southern 和 northern 印迹杂交用的探针是DNA还是RNA?

T最后的ll2022-10-04 11:39:541条回答

southern 和 northern 印迹杂交用的探针是DNA还是RNA?
如题,能够分别说明最好.

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yan4798408 共回答了19个问题 | 采纳率94.7%
southern DNA
northern DNA or RNA
1年前

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Antarctica is in the most southern part of the world. It is
Antarctica is in the most southern part of the world. It is like nowhere else on the earth. It is much larger than Europe, and nearly twice the size of Australia. Antarctica is the coldest and windiest place in the world, even colder and windier than the North Pole. In summer, the sun shines for twenty-four hours a day, but in winter it’s completely dark for about three months. Very few plants grow there, but there is some wildlife, including whales, seals and penguins.
When Captain James Cook traveled around Antarctica in the 1770s by ship, he found no one living there. Today, a few scientists work in Antarctica, but they only spend a short time there.
Many scientists are studying the ozone layer , which is getting thinner and thinner worldwide. The biggest “hole” is above Antarctica, and weather there is getting warmer. Scientists think that this cold and lonely place can teach us a lot about the earth and how to keep the earth safe.
小题1:When did Captain James Cook travel around Antarctica?
A.In the 1670s. B.In the 1760s. C.In the 1770s. D.In the 1780s.
小题2:The underlined part “ozone layer” means “________” in Chinese.
A.冻土层 B.大气层 C.臭氧层 D.高压层
小题3:How many kinds of animals are mentioned in the passage?
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小题4:Which of the following is TRUE according to the passage?
A.Antarctica is much bigger than Australia in size.
B.Scientists spend a long time in Antarctica.
C.There is no plant growing in Antarctica.
D.In winter, it’s dark for about 70 days in Antarctica.
小题5:Why is the weather in Antarctica getting warmer and warmer?
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介绍一下这几种生物学鉴定方法NORTHERN 印迹杂交 SOUTHERN 印迹杂交 WESTERN 印迹杂交 离子交换层
介绍一下这几种生物学鉴定方法
NORTHERN 印迹杂交 SOUTHERN 印迹杂交 WESTERN 印迹杂交 离子交换层析 亲和鉴定 双脱氧末端终止测序法
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DNA印迹技术由Southern于1975年创建,称为Southern印迹技术,RNA印迹技术正好与DNA相对应,故被称为Northern印迹杂交,与此原理相似的蛋白质印迹技术则被称为Western blot. Southern印迹杂交是进行基因组DNA特定序列定位的通用方法.一般利用琼脂糖凝胶电泳分离经限制性内切酶消化的DNA片段,将胶上的DNA变性并在原位将单链DNA片段转移至尼龙膜或其他固相支持物上,经干烤或者紫外线照射固定,再与相对应结构的标记探针进行杂交,用放射自显影或酶反应显色,从而检测特定DNA分子的含量. Northern印迹杂交(Northern blot),是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.Northern 印迹杂交的RNA吸印与Southern印迹杂交的DNA吸印方法类似,只是在进样前用甲基氢氧化银、乙二醛或甲醛使RNA变性,而不用NaOH,因为它会水解RNA的2'-羟基基团.RNA变性后有利于在转印过程中与硝酸纤维素膜结合,它同样可在高盐中进行转印,但在烘烤前与膜结合得并不牢固,所以在转印后用低盐缓冲液洗脱,否则RNA会被洗脱. Western Blot中文一般称为蛋白质印迹.它是分子生物学、生物化学和免疫遗传学中常用的一种实验方法.其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色.通过分析着色的位置和着色深度获得特定蛋白质在所分析的细胞或组织中的表达情况的信息. 离子交换层析(Ion Exchange Chromatography简称为IEC)是以离子交换剂为固定相,依据流动相中的组分离子与交换剂上的平衡离子进行可逆交换时的结合力大小的差别而进行分离的一种层析方法. (不知道这个是不是你所提到的亲和鉴定?)将具有特殊结构的亲和分子制成固相吸附剂放置在层析柱中,当要被分离的蛋白混合液通过层析柱时,与吸附剂具有亲和能力的蛋白质就会被吸附而滞留在层析柱中.那些没有亲和力的蛋白质由于不被吸附,直接流出,从而与被分离的蛋白质分开,然后选用适当的洗脱液, 改变结合条件将被结合的蛋白质洗脱下来,这种分离纯化蛋白质的方法称为亲和层析. 双脱氧末端终止测序法:核酸模板在核酸聚合酶、引物、四种单脱氧碱基存在条件下复制或转录时,如果在四管反应系统中分别按比例引入四种双脱氧碱基,只要双脱氧碱基掺入链端,该链就停止延长,链端掺入单脱氧碱基的片段可继续延长.如此每管反应体系中便合成以共同引物为5’端,以双脱氧碱基为3’端的一系列长度不等的核酸片段.反应终止后,分四个泳道进行电泳.以分离长短不一的核酸片段(长度相邻者仅差一个碱基),根据片段3’端的双脱氧碱基,便可依次阅读合成片段的碱基排列顺序.
