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同学你好，很高兴为您解答！　　knockout product，您说的这个英文词语在CMA的考试中比较常见，学会这个词语对考取英文CMA资格证书特别有帮助。这个词语的汉语意思是：淘汰的产品。　　希望高顿网校的回答能帮助您解决问题，更多CMA的相关问题欢迎提交给高顿企业知道。高顿祝您生活愉快！

在安装的时候要注意:一个是安装一个软件,另一个是安装在photoshop cs或 paint 下,我们的主要任务是安装在PHOTOSHOP（CS）Plug-Ins Filters 安装完毕后再分别执行KnockOut2_P1.exe和KnockOut2_P2.exe。前者安装到Knockout2.0的安装文件夹中，后者安装到PHOTOSHOP（CS）Plug-Ins Filters 注意：你直接运行EXE没用，你要打开你的PS，(见上图) 然后选择择滤镜-KNOCKOUT 2 ----载入工作图层，接下来，就出现了编辑界面了。(见下图) 安装完knockOut2.0之后,下面是尝试个人感受,好象有些地方做起来要强一些 第一:容易勾画,可以画的很仔细的. 第二:工具很强的,可以描绘各个区域的,有内部和外部的描绘工具,还有内部阴影的描绘工具. 第三:最后可以选择一张图做为背景 我也是刚接触这个,还不是很清楚里面到底有什么具体的东西,希望大家来讨论.希望password密码老师能够指导一下.我在这里只是想来个抛砖引玉而已。

转基因小鼠和knockin/out小鼠有什么区别转基因是过表达原有基因或者新表达原来没有的基因。knockin是改变原有基因的表达，基本上是做突变，但是不破坏原有基因的完整性，但是改变该基因的功能。而knockout是敲除该基因，即是原有基因失活，其中又有保留原基因部分完整性和不保留两种， 保留完整性通过剔除一部分序列但是保留该基因in frame读码框正确，这样的情况可能产生domain negative效应；不保留完整性则打乱原基因读码框，使得细胞产生non sence mediated decay而降解该基因mRNA，进而不产生该基因编码产物。这几种方法的原理以及操作不太一样，严格来说，目的也不完全一样。

安装过程是简单的，就是要在填写的时候要注意。在安装的时候要注意:一个是安装一个软件,另一个是安装在photoshopcs或paint下,我们的主要任务是安装在PHOTOSHOP（CS）Plug-InsFilters安装完毕后再分别执行KnockOut2_P1.exe和KnockOut2_P2.exe。前者安装到Knockout2.0的安装文件夹中，后者安装到PHOTOSHOP（CS）Plug-InsFilters注意：你直接运行EXE没用，你要打开你的PS，然后选择择滤镜-KNOCKOUT2----载入工作图层，接下来，就出现了编辑界面了。

基因knockout是一段基因被干扰了不能正常地被表达出来。（例如在一段基因里插入一段T-DNA就能影响基因的正常表达。）deletion应该就是基因里的一些核苷酸被删除掉了。

应该是软件出错了吧。建议重新下载一个安装看看，1、内含32和64两个安装包和一个注册机，请根据自己系统来下载相应安装包。2、下载安装包和注册机后，将其解压，先进行主程序的安装，双击Set-up.exe开始安装。注意:这里需要Creative Cloud帐号登录，如果不想注册小编建议拔掉网线或者禁用本地连接，拔掉网线出现以下界面，无需注册，等待安装..这个过程大概需要5分钟左右。3、安装完毕后，将注册机拷贝到Photoshop CC 2017安装目录，默认目录为C:Program FilesAdobeAdobe Photoshop CC 2017。4、运行注册机，选择的Photoshop 最新版本，大家无须担心，虽然不是2017版本，小编亲测可用，点击“install”,选择amtlib.dll，完成破解5、桌面上是不会出现快捷方式，大家可以点击“开始”右键“Photoshop CC 2017”选择发送到桌面快捷方式，下次就可以直接在桌面打开了Photoshop CC 2017：http://www.xue51.com/soft/138.html

滤镜一般都没问题。空虚滤镜我也用过。一般有以下几种情况：1、你用PS打开图片的图层是锁定的，可以按住ALT加左键双击图层上的小锁。2、你的图片尺寸过大，你可以将图片改小些试下。如果还不行可以加我的QQ 29430316

我是win8系统64位的，摸索了很久发现，绿色版knockout都不能安装或者安装后不能使用，从网上下载了一个安装版，竟然就可以了！建议你试试安装版的！我的photoshop是CS6的，本来装的是64位的，后来为了安装knockout，又装了一个32位的，目前将knockout装在ps32位的目录下，使用正常！64位尚未尝试！

