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歌曲名:Welcome Back歌手:松隆子专辑:Harvest SongsYoung Jeezy - Welcome BackYeah I told you NiggasYeah NiggasI told yalls I was coming back niggasI want it down below homiesLets goWelcome backGuess whos back and he is shinin on you niggasWelcome backGuess whos back and he is stuntin on you hoesWelcome backYea I"m back and I"m shinin on you niggasWelcome backYea I"m back and I"m stuntin on these hoesBeen on my grizzy but now I"m back in the placeAnd if you show me the money ill put this brick in your faceAnd if ya aint got mo money ill put this strap in your faceAnd believing these niggas is like a slap in the faceWelcome backGuess whatNone of these niggas real as meI think i might line em upWhat you saySuck a dickScuse me if I"m bein rudeLet me check my attitudeScues me Mr. AttitudeWhy you got an attitudeCan"t stand the rapper niggersThinking that its trap a niggaThese never ever never everNever had a pack niggasI"m tellin youWhat they do to youWhat"s happening?Wut up dudeIve been doneYou too slowI"m too fastMovin like slim fastKeep my Ronald Williams cashIn a black Chevy bagWelcome backGuess whos back and he is shinin on you niggasWelcome backGuess whos back and he is stuntin on you hoesWelcome backYea I"m back and I"m shinin on you niggasWelcome backYea I"m back and I"m stuntin on these hoesBeen on my grizzy but now I"m back in the placeAnd if you show me the money ill put this brick in your faceAnd if ya aint got mo money ill put this strap in your faceAnd believing these niggas is like a slap in the faceWelcome backSo kind so kindThank you very muchActin like i lost touchI aint never lost touchDam sure aint lost muchMater of fact i still got itYea you know im still ballinCatch me in my black TBut im a business manCatch me at the kitchen table weighing out my business planYoung what your dentist planWhats wrong with your pussy niggasNeedin my opinions manOk here we go againStreet status necessaryStart talking blow againGot that bureau if the niggas sellin blow againStreets think its necessary that a nigga blow a manWelcome backGuess whos back and he is shinin on you niggasWelcome backGuess whos back and he is stuntin on you hoesWelcome backYea I"m back and I"m shinin on you niggasWelcome backYea I"m back and I"m stuntin on these hoesBeen on my grizzy but now I"m back in the placeAnd if you show me the money ill put this brick in your faceAnd if ya aint got mo money ill put this strap in your faceAnd believing these niggas is like a slap in the faceWelcome backIn my yayo in my kitchenSky storch every whereWhite fat black topKung Foo panda bearCall me sense I got the taiquondoMy Angelina shit I want that Brad Pitt doughI said so funny same time snow moneyAint nuthin funny bitchIm on my money shitA movie star bitchWe can make our own flickI just came to beat it downYou play with your own shitYea ok I"m bout to wild outCall it a v neckWhyyTook a nine out750 luck750 headBitch I dont even know what 750 isWelcome backGuess whos back and he is shinin on you niggasWelcome backGuess whos back and he is stuntin on you hoesWelcome backYea I"m back and I"m shinin on you niggasWelcome backYea I"m back and I"m stuntin on these hoesBeen on my grizzy but now I"m back in the placeAnd if you show me the money ill put this brick in your faceAnd if ya aint got mo money ill put this strap in your faceAnd believing these niggas is like a slap in the faceWelcome backYeahhhWe told you niggas rightAh ha hahttp://music.baidu.com/song/2253839

a welcome booklet ----- 一本受欢迎的手册an information booklet---- 说明手册note down-----记录下来

a boon to 恩赐 福利 利益welfare of 。。They did everything for the welfare of their children. 福利（社会） 幸福..