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小题4:We can replace (替换) the underlined word passion in the last paragraph with .
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B.exploring the Antarctica
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D.climate changes of the Antarctica
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south和southern意思和用法区别,请举例说明.
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south n.南部, 南 adj.南的, 南方的 adv.在南方, 向南方
down south 在南方, 到南方, 南下
go south with sth. [美俚]偷走某物, 私占某物; 带着某物潜逃
southern adj.南的, 南方的
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It’s roughly 1.6km north of the township at the southern entrance to Queen Elizabeth Park,a mere 200m from the beach.
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求四种分子杂交:southern\northern\eastern\western各是什么原理及作用?
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RT
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southern:用DNA作为探针杂交DNA.检测目标DNA的存在与否
northern:用DNA或RNA探针杂交RNA.检测目标RNA的存在与否
western:用抗体和目的蛋白结合进行杂交.检测目标蛋白的存在与否.
没有eastern杂交.少数人提议将IEF胶(即等电聚焦电泳)中的蛋白质转印到膜上的技术称为Eastern-blotting,但这一建议并未被广泛接受.
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Las Vegas is in the southern part of the state of Nevada,about 50 miles east of the California border and 30 miles west of the Arizona border.The city is divided into two main parts:a compact downtown called Glitter Gulch and the Strip,a corridor of hotels and casinos
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Jalim123 共回答了17个问题 | 采纳率100%
人手翻译!
拉斯维加斯位于内华达州南部,加利福尼亚州边境以东大约50英里,距离亚利桑那州西部的边界30英里.城市分为两个主要部分组成:一个人口紧密的市区称为格里特歌峡谷和加沙地带,一条酒店街和赌场.
综合填空The continent Antarctica is the most southern area of la
综合填空
The continent Antarctica is the most southern area of land on the Earth and is mostly covered with ice.People have never wanted to l 67 there because of its coldest temperature.But now scientists from all over the w 68 come to study the ice to learn more about the Earth’s history.Some scientists live on Antarctica for part of the y 69 .But they don’t usually stay there for more than six months b 70 there are six months of light and six months of darkness.
Antarctica usually has a good deal of f 71 weather without any wind at all.The strong winds are usually only in some parts of it.It is also i 72 to learn that it isn’t very wet there.There is very l 73 snow or rain.It snows on the ice in winter,but at other times Antarctica is d 74 ,just like the Sahara Desert.
In winter the sea around Antarctica freezes for t 75 of kilometers.But scientists tell us that the earth is getting w 76 .So in the future,there may be less ice in the Antarctic sea.When that happens,our weather will never be the same again.
woshiwo131年前1
晗雨氏 共回答了14个问题 | 采纳率92.9%
67、live,住
68、world,世界
69、year,年
70、because,因为
71、fine,晴朗的
72、interesting,有趣的
73、little,极少的
74、dry,干燥的
75、thousands,数千
76、warm,暖的
in what is now southern Iraq.这是从句吗,是什么从句?全句如下↓
in what is now southern Iraq.这是从句吗,是什么从句?全句如下↓
The first trenches that were cut into a 500-acre site at Tell Hamoukar.Syria,have yielded strong evidence for centrally administered complex societies in northern regions of the Middle East that were arising simultaneously with but independently of the more celebrated city-states of southern Mesopotamia,in what is now southern Iraq.
in what is now southern Iraq.求这句话是什么从句?
齐美拉1年前3
不是很会说 共回答了12个问题 | 采纳率100%
划分句子结构有助于理解你的问题:
1、主体句子结构:
【The first trenches 主语 】【that were cut into a 500-acre site at Tell Hamoukar.Syria,定语从句 】【have yielded 整个句子谓语】 【strong evidence for centrally administered complex societies 宾语,第二个定语从句的先行词】【in northern regions of the Middle East 介词短语,定语】【 that were arising simultaneously with but independently of the more celebrated city-states of southern Mesopotamia 定语从句】,【in what is now southern Iraq介词短语,地点状语】.