KnockOut2是Corel公司出品的专业去背景软件，连极细的毛发都能从复杂的背景中分离出来。专业一点的术语是制作遮罩。基本简介所谓的去背，指的是将特定的主体从背景中分离出来， 以便进行其它的后续设计。例如，将人物从风景照之中抽离出来，以便更换背景。 目前，其他的影像处理软件，例如 PhotoImpact、PhotoShop ...等，其实也都可以进行去背的作业，但却不及 Knockout 来得功能强大及便利。在 Corel Knockout 之中，若背景较为单纯时，我们只要定义 Inside 及 Outside 两个选取区域，再按一下处理( Process )钮，通常便能完成去背的作业。 其去背的准确、快速及方便、容易程度，可谓其它软件都难以望其项背！

出现拒绝访问可能是你用某些工具设置了保护,无法更改之类。安装过程是简单的，就是要在填写的时候要注意。<一>在安装的时候要注意:一路"NEXT"，直到出现第一个安装路径，此处是安装Knockout 2.0软件的位置。下一步后会出现第二个安装路径，这个请指定Phtoshop文件夹下的Plug-Ins文件夹。安装完毕后再分别执行汉化KnockOut2_P1.exe和KnockOut2_P2.exe。前者安装到Knockout2.0的安装文件夹中（如上D:/Programe/Knockout 2.0），后者安装到Phtoshop文件夹下的Plug-Ins文件夹。成功后打开PS,工具栏滤镜下方会出现Knockout 2.0（注意：你直接运行Knockout 2.0没用，你要打开你的PS ：）。<二>1、解压到 PHOTOSHOP 的滤镜的Plug-Ins文件夹中；2、运行“绿化.bat”。

你好，很高兴为你解答。安装首先需要去下载Photoshop抠图滤镜KnockOut2.0压缩包，网上到处都有，下载一个靠谱的就行，这里因为不能附带网址，所以抱歉大家只好自己去下载此压缩包了；KnockOut2.0模式的是英文版，需要汉化才能转换为中文版。下面先来安装KnockOut 2.0；解压完KnockOut 2.0压缩包后，选择“KnockOut 2.0安装”文件；解压后可以看解压的内容，这是可以打开“SN”复制SN码，稍后就需要这个SN码。（这里贡献出SN码，sn: KO20CRD-0019351-RKU）点击里面的“Setup.exe”安装；同意协议并粘贴之前的SN码继续，第一个路径安装按照默认路径不要动，点“Next”继续下一步；到了第二个选择路径时注意，点击“Destination Folder”后面的“Browse…”进行路径选择，选择你安装Photoshop所在的系统盘的位置，通常在C盘，路径如下：C:ProgramFilesAdobePhotoshopCS增效工具（Plug-in）滤镜，将工具安装在滤镜里面，这一步非常关键，很多人之所以没有安装成功就是没有选对路径，所以一定要找准路径；然后Next继续，并在图中打勾选择并继续；PS抠图神器: KnockOut 2.0安装汉化和使用教程PS抠图神器: KnockOut 2.0安装汉化和使用教程4OK，出现这个弹出框就说明，你已经成功安装，点击“Cancel”就可以了，下面就验证此工具能否成功使用；那么现在就打开你的Photoshop，并打开一张照片，在滤镜下看看，就会发现多了一个KnockOut2的工具，此时可以看到这个是英文版的，为了方便操作，下面就来将此工具汉化，毕竟还是看中文比较舒服；PS抠图神器: KnockOut 2.0安装汉化和使用教程ENDKNOCKOUT2.0汉化汉化打开电脑上之前解压的Knockout2.0压缩文件，，可以看到文件“Knockout2.0汉化”文件，打开这个文件，首先双击打开“KnockOut 2_P1”，此时会有一个默认的路径出现，最好是将此路径放在你装KnockOut 2的C盘文件下，以确保工具可以准确找到对应的文件；可以看到，此时“KnockOut2_P1”成功安装，继续双击“KnockOut 2_P1”安装，同上面安装此工具一样，这一步也很重要，安装路径是在你的Photoshop滤镜下，路径一定要找准；看到弹出框，恭喜你，成功安装汉化插件。下面就去看一下是否汉化成功，进入Photoshop，可以看到，此时滤镜下的KnockOut 2已经是中文的，大功告成，现在可以找一些素材来试一下了。希望能帮到你，求采纳。