Welcome to our booth 网络 欢迎您到我们摊位。; [例句]Welcome every friend to our booth then!欢迎每一位朋友前来参观！

Welcometoourbooth网络欢迎您到我们摊位。;[例句]Welcomeeveryfriendtoourbooththen!欢迎每一位朋友前来参观！

建议你去论坛找大神帮你吧我一直用的是应用宝中一键root权限的方法想要使用肯定先要下载应用宝你可以选择下载应用宝再选定其中的一键root软件或者直接下载独立的一键root工具包， 下载完成之后用数据线连接和搜集和电脑并打开一键root工具接下来工具会自动识别您的手机并准备Root备份完成后再点击开始ROOT若已备份，请直接点击跳过开始Root

butt weld pipe elbow是什么弯头对焊弯头

I will come back soon翻译：我很快就回来

why do you like comedy ?为什么你喜欢喜剧？

地上凿洞：由于钻孔等的操作而在陆地上挖掘出来的一个洞。这个地上凿洞也许可以代表整个钻井孔（当没有侧钻出现的时候），或者是一个侧钻的扩展。这个地上凿洞是从起始点（即初始地上凿洞所在的地表位置，或者是侧钻的起始点）一直延伸到终点（即井底）。钻井孔：一个独立而具有方向的路径；这条路径是从钻过孔的地上凿洞的底部延伸到地表。而且这条路径不能有自我重叠或是交叉。井：由于某种目的，在地上进行钻井工作，而造成的钻井孔，所坐落的独立的地表位置。这个目的可以是（1）寻找或出产地下能源，（2）为出产地下能源提供相关的服务。侧钻：起源于另一个与初始凿洞方向相反的地上凿洞。可以参考百度百科的解释：侧钻是在油水井某个预定井段的套管一侧开窗，通过窗口钻出新的井眼，然后在这个新井眼中下尾管固定井的全部过程。侧钻工艺技术就是在油水井的某一特定深度固定一个斜向器，利用其斜面造斜和导斜作用，用铣锥在套管的侧面开窗，从窗口钻出新井眼，然后下尾管固井的一整套工艺技术。侧钻施工主要包括：侧钻前井眼准备、固定斜向器、套管开窗、裸眼钻进、下尾管固井等工序，现分述如下。http://baike.baidu.com/view/4480778.htm哇~查了半天的专业术语，纯人工翻译，不知道翻译的内容是不是清晰，是不是对你有帮助~希望采纳哦~

根本不是同样的东西。x0dx0ax0dx0aElevator 特指电梯，或者升降机，是一种机械设备x0dx0ax0dx0astairwell特指建筑中为建造水泥（也可以是混凝土，钢结构）楼梯留下的竖井，一般的楼梯空间就可以是stairwell.

根本不是同样的东西。Elevator 特指电梯，或者升降机，是一种机械设备stairwell特指建筑中为建造水泥（也可以是混凝土，钢结构）楼梯留下的竖井，一般的楼梯空间就可以是stairwell.

歌手：Five & Queen 专辑：We will rock you Buddy you"re a boy make a big noise Playin" in the street gonna be a big man some day You got mud on yo" face You big disgrace Kickin" your can all over the place Singin" "We will we will rock you We will we will rock you" Buddy you"re a young man hard man Shoutin" in the street gonna take on the world some day You got blood on yo" face You big disgrace Wavin" your banner all over the place "We will we will rock you" Singin" "We will we will rock you" Buddy you"re an old man poor man Pleadin" with your eyes gonna make you some peace some day You got mud on your face You big disgrace Somebody better put you back into your place "We will we will rock you" Singin" "We will we will rock you" Everybody "We will we will rock you" "We will we will rock you" Alright

《wewillrockyou》buddyyou"reaboy伙伴，你是个男孩makeabignoiseplayinginthestreet在街头大吵大闹gonnabeabigmansomeday以后你长大成人yougotmudonyourfaceyoubigdisgrace脸上粘着泥巴你太不光彩了~kickingyourcanallovertheplaceT着罐子到处乱跑（哈哈这个歌曲我才知道CAN原来有罐子的意思）wewillwewillrockyouwewillwewillrockyou（这个怎么翻译都可以根据自己的想法吧~翻译成我们将会摇滚起来会很好吧）buddyyou"reayoungmanhardman伙计你是个结实的年轻人shoutinginthestreetgonnatakeontheworldsomeday在街上叫嚷着有一天要征服世界yougotbloodonyourfaceyoubigdisgrace你脸上沾着血你太不光彩了wavingyourbannerallovertheplace摇着旗子四处跑wewillwewillrockyouwewillwewillrockyoubuddyyou"reanoldmanpoorman伙计你是个贫困的老人pleadingwithyoureyesgonnamakeyousomepeacesomeday用眼睛恳求有1天能得到安宁yougotmudonyourfaceyoubigdisgrace脸上沾着泥巴你太不光彩了somebodybetterputyoubackinyourplace最好有人把你放回你的地方wewillwewillrockyouwewillwewillrockyou

elks in jewell meadows公园草地上的麋鹿请采纳如果你认可我的回答，敬请及时采纳~如果你认可我的回答，请及时点击【采纳为满意回答】按钮~~手机提问的朋友在客户端右上角评价点【满意】即可。~你的采纳是我前进的动力~~O(∩_∩)O，记得好评和采纳，互相帮助