2、【in 介词】【 what is now southern Iraq 介词后面的宾语从句】
3、替换理
in 【the place 定语从句先行词】 【which is now southern Iraq 定语从句】
4、what 从句用法对比:
【What we need 主语从句】 is more practice.
Tell me 【what I can do for you 及物动词后面的宾语从句】.
The painting was once stored in【 what is a temple here 介词后面的宾语从句】.
Shanghai is not 【what it was ten years ago 表语从句】.
祝你开心如意!
有关Watson Won his most important game and became Southern Che
有关Watson Won his most important game and became Southern Chess Master in 1977…的阅读理解
zhuli88881年前1
bwyy4640 共回答了22个问题 | 采纳率95.5%
沃特森赢了他最重要的一局棋,成为了1977年的南部棋王
In some southern provinces ,it snowed very ______ last winte
In some southern provinces ,it snowed very ______ last winter A .heavy B .hard C.hardly D .strong
引领着我1年前1
宝贝的床单 共回答了27个问题 | 采纳率81.5%
B 只有这个副词适合
South 和southern相同吗?
South 和southern相同吗?
我滴一个英语老师说,south可以做形容词,但一定要把s大写,不然就是名词.即South=southern.但另一个英语老师说south不仅要大写,还要在前面加上the,the South China这样才可以.偶被搞混了,到底应该怎么用咧?
凤凰涅磐_ll1年前1
youdouzi 共回答了19个问题 | 采纳率89.5%
southern 只能做 形容词
south 可以做 名词 形容词 副词
用southern 基本可以用south 替代 反之不一定正确
This area is located in southern Manhattan.翻译
yangtze12181年前4
cool霉l 共回答了24个问题 | 采纳率100%
这个地区坐落于曼哈顿南部
希望对你有所帮助,有不会的再问吧
英语翻译It's tough to be openly gay in a conservative Southern t
英语翻译
It's tough to be openly gay in a conservative Southern town,he says.There have always been death threats,bullying and glares at his clothes and makeup.Oh,and he's always pushing someone's buttons.
J天亮2251年前1
缚鸟 共回答了24个问题 | 采纳率95.8%
push someone's buttons 是非正式用法,means:arouse or provoke a reaction in someone
Tuhao, dating back perhaps as far as the Southern Dynasty, _
Tuhao, dating back perhaps as far as the Southern Dynasty, _________ its new meaning last September.
A. put on B. took on C. carried on D. depended on
imqwtjcm1年前1
davyhzy 共回答了7个问题 | 采纳率85.7%
c
Hainan is in southern China.的同义句是什么
一迷惑1年前1
pashio 共回答了19个问题 | 采纳率89.5%
Hainan is in the south of China
the south of sw.和in the southern part of sw.有区别吗.
the south of sw.和in the southern part of sw.有区别吗.
不过我认为没区别,各位说的好像是to the south 与in the south
请问;
Guangdong is in the south of China.

Guangdong is in the souther part of China.
是大家说的这种区别吗。
I am not sure,FYI
知足方能快乐1年前5
oo合作mm组长 共回答了25个问题 | 采纳率92%
no
Most southern states had set up primary school systems by th
Most southern states had set up primary school systems by the late eighteenth century,but only in New England____and open to all students.A.primary schools were free B.free were primary schools C.were free primary schools D.were primary schools free
客ee长安1年前1
lucky_coco 共回答了19个问题 | 采纳率100%
选D.
句首有only,句子是部分倒装,故排除A、B.
又因为and后有open,故and前的词应与open并列.故选D.
the southern frontier of the desert was far to the north of
the southern frontier of the desert was far to the north of where it is now.请分析一下句子成分.
As recently as 6000 B.C.,the southern frontier of the desert was far to the north of where it is now.far to the north 怎么理解,to the north 是 far where it is now?怎么理解,the place which it is now?但翻译过来理解不了,“现在是的地方”?求解请问这句话整个翻译过来时什么意思
where it is now ,it is 如何翻译?
wangxiao851年前3
lwlml 共回答了13个问题 | 采纳率100%
far to the north of some place就是离什么地方的北边很远的意思呗.