。。KNOCKOUT是一个抠图用专用滤镜。。你在网下一个。然后在PS里添加它就能出现在你的滤镜里面就可以使用了。。

GD） 钟情了，钟情了，完全钟情了（TOP） 完全钟情了 完全钟情钟情钟情了看到这两个人 我也会悄悄的钟情不对 是手吗 还是我们 让你钟情了（GD） this is double double, double double,combo double double,double comboThey say bubble bubble,bubble bubble 你嚼的吱吱作响 眼睛转着转着（TOP） 大街上走过的那些小姐们 就连小子也叫喊着我的名字（GD:TOP!)我的RAP是大韩民国的旗号食品 追随着我的年轻人们 斑驳陆离又很甜蜜打破有色眼镜 我的two job的冒险与我相似的想成为明星 虽然追随着 但我已是电影明星虽然比起女人我更喜欢玩具 小看我的话会受伤的（I didn"t mean ah）我的最初的日子 和谐的我 连便利店的咖啡的名字也叫 TOP我黑色的瞳孔让你动摇了 我的一对睫毛 以及浓浓的烟熏妆都是天生的优势完全钟情了 完全钟情钟情钟情了 看到这两个人 我也会悄悄的钟情不对 是手吗 还是我们 让你钟情了（GD） this is double double, double double,combo double double,double comboThey say bubble bubble,bubble bubble 你嚼的吱吱作响 眼睛转着转着（GD） 拨开云彩走过来的G-DRAGON！飞吧 super board beat 上的孙悟空MIC是我的指挥棒 舞台上的拿破仑 I"LL BE WAITING SEE MY SPACE 第二个权志龙BLING BLING BLING 大体上那样随便的搭配都会成为海报都知道我的名字吧 （bravo my life) 首尔to uh 东京 拍了又拍 他们都叫我洪吉童周一二三四五六日 每天被日程折磨着（oh oh)可到了夜里我却又很寂寞 期待我的绯闻吧（哥哥）觉得那个哥哥很好 真是没品位 认知度还不如咱家的家虎我的解释等同于催眠（诱惑你的手语） 我的嗓音相当sweet medusa（TOP） 完全钟情了 完全钟情钟情钟情了 看到这两个人 我也会悄悄的钟情 不对 是手吗 还是我们 让你钟情了（GD） this is double double, double double,combo double double,double comboThey say bubble bubble,bubble bubble 你嚼的吱吱作响 眼睛转着转着（TOP）从出生那天起就有的天生的嗓音 TOP和GD是杏仁和巧克力D D D Diplo与我们的配合 不现实的昨夜那个梦里的童话从出生那天起就有的天生的嗓音 TOP和GD是杏仁和巧克力D D D Diplo与我们的配合 不现实的昨夜那个梦里的童话（GD） 钟情了 钟情了 完全钟情钟情钟情了（TOP） 完全钟情了 完全钟情钟情钟情了 看到这两个人 我也会悄悄的钟情 不对 是手吗 还是我们 让你钟情了（GD） this is double double, double double,combo double double,double comboThey say bubble bubble,bubble bubble 你嚼的吱吱作响 眼睛转着转着

歌曲名:Knockout歌手:Lil Wayne专辑:RebirthLil" Wayne Ft. Nicki Minaj - Knockout(Lil Wayne)Uhh,Yeahh,Uh ohh,Yehh,Hey barbie, ah, ah, ah, are you into black men?He- hey hey barbie! I can be your black kenWow! Cause" once you go black, you never go backShe wore my hair black, with girls in the backThen she threw that ass back, back, backThen i fell to the mat, and after thatEverything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)Hey barbay, can i call you barbra? HahaHey barbay! Can i call you tomorrow, and todayCause" once you go black, you never go backAnd once you go wayne , everybody else is whackShe threw that ass back, back, back, then i fell to the matAnd after that everything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Nicki Minaj)Awe fuck itGive me that damn bucketWh-when i throw this pussyYou better not start duckinOh yoHead on with the blowLo-look out in the crowdAnd everybodys yellin noGrab him by the locksAnd give us a good boxThe head of the below the beltI wanna give him good tockTock, tockGive him good tockAnd if it below the beltI wanna give him good tock1,2,3They yellin 1,2,3I done hit him with that motherfuckin court pieceTell em, tell em the mouth outTo late to cop outGive him more head than toothpaste like i locked out(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)So just knock me outSo just knock me outYeaJust knock me outSo just knock me outJust knock me outJust knock me outJust knock me outJust knock me outhttp://music.baidu.com/song/593303