成都新明商贸 代理的博世的产品，在成都市红牌楼九峰汽配城二期二幢一号，哈哈就在我们店附近所以很熟悉，我们是八号。我们是成都德天车业（还有间店成都德盛时代汽配行）主要代理的是西门子VDO德国大陆集团产品（电器和ATE刹车片等）和TMD泰明顿刹车片，TRW刹车盘片，MANN曼胡默尔滤清器，科德宝空调滤新器，安帕仕（上汽集团上海国际汽车零部件采购中心产品品牌）电话028-82838376欢迎来店参观。新明商贸就是类似专卖店形式。

毛巾的复数形式

英语谚语: Well fed wed bred 中文意思: 衣食足，知荣辱。 随机推荐10条英文谚语： The best teachers of humanity are the lives of great men 启迪人性最好的导师是伟大人物的生活。 The better the day the better the deed 日子越美好，事业越顺当。 The bough that bears most hangs lowest 枝头结果越多，垂得越低。 The brave man hazards his life but not his conscience 英雄的人可以冒生命危险，但不冒良心危险。 The brightest of all things the sun has its spots 金无赤足，人无完人。 The burden on likes is cheerfully borne 爱挑的担子，就愉快轻松。 The busiest men find (or have) the most leisure (or time) 最忙的人时间最多。 The cask savours of the first fill 先入为主。 The cat and dog may kiss yet are none the better 猫犬可相吻，莫逆却难成。 The catin glores catch no mice 戴手套的猫捉不到老鼠。 英语谚语: Well fed wed bred 中文意思: 衣食足，知荣辱。

炎性肠疾病的意思

Connie gets on well with others.康妮和别人相处得很好。

超级碗（Super Bowl）是美国国家美式足球联盟（National Football League，NFL）的年度冠军赛，一般是在每年1月份的最后一个或是2月份的第一个星期天举行。超级碗是比赛双方所争夺奖杯的名字，亦是比赛的名字。美式足球的奖杯多数称为“碗”。例如洛杉矶的“玫瑰碗”是每年一月一日举行的大学美式足球比赛。举行的球场因此亦称为玫瑰碗球场。第一届超级碗，旧称AFL-NFL 世界冠军赛（AFL-NFL World Championship Game），于1月15日1967年举行，由NFL冠军与竞争对手美国美式足球联盟（American Football League，AFL）冠军来争夺“职业美式足球世界冠军”（World Championship of Professional Football）。在两个联盟于1970年合并后超级碗成为NFL的冠军赛。

Intestine指肠道，小肠大肠都是用这个表示，而bowel一般用于和排泄物相关bowelfunction特指排便功能，moveonesbowel也是类似的，有时也可通用个人观点，希望能帮的上忙

Change in bowel habit , or blood in urine or stool 大小便习惯改变或大小便带血 Change in urinary or bowel habits or blood stains in such excretions 大小便习惯改变或大小便带血； A change in stool or bowel habits can be created by the mass effect 包块影响的结果是大便习惯的改变。 Cultivate regular bowel habits and keep intestine clean and healthy 保持肠胃健康，养成每天上厕所的习惯。 Sudden change of bowel habit , e . g . alternative diarrhea and constipation 大便习惯明显转变，例如持续反覆地便秘或与肚泻 Sudden change of bowel habit , e . g . alternative diarrhea and constipation 大便习惯明显转变，例如持续反覆地便秘或与肚泻 5 bowel habits 5排便习惯 Seek the advice of a doctor . constipation is mon among the elderly but the above tips can help you to maintain a normal bowel habit 便秘在长者中较为普遍，但只要注意上述要点，养成良好生活习惯，也能令排便自如。 Seek the advice of a doctor . constipation is mon among the elderly but the above tips can help you to maintain a normal bowel habit 便秘在长者中较为普遍，但只要注意上述要点，养成良好生活习惯，也能令排便自如。 If the bowel habit changes suddenly or if you pass out blood or mucus , it may be the signal of some serious disease of the bowel , e . g . cancer 倘若大便习惯突然改变，甚或便中有血或黏液，均应尽早求诊，切勿讳疾忌医，因为这些可能是肠癌的徵兆。