这里把some place 换成了where it is now ,it 代之前面的“the southern frontier”,意思就是以前的南边在现在的南边以北很多.“where it is now ”在of后面做宾语成分所以就是宾语从句喽
Li ,a new student from a southern province.speaks a dialect
Li ,a new student from a southern province.speaks a dialect We ___hard to understand.
a .find b.find it was c.find its d.find him
唧唧喳喳唧唧歪歪1年前4
你是猪吧 共回答了17个问题 | 采纳率100%
B
Li是一个来自南方的学生,我们很难明白他讲的方言
121.A tsunami(海啸) _____ in some southern Asian countries in
121.A tsunami(海啸) _____ in some southern Asian countries in December,2004._____
homeless people had to stay in the open air.
A.has happened; Thousands of B.happened; Thousands of
C.has happened; About twelve thousand of D.happened; Twelve thousands
122.— Have you _____ had dumplings?
— No,not _____.
A.ever; yet B.ever; ever C.yet; yet D.yet; ever
123.— Look!
— Oh,hurry up,or we will miss it.
A.Here comes the bus.B.Here goes the bus.
C.Here the bus goes.D.The bus goes here.
124.of the students boys in our class.
A.One fifth; are B.One fifths; are C.First fifths; is D.One five; is
125.America is a country while China is a country.
A.developing; developed B.developed; developing
C.developing; developing D.developed; developed
126.You may my computer,but you mustn’t it to others.
A.lend; lend B.borrow; borrow C.lend; borrow D.borrow; lend
127.As parents,we should provide our children with food and clothes care for
their minds and behavior.
A.between; and B.either; or C.neither; nor D.not only; but also
128.Mr Chen has taught us English two years.I have been here two years ago.
A.since; for B.since; since C.for; since D.for; for
129.Thanks for giving me many presents.I haven’t never seen beautiful presents
in my life.
A.so; such B.such; so C.so; so D.such; such
130.Li Ming’s mother for 5 years.
A.has died B.died C.has been dead D.has dead
10题全讲解的追加分
lily0702161年前1
gdcclhj 共回答了22个问题 | 采纳率100%
121.B 海啸发生是个过去的事实,且有具体时间,所以用一般过去式.关于数字的表示方法,thousanda of+名词 是数千个什么,如果说具体的数,就只能用two(或其他)+thousand(一定是单数)+名词
122.A ever曾经 not yet还没有
123.A 这是here引导的倒装句,主语是名词the bus而不是代词,所以全部倒装,又因the bus是第三人称单数,所以用comes.试比较以下两个:here comes the bus.和here he comes.后者是因为主语是代词
124.A 表示分数的方法:基数词+序数词,当基数词大于1时,序数词用复数.基数词指one,two,three...序数词指first,second,third...比如:one third是三分之一,two thirds 是三分之二 后一个空的谓语用复数
125.B developed发达的 developing发展中的
126.D borrow是表示借走 lend是表示借给,借出
127.D not only...but aiso...不但...而且...
做家长的,应该不但要给孩子们提供衣食,而且还要注意他们的思想和行为举止 其它选项意思不符
128.C 陈老师已经教了我们两年英语,是现在完成式,two years是时间段
我自从两年前就在这里 since 自从 two years ago 是一个时间点
129.A 表示很多,这么多只能用so many,而没有such many的结构.
第二个用such+形容词+名词,我不是太确定的
130.A 这个应该是现在完成时吧,没有 has been dead和has dead 的用法.
in the south part of china的south需要变成southern吗
d0bs31年前1
58120719 共回答了17个问题 | 采纳率82.4%
不需要
This animal is to be found only in the Southern Hemisphere.
This animal is to be found only in the Southern Hemisphere.
为什么不能没有to be
jfylxc1年前2
枫花飘 共回答了16个问题 | 采纳率87.5%
这里to be表示“可以,可能”,相当于may,can.
例如:
The news is to be found in the evening paper.
这条消息可以在晚报上见到.
Such people are to be found everywhere.
这种人到处都有.
如果你把原话中的to be去掉,就变成is found,到底是现在时还是过去时呢?就如楼上所说,语法上是有问题的,具体我不细说了,长篇大论的.
如果不用这个用法,就是This animal can be found……
一般而言,这种to be的结构比can和may更能表达感情色彩!
