis to
identify phosphorylation sites [15,23-26].In 2008,the
Santoni group utilized MS and identified novel phospho-
rylation sites in Arabidopsis aquaporin PIP2;1 following
salt stress [27].However,the phosphorylation sites of
other membrane proteins under the abiotic stress condi-
tion are still not well characterized.