在地下深处, 在地壳底下

他第一次漂洋过海,一个个古老的传说纷纷应验了,他吓得屁滚尿流. bowel turn to water是吓得屁滚尿流的意思,scare the shit out of me!

你有没有见6过一x辆车i是怎么c从6一u个v空旷的乡v村街道上c疾驶而过？ 2011-10-25 19:08:11

bowel是什么意思：肠;内部最深处1、Irritable bowel syndrome seems to affect more women than men 女性比男性更易患肠易激综合征。2、The boy was suffering from a bowel obstruction and he died. 这个男孩因患肠梗阻死去了。3、Research shows that a high-fibre diet may protect you from bowel cancer 研究表明高纤维饮食可预防肠癌。4、Try to make sure your bowel motions are regular and that you avoid any constipation. 尽量保证大便规律，避免便秘。5、Lyn went off into the dark bowels of the building. 林恩进入了这座漆黑的建筑物的内部。6、Keep one"s bowels open. 保持大便通畅。

歌曲名:Get-Well-Cards歌手:Conor Oberst专辑:Conor OberstConor Oberst - Get Well CardsA peacock, people left the plumes in a pileThey look good to foldAnd the Gulf water"s warm like a bathtubFull of lavender and epsom saltSee a bleach blond boy put his longboard downHelp his girl get her sunscreen onI thought about you in your little houseThink you"re lonely but I could be wrong and...I wanna be a bootleggerWanna mix you up something strangeBraid your hair like a sisterMaim you like a hurricaneRight there, that"s the postman sleeping in the sandHe"s got a letter to deliver, but I can"t stay madRight there, that"s the postman sleeping in the sandHe"s got a get-well-card to deliverHe"s gonna do it by hand, he"s gonna do it by handNow they drive the cars up and down the beachIt"s ridiculous and everybody knowsHear the Mustangs rev at the four way stopYou get ghosted when the light says goBut in a town like this, in the chequered flag dawnIt"s so empty you could make somebody dreamSo maybe it"s you, in your four-post bedFound asleep with still blood in your teeth and...I wanna be your happinessI wanna be your common sense painWrap your head in a picket fenceRebuild after the hurricaneRight there, that"s the postman sleeping in the sandHe"s got my letters to deliver, but I"m still not madRight there, that"s the postman sleeping in the sandHe"s got a get-well-card to deliverHe"s gonna do it by handHe"s gonna do it, he"s gonna do itHe"s gonna do it, he"s gonna do itHe"s gonna do it, he"s gonna do it... by handOhRight there, that"s the postman sleeping in the sandHe"s got a letter to deliver, but I can"t stay madRight there, that"s the postman asleep in the sandHe"s got a head stack now to deliverHe"s wants to do it by handHe"s gonna do it, he"s gonna do itHe"s gonna do it, he"s gonna do itHe"s gonna do it, he"s gonna do itHe"s gonna do it, he"s gonna do it... by handhttp://music.baidu.com/song/9382118