英语缺词填空A storm dropped heavy rain on central and southern p__
英语缺词填空
a storm dropped heavy rain on central and southern p____ of china.
the radio says that a____ the xiangjiang river in hunan,floods caused by terrible r____ in the past weeks killed 25 people and 13 others were missing.
the chinese *** told some parts in yunnan and the city of chongqing a____possible heavy rain during the f____ few days.
in changsha water levels rose to 38 metres early monday morning,about 3 metres a____ the danger line.
in guangdong,at l____ 21 people died,while three were s____ hurt and seven were missing w____ heavy floods hit northern parts by saturday.
the cities of shaoguan ,meizhou and heyuan got the biggest l____ and many people became homeless.
about 620 million yuan was lost in the floods.guangdong has sent something u____ to meizhou,heyuan and shaoguan to help the people who lost homes b____their homes again.
沙-诺1年前1
felixz_2001 共回答了17个问题 | 采纳率94.1%
provinces across rain avoid forthcoming above least seriously when losing
urgent build
有关Watson Won his most important game and became Southern Che
有关Watson Won his most important game and became Southern Chess Master in 1977的阅读理解
米粉MM1年前1
wmcj6067 共回答了16个问题 | 采纳率87.5%
 Watson won his most important game and became Southern Chess Master (大师) in 1977.He was given the silver cup.
  “It isn’t rightly mine,” he said,when he was holding the cup.” “It was won two years ago when I was on holiday in…”
  “A family was staying at my hotel at that time.Mrs.Prig,the mother,was told that I played chess; and she begged me to give her young son a game.‘He’s only ten.’ she said,‘I’ve been told that he plays quite well.’
  “Well,as you can guess,I wasn’t too happy.A player likes the opponent (对手) to play as well as he does.But it was holiday time and I agreed to play.We placed the board (棋盘) in the garden.The game began.I hoped it would be quick----and so it was.
  “Isoon knew that David Prig was no learner.After ten minutes his sister came outside and began to play tennis against a wall.The boy seemed to lose interest in our game.He moved a piece (棋子) without care.I gave my attention to the board.
  “ Call me when you are ready,Mr.Watson,” he said.
  When I was ready?I looked up.He had gone off to play with his sister.I studied the board,and found I was driven into corner.So it went on with David:a quick move,then tennis,back to the board,then back to his sister.My difficult condition became impossible to change.I was beaten.Oh,so easily,by a ten-year-old chess player.He was the winner── in twenty-eight minutes.
  “David Prig,a name to remember.I had a chance to use his game today,and it won this cup for me.To him,of course,it is only one of a hundred,or perhaps a thousand,winning games.”
  1.When Mr.Watson said,“It isn‘t rightly mine.It was won …”,he meant _____two years before.
  A.he had played chess with a little boy for twenty-eight minutes
  B.he had had a chance to take part in an important game
  C.he had learned how to play chess from a child
  D.he had learned a good game from a child
  2.Before the game,Mr.Watson was quite sure that _________.
  A.the boy played as well as he did B.he would be Southern Chess Master two years later
  C.he could win the game easily D.the boy would win the game quickly
  3.Why did David play tennis while he was playing chess with Watson?
  Because he __________.
  A.had no interest in playing chess with adults (成年人) B.was not good at playing chess
  C.liked playing tennis much better than playing chess
  D.played chess much better than Watson
  4.From the story,we know that __________.
  A.David Prig was the real winner B.Mr.Watson was the real winner
  C.neither of them was the real winner D.both of them were the real winners
  5.Which of the following is NOT true?
  A.Sometimes young children can beat adults in playing chess.
  B.Watson thought he could win the game quickly,and so he did.
  C.Watson learned something from David and won the silver cup.
  D.Watson would never forget the name of the boy.
  Passage 49
  【答案与解析】这是一篇读后让人惊叹不已的故事.象棋大师Watson在旅馆时遇到了一个孩子.孩子的母亲请求Watson与儿子下一盘棋.Watson很不情愿地答应了.然而下棋过程中,那个孩子漫不经心地,还不时地跑出去和姐姐打网球.让Watson考虑之后再叫他.Watson后来处于僵局,最终也未能挽回.一个年仅十岁的孩子在二十八分钟里就胜了他.他非常佩服.后来,Watson用这个孩子的棋路获得了一个银杯.作者最后的“I had a chance to use his game today,and it won this cup for me.To him,of course,it is only one of a hundred,or perhaps a thousand,winning games.”这句话可以让人体会到Watson的心情,发人深思.
  1.D.本题需综合考虑下文所回忆的那局输掉的棋方找到答案.
