10x pcr buffer8月10日拿出来做了一次pcr,之后一直放在-20,今天拿出来做的时候,解冻后管内液面上有白

GC buffer扩增出来的PCR产物可以用DHPLC进行突变检测吗
公司有说明做DHPLC的PCR产物中不应有DMSO等大分子物质；另外有网友说GC buffer中有DMSO。请问网友的说法对吗？如果可能请告知GC buffer 的配方组成。谢谢！

PCR为什么要在冰上操作?
做PCR的时候,如加各个反应液Taq酶,引物,dNTP,模板,PCR buffer等要在冰(冰盒)上操作?初次操作,敬请答复.

关于c++ 的两道概念理解题,
下列语句中,错误的是（）
a) const int buffer=256; b) const int temp;
c) const double *point; d）double*const pt =new double(5.5）；
关于函数重载,下列叙述中错误的是（）
a)重载函数的函数名必须相同
b）重载函数必须在参数个数或类型上有所不同
c)重载函数的返回值类型必须相同
d)重载函数的函数体可以有所不同

请教buffer和marker在跑电泳时的作用,还有loading buffer和loading marker在跑电泳时的作用,谢谢了
如题

2x sds-page loading buffer配方
非变性的2x sds-page loading buffer使用前加多少***?或者说1ml 2x sds-page loading buffer加多少***?

我要扩增一个基因GC含量较高,我想用GC buffer试试
现在用的是master mix 酶,buffer是否能与master mix 酶同用?

计算机英语中的 blits
还有这两句翻译下,
back buffer in video memory
allow hardware filled blits

求助一道汇编语言问题!

3、从无序数列中删除一个元素

实验内容

在BUFFER为首址的内存区中存放了一个无序字符串,其长度存放在第一个字节单元,在KEY单元内存放了要删除的一个字符,查找此无序字串,若找到则删除并作相应调整,若字串中无此字符则在FLAG单元内置FFH标志.

data segment

buffer db 0cH,'wcsegmentend'

key db 'm'

flag db ?

error db -1

data ends

stack segment para stack 'stack'

db 100 dup(0)

stack ends

code segment

assume cs:code,ds:data,ss:stack

main proc far

start: push ds

mov ax,0

push ax

mov ax,data

mov ds,ax

mov ax,stack

mov ss,ax

lea bx,key

mov al,[bx]

lea bx,buffer

mov cx,[bx]

jmp A4

A5: dec cx

jne A4

jmp A2

A4: inc bx

cmp [bx],al

jne A5

jmp A3

A2: mov dl,error

mov flag,dl

ret

A3: mov ah,[bx+1]

mov [bx],ah

inc bx

dec cx

jne A3

ret

main endp

code ends

end main

题目和编程如图所示,可我运行的时候却又跳出了dubug,这是怎么回事?

求pt->y==-2)g_over2=TRUE;recv(sock,buffer,655___35,0);
dLine,//comvoidsnake::initialize()score=0;

SacI XbaI双酶切体系,我以前用的50微升体系,现在想用20微升体系,关键是BUFFER的浓度,
我曾经用过50体系中两种酶各1，BUFFER用了5，那要是20的体系呢，BUFFER还是1X的么？

请问10*PCR buffer与10*taq buffer的区别
10*PCR buffer与10*taq buffer有什么不同呢?是不是都可以用于普通的PCR呢?