词组be good at...的用法

歌名：Oh Well歌手：BOYZ II MENSince you"ve been gone I"ve been lonelyLonging to be with you onlyMaybe there"s still is a way I can find you and sayJust how I feel.I can"t believe that"s it"s overWish somehow I could have showed herAll that was inside of my heart "stead of playing the gamesYou might have stayedFunny just the other night I was thinkingI wonder if you ever think about meI call you on the phone, there"s no answerOh Well, there"s still tomorrowOh Well, I"ll try againOh Well, maybe just maybe…Since you"ve been gone I"ve been lonelyLonging to be with you onlyIf there"s a way I could beg you to stay would you pleaseStay with me.I was thinking maybe I could come overHoping we could finally work this outEven if tonight we don"t find an answerOh Well, there"s still tomorrowOh Well, I"ll try againOh Well, maybe just maybeIf I had another chanceWe would stand hand in handYou"ll be my girl and I"ll be your manOh Well, maybe just maybe we can.I still call you on the phone still no answerMaybe later on I"ll try one more timeOr am I just a fool to keep tryingOh Well, there"s still tomorrowOh Well, a fool"s what I amOh Well, maybe just maybeOh Well, there"s still tomorrowOh Well, I"ll try againOh Well, maybe just maybeSee if I got down on my knees and gave you every little part of me.Oh Well, there"s still tomorrowOh Well, I"ll try againOh Well, maybe just maybeSince you"ve been gone I"ve been lonelyhttp://music.baidu.com/song/2150360

新诗1 既然你已经走了我一直在孤独 渴望与你同在只 也许还有一种方法我可以找到你说 只是我的感觉 我不能相信这是在 希望我能以某种方式表明她 所有这一切是在我的心 "代替玩游戏 您可能会留 乙条第1 有趣的，公正的其他夜 我想 不知道如果你想 关于我 我请你一个电话 没有答案 钩1 噢以及还有明天 噢以及我会再试一次 噢以及也许只是也许 新诗2 既然你已经走了我一直在孤独 渴望与你同在只 如果有一种方式我可以请您留 请你留在我 乙，第2节 我想也许我 可能会超过 希望我们终于可以 工作了这一点 即使今晚我们不 找到答案 钩1 大桥 如果我有一次机会 我们的立场 携手共进 您想成为我的女孩 我会是你的男人 噢以及也许只是也许我们可以 乙，第3款 我还是打电话给你的电话 仍然没有答案 也许以后我会尽力 一次是 还是我只是一个傻瓜 将继续尝试 钩2 噢以及还有明天 噢以及傻瓜就是我 噢以及也许只是也许 噢以及尽管如此，仍然有明天 噢以及我会再试一次 噢以及也许只是也许 如果我看到了我的膝盖上 并给您每一个小部分箱 噢以及还有明天 噢以及我会再试一次 噢以及也许只是也许 既然你已经走了我一直在孤独

歌曲名:Welcome To The Farm歌手:Luke Bryan专辑:Doin My ThingLuke Bryan - Welcome To The FarmWe"ve been up here in this city.Livin" in the grease and grime.Girl it"s a doggone pityWe haven"t been gettin" any time.I"ve been preachin" bout back home.It can make you feel so alive.I"ll tell ya what we can do,Baby let"s me and you take a little driveWelcome to the farmWhere you can roll your windows downBaby take my arm,ain"t hearin" nothin" such a beautiful sound?We can take the tractor down the road,go up around the bendLay by the river, crawl up in the barnWelcome to the farm, yea yeaThere ain"t no card in your pocket.If your gonna turn that old gas pump onIt"s cash for everything around here.That"s just the way it"s been for so longHow"d you like that coke in a green glass bottle?It"s colder than that plastic stuffWatch how miss Annie at the counterTells that story bout me wreckin" my truckThat straw in your hair,not an ounce of makeup on yaAs the sun goes down,girl I ain"t never seen you lookin so goodAs you do right nowDancin" on the step sideHow bout that blue skySo pretty it"ll make you cryhttp://music.baidu.com/song/3488081

音律或内涵方面。1、音律方面。doitwell这首歌的音律是十分好听的，很有设计感，很有范。2、内涵方面。doitwell这首歌是很有内涵的，歌词中有大家可以理解的内涵。