  2.C.由作者在“I wasn’t too happy.A player likes the opponent to play as well as he does.”一句中所流露出的感情以及“But it was holiday time and I agreed to play.”等句子的暗示可判断作者根本没有把这个小孩子放在眼中.不难得出正确的答案.
  3.D.那小男孩即便是在“a quick move,then tennis,back to the board,then back to his sister.”这种情况下还是赢得了这局棋.这说明他的水平是明显高于对方的.
  4.A.本题难度稍大,但根据作者所表达的情感及“I had a chance to use his game today,and it won this cup for me.”一句可知A是最佳答案.
  5.B.由“I was beaten.… He was the winner--- in twenty-eight minutes.”等句子,可明显看出B是错误的.
如何把这句改成英文问句John C.Calhoun wanted southern interests respecte
如何把这句改成英文问句
John C.Calhoun wanted southern interests respected in new territories.
改成“谁想XXXX?”不是“John想怎么样?”
朵儿丝1年前1
淋雨的螃蟹 共回答了15个问题 | 采纳率100%
Who did want southern interests respected in new territories?
英语翻译Under Southern Skies演唱:妮基•韦伯斯特There is a peace in our he
英语翻译
Under Southern Skies
演唱:妮基•韦伯斯特
There is a peace in our hearts
And a hope in our hands
We're a family of children
we come from many different lands
Our time is just beginning,
Our race is yet to run,
But if you would take us with you
Then we have already won
Under the southern skies
Together in this land
Every voice in celebration
A family hand in hand under the southern skies
As one we rise
And turn our eyes to see
All the wonders of the future in a world of harmony
There's a great spirit rising
From the desert to the sea
As it sweeps across the southern land
It comes to you and me
Where the dreamers are the dreaming,
Where the race of the edge yet to run,
Every child can be a hero
If our world could live as one
大爱在江湖1年前1
蒙D87529 共回答了21个问题 | 采纳率85.7%
Under Southern Skies
同在南部天空下
Nikki Webster
There is a peace in our hearts
在我们心中有一个和平的梦想
And a hope in our hands
在我们手中有一个希望
We're a family of children
我们是一个儿童的大家庭
we come from many different lands
我们来自五湖四海
Our time is just beginning,
我们才刚刚开始
Our race is yet to run,
后面还有比赛等着我们
But if you would take us with you
如果你能和我们一起
Then we have already won
胜利就会在望
Under the southern skies
同在南方的天空下
Together in this land
在同一片土地上
Every voice in celebration
每一个声音都在庆祝
A family hand in hand under the southern skies
在这里手拉手聚齐的一个大家庭
As one we rise
当我们聚到一起
And turn our eyes to see
我们会看到
All the wonders of the future in a world of harmony
所有未来的希望和奇迹都会在这个融洽的氛围中实现
There's a great spirit rising
一种伟大的精神在聚集
From the desert to the sea
从沙漠到海洋
As it sweeps across the southern land
穿过南方的大地
It comes to you and me
来到你我之间
Where the dreamers are the dreaming,
这里梦想在升起
Where the race of the edge yet to run,
比赛的终点即将到达
Every child can be a hero
每一位少年都可以成为英雄
If our world could live as one
只要我们的世界有同一个心愿
原位杂交 与 southern 杂交的区别~
原位杂交 与 southern 杂交的区别~
如题
随便叫什么名字1年前1
zzrzzr 共回答了18个问题 | 采纳率72.2%
将标记的核酸探针与细胞或组织中的核酸进行杂交,称为原位杂交.
Southern印迹杂交是进行基因组DNA特定序列定位的通用方法.一般利用琼脂糖凝胶电泳分离经限制性内切酶消化的DNA片段,将胶上的DNA变性并在原位将单链DNA片段转移至尼龙膜或其他固相支持物上,经干烤或者紫外线照射固定,再与相对应结构的标记探针进行杂交,用放射自显影或酶反应显色,从而检测特定DNA分子的含量.
即前者是在组织中直接杂交,后者是提取出来再杂交
PCR和 Southern在RNA水平上的区别
sky32114211年前1
独倚栏杆 共回答了28个问题 | 采纳率89.3%
Southern是研究DNA的,Southern印迹杂交是进行基因组DNA特定序列定位的通用方法.一般利用琼脂糖凝胶电泳分离经限制性内切酶消化的DNA片段,将胶上的DNA变性并在原位将单链DNA片段转移至尼龙膜或其他固相支持物上,经干...

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