英语翻译
Typology
1) Buffer/safety stock
2) Cycle stock (Used in batch processes,it is the available inventory excluding buffer stock)
3) De-coupling (Buffer stock that is held by both the supplier and the user)
4) Anticipation stock
5) Pipeline stock (goods still in transit or in the process of distribution - have left the factory but not arrived at the customer yet)
Inventory examples
While accountants often discuss inventory in terms of goods for sale,organizations - manufacturers,service-providers and not-for-profits - also have inventories (fixtures,furniture,supplies,...) that they do not intend to sell.Manufacturers',distributors',and wholesalers' inventory tends to cluster in warehouses.Retailers' inventory may exist in a warehouse or in a shop or store accessible to customers.Inventories not intended for sale to customers or to clients may be held in any premises an organization uses.Stock ties up cash and if uncontrolled it will be impossible to know the actual level of stocks and therefore impossible to control them.
Whilst the reasons for holding stock are covered earlier,most manufacturing organizations usually divide their "goods for sale" inventory into:
Raw materials - materials and components scheduled for use in making a product.
Work in process,WIP - materials and components that have begun their transformation to finished goods.
Finished goods - goods ready for sale to customers.
Goods for resale - returned goods that are salable.
Spare parts
For example:
Manufacturing
A canned food manufacturer's materials inventory includes the ingredients to form the foods to be canned,empty cans and their lids (or coils of steel or aluminum for constructing those components),labels,and anything else (solder,glue,...) that will form part of a finished can.The firm's work in process includes those materials from the time of release to the work floor until they become complete and ready for sale to wholesale or retail customers.This may be vats of prepared food,filled cans not yet labelled or sub-assemblies of food components.It may also include finished cans that are not yet packaged into cartons or pallets.It's finished good inventory consists of all the filled and labelled cans of food in its warehouse that it has manufactured and wishes to sell to food distributors (wholesalers),to grocery stores (retailers),and even perhaps to consumers through arrangements like factory stores and outlet centers.

6*Glycerol DNA Loading Buffer与6*Sucrose DNA Loading Buffer的区别
还有6*Sucrose DNA Loading Buffer加了0.25% Xylene Cyanol FF与不加的区别,菜鸟请叫?

请问5TBE Buffer 溶液的配方?
谢谢

双酶切质粒后电泳
双酶切质粒pgl 6.24（5882bp）无共同buffer kPN I 2.5小时 bgl II 3.5小时 之后电泳 为什么出来1万多的带?跑的比原来质粒还有10000bp的marker还慢……怎么回事啊……求高人指点

lodging,seed filling period,germplasm,running buffer ,Ethidium bromide,CIM,significant,empirically determined LOD threshold

pcr试剂盒 保存温度
pcr试剂盒里面有：混合液一管（dntp、buffer等）、taq酶一管.
不小心把试剂盒放在4度里放了3天左右.
请问对实验会有影响吗?如果有的话,影响多大呢.

求这69句英文的意思
1.Size of scanbuffer[KB]:512[Can effect san speed a lot].
2.Fast scan on by default.
3.Don't scan memory that is protected with the No Cache option.
4.Keep low memory usage when doing an "Unkown lnitial Value scan"with Hyper Scan Scan the following memory types.
5.MEM_PRIVATE:Memory that is private.
6.MEM_IMAGE:Memory that is mapped into the view of an image section.
7.MEM_MAPPED:Memory that is mapped into the view of a section.[E.g:File mapping,slow].
8.Running the scan in a seperatr thread will give you a cancel button,and prevents.
9.ME from starvation.[meaning parts of the window turn white] but it also makes scanning a little slower.
10.Run scan in seperate thead
11.Thread priority:ldle
Lowest
Lower
Normal
Higher
Highest.
12.Select the file extensions you want associated with Cheat Engine.
13.CT [Standard Cheat Table].
14.CET [Moon1ight Engine T adle 2nd version].
15.CT2 [Moon1ight Engine T able 3nd version].
16.CT3 [Moon1ight Engine T able 3th version].
17.GH [Gamehack tables].
18.The following plugins are available.
19.Add new.
20.Delete.
21.There are 2 ways Moon1ight Engine can find the addresss of code that writes to a specific address.Enach type has it advantages and it's disadvantages.So choose which one suits you better.[or choose the one that doesnt qive you nroblems].
22.Use Debug Regiesters [aka Hardware Breakpoints].
Advantage:Not as memory intensive as the"Write Exceptions"type.And very high compatibility.
Disadvantage:May sometimes return awrong address.
23.Memory Access Exceptions.
Advantage:Finds every address that accesses the specified address.
Disadvantage:Memory intensive.
so slows down the game.
24.Try to prevent detection of the dabugger.
25.Handle beakpoints not caused by ME.
26.Show disassembler.
27.Show debugger options.
28.Use hardware breakpoints[Max 3].
29.Use int3 instructions for breakpoins [Unlimited].
30.Replace incomplete opcodes with nops.