but ,is多余，可以这样说，We cannot do it well

歌曲名:Welcome歌手:The Who专辑:Tommy 2003 2Cd Deluxe Sacddisc 2 Of 2welcome to brandnew dayshine! bright! further alongwelcome to brandnew placewelcome to brandnew timefly! high! further alongwelcome to brandnew smile ah地図(ちず)に　のってないような　宝物(たからもの)　探(さが)しに出(で)かけよう希望(きぼう)あふれる　sunrise　目指(めざ)すは　遥(はる)かその先(さき)へ不思议(ふしぎ)な梦(ゆめ)を追(お)いかけて　その扉(とびら)の向(む)こう侧(かわ)　开(ひら)けばyes welcome!キミの目(め)に映(うつ)る　七色(なないろ)の虹(にじ)　辉(かが)くよbrandnew day!ぼくの手(て)にした　煌(きら)めく星(ほし)　瞬(またた)きだす涙(なみだ)　零(こぼ)れる时(とき)も　この気持(きも)ち　忘(わす)れないでいて瞳(ひとみ)揺(ゆ)らめく　sunset　愿(ねが)いは　遥(はる)かその先(さき)へどんな场所(ばしょ)にでも行(ゆ)けるし　何(なに)だってなれるはずだからyes welcome!胸(むね)の鼓动(こどう)　今(いま)　高鸣(たかな)る　とびっきりのhappybrandnew place!いつでもここへおいでよ　ずっと一绪(いっしょ)だからさぁ　光(ひかり)辉(かがや)けwelcome!キミの目(め)に映(うつ)る　七色(なないろ)の虹(にじ)　辉(かが)くよbrandnew time!ぼくの手(て)にした　煌(きら)めく星(ほし)　瞬(またた)きだすwelcome!胸(むね)の鼓动(こどう)　今(いま)　高鸣(たかな)る　とびっきりのhappybrandnew smile!いつでもここへおいでよ　ずっと一绪(いっしょ)だから「welcome!」作词：iyuna作曲：iyuna编曲：iyuna+伊吹ユキヒロ歌：茶太（终わり）http://music.baidu.com/song/1507798

brand-new 全新的，原来中间有个连字符的

brand new means totally new,in Chinese,全新的一年

重复用小室只有外周有一点细胞中间没有不知道是重复的原因还是胶或者细胞建议尝试一一排除或者追图

tig welding和argon are welding 是不一样的

懂key；淘哦；淘尔；我恩

donkey:dong keitowel:toutower:tao er warn:wer n

这个没用过，不好意思。不过给个网址，你看下，希望对你有帮助！

《The New Eldorado》（Murray）电子书网盘下载免费在线阅读链接：https://pan.baidu.com/s/1h-4Q_RaQqOmVuUK-h33OqA 提取码：6h80书名：The New Eldorado作者：Murray页数：386

材料与方法1. 1 Matrigel：Becton Dickinson Compary, - 20 ℃保存；Tanswell plate：Costar ,USA , # 3422 ,聚碳酯膜微孔直径为8μm；苏木素染液，梯度乙醇，二甲苯溶液。1. 2 趋化因子的制备 取传代第二天长势良好的NIH3T3细胞,无血清培养基轻柔漂洗两次; 加入无血清培养基,37℃,5 %CO2 培养24 h～48h，收集细胞培养上清；4 ℃,12 000rpm 离心,10 min ,取上清；0.22 μm 滤膜过滤除菌；分装,在- 20 ℃保存。1. 3 transwell 培养板准备 所有操作均需无菌操作。-20℃保存的Matrigel 在冰上保2℃～8℃过夜融化。在冰上用预冷的枪头吸取100μl Matrigel 加入冰预冷的300μl 无血清培养基中,充分混均。取上述稀释的Matrigel 25 μl 加入transwell 板上室,覆盖整个聚碳酯膜,37 ℃,30 min ,使Matrigel 聚合成胶。已铺好Matrigel的transwell 培养板置于37℃可保存2 周。1. 4 Matrigel 侵袭实验(Matrigel Invasion Assay)刺激后的各组细胞用PBS 漂洗3 次。以常规方法用无血清培养基制备单细胞悬液,5 ×105个细胞/ ml ,每组细胞各分为两部分。胎盘兰拒染实验,细胞活力需大于95 %。Transwell 培养板上室加入100μl 细胞悬液( 5 ×104 个) 并加入无血清培养基200 ul 。Transwell 培养板下室加入500μl 趋化因子,37℃,5 %CO2 培养24 h。用湿棉签轻轻擦去Matrigel 凝胶和聚碳酯膜上表面的细胞。小心取出上室,用线拴住,并做好标记,用冰预冷的甲醇固定30 min。苏木素染色1 min。梯度乙醇脱水(80 % ,95 % ,100 %) ,二甲苯透明。小心将聚碳酯膜自上室基底切取下来,置载玻片上中性树脂封片。附着于聚碳酯膜下表面的细胞在高倍镜下( ×400) 随机取6 个视野[1 ] 计数,取平均数。重复实验两次。

