A biochemist wants to separate two peptides by ionexchange c

machel_112022-10-04 11:39:542条回答

A biochemist wants to separate two peptides by ionexchange chromatography.At the pH of the mobile phase to be used on the column,one peptide (A) has a net charge of 53,due to the presence of more Glu and Asp residues than Arg,Lys,and His residues.Peptide B has a net charge of 31.Which peptide would elute first from a cation-exchange resin?Which would elute first from an anion-exchange resin?

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一切由自己 共回答了22个问题 | 采纳率86.4%
大概意思是某生物学家想用离子交换色谱分离一个肽,在中性条件下过柱,A的净电荷为3,因为Glu 和 Asp残留物比Arg,Lys,多,b肽净电荷1,问那个先从阳离子交换树脂洗脱下来,那个先从阴离子交换树脂洗脱下来,
1年前
好路 共回答了88个问题 | 采纳率
一个生物化学家想单独的两个肽通过离子置换色谱法。在流动相的pH值是用在列,一个肽(一)有一个网费3,由于存在更多的谷胺酸和Asp残留fuel赖氨酸,比他的残留物。肽B有净电荷为1。这将首先从一个肽洗提阳离子交换树脂?这将首先从一个阴离子交换树脂洗提?...
1年前

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