我是新手,要做Xbal和XhoI双酶切实验,想请问一下buffer建议中2XTango是什么意思啊?
有没有哪位前辈能告知这两种酶的酶切反应体系,

DNA binding Buffer中的poly-dIdC是什么有什么作用?
分子生物学中,看到DNA-蛋白相互作用实验中用到了DNA binding Buffer,其中的poly-dIdC是什么有什么作用?

RIP-CHIP的polysome lysis buffer配方中的VRC是什么?
我要做RIP-CHIP,看到polysome lysis buffer配方中有一项是加400uM的VRC,这是什么?

核酸电泳时上扬缓冲液（loding buffer）的作用?
据说有三个

用fermentas的Kpn1和bamH1做双酶切,用的buffer是Buffer bamH1,做了几次都是质粒自连,怎么办

请问!博凌科为 的 Pfu DNA Polymerase（含预混Mg2＋的10×Pfu Buffer）怎么样啊?有没有谁能详细介绍下?

跪求高手指点一下,为什么我的PCR产物电泳图这个样子.引物是通用引物27F和1492r

体系25ul,buffer：2.5,dntp：2,引物：0.5,模板0.5,taq酶天根的（5U）0.2ul

预变性94℃,4min

变性94℃,40s

退火56℃,1min

延伸72℃,1.5min

三十个循环

之后72℃,10min

pcr buffer和其他成分混在一起（除了引物,模板和酶）,能长期放吗?我放在4度的,不知稳定吗?
最近有个pcr第一次拉出来了,还挺好,之后就再也拉不好了,体系、温度都是一样的,要么拉不出来,要么很弱.怀疑是不是mix buffer的原因.

c编程求出两条直线的交点
一.两条直线?
已知直线AB与直线CD
A、B、C、D的xy坐标存放在buffer[4] [2]中
怎么求出这两条直线的交点呢?
二.那如果4条直线呢?
已知直线AB与直线CD、直线EF与直线MN
A、B、C、D、E、F、M、N的xy坐标存放在buffer[8] [2]中

c++报错:Input string value is too large to fit into the buffer
[DBERR 错误：Input string value is too large to fit into the buffer :
insert into temp_info (c1,c2,c3,c4,c5,c6,c7) values(:c1 ,:c2,:c3,to_date(:c4,'YYYYMMDDHH24MISS' ),:c5,:c6,:c7) :Variable::c7,datatype in operator :CHAR]
数据库中针对c7是这样定义的：
create table temp_info (
c1 NUMBER(18) not null,
c2 NUMBER(5) not null,
c3 VARCHAR2(10) not null,
c4 DATE not null,
c5 VARCHAR2(20) not null,
c6 NUMBER(5) not null,
c7 VARCHAR2(2047)
)
程序插入时：
insert into temp_info (c1,c2,c3,c4,c5,c6,c7) values(:c1,:c2,:c3,to_date(:c4,'YYYYMMDDHH24MISS'
x05x05),:c5,:c6,:c7)

bool _fin = (buffer[0] & 0x80) == 0x80;这些都什么意思
_fin = (buffer[0] & 0x80) == 0x80;
_rsv1 = (buffer[0] & 0x40) == 0x40;
_rsv2 = (buffer[0] & 0x20) == 0x20;
_rsv3 = (buffer[0] & 0x10) == 0x10;
_opcode = (sbyte)(buffer[0] & 0x0f);
这些都解释下有木有

纯化中PBS buffer浓度
原来我纯化一个蛋白用的1*PBS~出来活性很差；后来听一个人说换成2*的,结果活性有了很大的提高,请问这两种BUFFER仅仅只是浓度差了些,为什么有这么大差别呢?