1. 1Matrigel：Becton Dickinson Compary, - 20 ℃保存；Tanswell plate：Costar ,USA , # 3422 ,聚碳酯膜微孔直径为8μm；苏木素染液，梯度乙醇，二甲苯溶液。1. 2 　趋化因子的制备　取传代第二天长势良好的NIH3T3细胞,无血清培养基轻柔漂洗两次; 加入无血清培养基,37℃,5 %CO2 培养24 h～48h，收集细胞培养上清；4 ℃,12 000rpm 离心,10 min ,取上清；0.22 μm 滤膜过滤除菌；分装,在- 20 ℃保存。1. 3transwell 培养板准备　所有操作均需无菌操作。-20℃保存的Matrigel 在冰上保2℃～8℃过夜融化。在冰上用预冷的枪头吸取100μl Matrigel 加入冰预冷的300μl 无血清培养基中,充分混均。取上述稀释的Matrigel 25 μl 加入transwell 板上室,覆盖整个聚碳酯膜,37 ℃,30 min ,使Matrigel 聚合成胶。已铺好Matrigel的transwell 培养板置于37℃可保存2 周。1. 4Matrigel 侵袭实验(Matrigel Invasion Assay)刺激后的各组细胞用PBS 漂洗3 次。以常规方法用无血清培养基制备单细胞悬液,5 ×105个细胞/ ml ,每组细胞各分为两部分。胎盘兰拒染实验,细胞活力需大于95 %。Transwell 培养板上室加入100μl 细胞悬液( 5 ×104 个) 并加入无血清培养基200 ul 。Transwell 培养板下室加入500μl 趋化因子,37℃,5 %CO2 培养24 h。用湿棉签轻轻擦去Matrigel 凝胶和聚碳酯膜上表面的细胞。小心取出上室,用线拴住,并做好标记,用冰预冷的甲醇固定30 min。苏木素染色1 min。梯度乙醇脱水(80 % ,95 % ,100 %) ,二甲苯透明。小心将聚碳酯膜自上室基底切取下来,置载玻片上中性树脂封片。附着于聚碳酯膜下表面的细胞在高倍镜下( ×400) 随机取6 个视野[1 ] 计数,取平均数。重复实验两次。

96孔细胞培养板培养瓶分类3595号 tissue culture plates 细胞培养板costar 培养瓶cat. catagory 类别

【【有oh的好听的英文歌】】你试一试下面的！！中国娃娃嗨歌--oh！oh！oh．britneyspears(布兰妮)-TillTheWorldEndsChange——金泫雅OneDirection--WhatMakesYouBeautifulOwlCity、CarlyRaeJepsen--GoodTime舞曲--RiseAndF...

well arranged [词典] 妥善；妥实；层次分明; [例句]Everything was well arranged.所有的事都铺排得停停当当。

多好的，就是说他们能够多好的编程信息。well是好 how well 就是说能够多好的怎么怎么样比如How well do these new ideas dovetail into the existing system?这些新意识与现存体制吻合得如何？

be good at…指某人擅长于（做）某事,at在这里是一个介词,可以在其后跟名词,be good at sth.指擅长于某事；或在其后跟动词-ing形式,表示做某事做得很好,很出色,其同义短语是do well in. 要注意at和in的固定搭配. She is good at English.她英语学得好. He does well in Chinese.他语文学得好.

你好,应该是：dobadlyin其实主要考的是well的反意词well是副词好地所以其反义词也是副词坏地badly~~~~~~~~~~～～~~~~祝你进步，如对你有帮助，请及时采纳~~~~~~~~~~~~~~~~~~

begoodat是擅长于什么dowellin是做什么事做得好.begoodat是接sth.dowellin是接doing不知道对不对哦.,.都有些忘记了///./

do well in = be good at 变否定在do前加don"t/doesn"t/didn"t等助动词的否定。be good at变否定在be动词后加not.例如：I do well in English.=I am good at English. I don"t do wellin English.=I am not good at English.

很简单good只能做形容词，意思是“好”well既可以做形容词也可以做副词well做形容词只有一种含义表示“身体健康”做副词意思是“好”

两者都有表示,擅长...的意思区别在于+后面的是动词还是名词例如:Idowellinplayingcomputergames.Idowelloncomput.一般情况下in+doingon+名词

你好！在……某方面干得好尽情享受最后，最终intruduceto如有疑问，请追问。

lilly is dowell in skying采纳喔0w0

一、从词性用法上比较。1.good只用作形容词，不用作副词，其副词形式用well。如：HespeaksgoodEnglish.他的英语说得好。HespeaksEnglishwell.他的英语说得好。2.well既可用作副词，也可用作形容词，用作形容词时，其用法很有限，只表示“健康的”，“恰当的”或“令人满意的”等。如：Sheisverywell.她身体很好。Thingsarewellwithus.我们事事如意。注：一般说来，well用作形容词时，只用作表语而不用作定语(如上例),但在美国英语中,well表示“身体健康”时有时也可用作定语。二、从相关短语用法上比较。1、begoodat与dowellin：两者均可表示“擅长”。如：Heisgoodatlanguages.他擅长学语言。Sheisgoodattellingstories.她擅于讲故事。Theyalldidverywellintheexamination.他们这次都考得很不错。注：表示擅长某一学科时，也可用begoodin.如：Heisgoodat[in]maths.他擅长数学。2.asgoodas与aswellas：用于本义时,两者均可表示“与……一样好”，但good是形容词，而well是副词。如：他说英语说得跟她一样好。正：HespeaksEnglishaswellasher.正：HisEnglishisasgoodashers.但是，用于引申义时，两者的意思很不相同：asgoodas意为“几乎”“差不多”(=almost)，aswellas意为“而且”，如：Theworkisasgoodasfinished.工作差不多干完了。HeasgoodassaidI"maliar.他无异于说我撒谎。She"scleveraswellasbeautiful.她不但漂亮,而且聪明。Aswellasbreakinghisleg,hehurthisarm.他不但摔断了腿，而且还伤了胳膊。认真编辑、认真排版的，希望能帮到你~望采纳，谢谢！

答：可以这样造句：He does very well in his math.

你可以根据以下各句了解它相应的用法：Whodoesbestinmathinyourclass?Doyoudowellinmath?Doeshedowellinmath?Didyoudowellinmathlastyear?

lilly is dowell in skying 采纳喔0w0

uff1fuff1fuff1fuff1fuff1fuff1f

小康的，富裕的

Do one thing at a time, and do well.意思是：一次做一件事，而且要做好。读法（谐音）：度晚僧艾t额塔姆，安d度未儿。

do well in playing pingpang

I do well in playing basketball.My sister does well in taking photos

1.springing up 2.have faith in 3.be associated with 4.wind...up 5.Are...familiar with

They do well at home.他/她们在家干得很好.

Sam does well in playing the piano.

themoeryoucanrealizethisthebetteryoucandowell是什么意思Answer ：你越能意识到这一点，你就能做得更好。

do well in 擅长......do是实义动词例如：I do well in English.She does well in Chinese.

原句是什么？and连词，可理解为“和”“也”“并且”do well做得好will表将来或者可能是名词表示“意愿”“意志”“毅力”等然而是do的原型那么可能是：并且将会做得很好

IN 是介词 介词后面跟 名词 动名词或者人称代词宾格 所以这里是DO WELL IN STH / DOING

D,第三人称单数,改为陈述句就是everybody does well at school

can do well 可以做的很好

did you do well 你做得好吗？

答：（在某方面）做/学得好。

不可以。dosthwell是对的，不过do不是助动词，而是实意动词，其中dosthwell是一个固定词组，do不能随意替换。dowelltodosth(espasawarning)actwiselyorprudentlyindoingsth（尤指提醒或警告）做某事时聪明或谨慎：Youwoulddowelltorememberwhoispayingthebill.你最好记住是谁付的帐。

有。it的位置使用错误。do是动词，后面要跟宾语，it要放在do的后面。把it修改到do后面才可以组成肯定句，doitwell。

dowellin是指在...做的好，doagoodjobin也是在...方面做得好，不同之处是dowellin后加动词，doagoodjobin后加名词。