歌曲名:Knockout歌手:Triple 8专辑:Knock OutLil" Wayne Ft. Nicki Minaj - Knockout(Lil Wayne)Uhh,Yeahh,Uh ohh,Yehh,Hey barbie, ah, ah, ah, are you into black men?He- hey hey barbie! I can be your black kenWow! Cause" once you go black, you never go backShe wore my hair black, with girls in the backThen she threw that ass back, back, backThen i fell to the mat, and after thatEverything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)Hey barbay, can i call you barbra? HahaHey barbay! Can i call you tomorrow, and todayCause" once you go black, you never go backAnd once you go wayne , everybody else is whackShe threw that ass back, back, back, then i fell to the matAnd after that everything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Nicki Minaj)Awe fuck itGive me that damn bucketWh-when i throw this pussyYou better not start duckinOh yoHead on with the blowLo-look out in the crowdAnd everybodys yellin noGrab him by the locksAnd give us a good boxThe head of the below the beltI wanna give him good tockTock, tockGive him good tockAnd if it below the beltI wanna give him good tock1,2,3They yellin 1,2,3I done hit him with that motherfuckin court pieceTell em, tell em the mouth outTo late to cop outGive him more head than toothpaste like i locked out(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)So just knock me outSo just knock me outYeaJust knock me outSo just knock me outJust knock me outJust knock me outJust knock me outJust knock me outhttp://music.baidu.com/song/8516123

歌曲名:Knockout歌手:Kooll专辑:StarchildLil" Wayne Ft. Nicki Minaj - Knockout(Lil Wayne)Uhh,Yeahh,Uh ohh,Yehh,Hey barbie, ah, ah, ah, are you into black men?He- hey hey barbie! I can be your black kenWow! Cause" once you go black, you never go backShe wore my hair black, with girls in the backThen she threw that ass back, back, backThen i fell to the mat, and after thatEverything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)Hey barbay, can i call you barbra? HahaHey barbay! Can i call you tomorrow, and todayCause" once you go black, you never go backAnd once you go wayne , everybody else is whackShe threw that ass back, back, back, then i fell to the matAnd after that everything went back!(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Nicki Minaj)Awe fuck itGive me that damn bucketWh-when i throw this pussyYou better not start duckinOh yoHead on with the blowLo-look out in the crowdAnd everybodys yellin noGrab him by the locksAnd give us a good boxThe head of the below the beltI wanna give him good tockTock, tockGive him good tockAnd if it below the beltI wanna give him good tock1,2,3They yellin 1,2,3I done hit him with that motherfuckin court pieceTell em, tell em the mouth outTo late to cop outGive him more head than toothpaste like i locked out(Nicki Minaj - Chorus)Baby 1, 2, 3Tell em get the refereeCause he can, get the knockoutIf the telephone ringsIts emergancyCause he can, get the knockoutGet the knockout(Lil Wayne)So just knock me outSo just knock me outYeaJust knock me outSo just knock me outJust knock me outJust knock me outJust knock me outJust knock me outhttp://music.baidu.com/song/15286114

首先我们要将证件照片导入knockout软件，用选取工具选中需要修改的文字内容并且直接删除掉，然后打开填充工具将空白处填充好，最后用字体工具重新输入即可。Knockout是Corel公司出品的专业去背景软件，连极细的毛发都能从复杂的背景中分离出来。

这款软件叫“梦幻抠图” 用法： 1、解压到 PHOTOSHOP 的滤镜目录中（即是安装PS目录中找到plug-Ins文件夹里）； 2、运行“绿化.bat”。 再打开PS时可以滤镜中找到。

应该是色阶没有调整好。抠图图片成半透明，可能是因为曲线或者色阶没有调整好，把颜色调的对比都大一些，再进行通道抠图时候就可以了。Knockout是Corel公司出品的专业去背景软件，连极细的毛发都能从复杂的背景中分离出来。专业一点的术语是制作「遮罩」，所谓的去背，指的是将特定的主体从背景中分离出来，以便进行其它的后续设计。例如，将人物从风景照之中抽离出来，以便更换背景。软件，例如PhotoImpact、PhotoShop等，其实也都可以进行去背的作业，但却不及Knockout来得功能强大及便利。在CorelKnockout之中，若背景较为单纯时，我们只要定义Inside及Outside两个选取区。

英文单字一字多义，与不同介系词组合起来，会带有大相迳庭的语意。因此，英文老师 Adam 在 Engvid 上分享了「knock」的五种片语动词组合，以后看到「knock」就可以借由后面的介系词来判断句子的意思了！ 01. knock down （故意）使倒下 e.g He knocked down the guy who punched his brother. （他打倒那个揍他弟弟的家伙。） 02. knock over （故意或不小心）打翻 e.g He knocked over the wine on the table. （他不小心打翻桌上的红酒。） 03. knock up 使怀孕 e.g I knocked up my girlfriend, so now I have to marry her. （我让我女朋友怀孕，所以我现在得娶她了。） 04. knock out / knockout (n. / adj.) 打晕；打乱（规则；常态） / 出色的（女）人 e.g She knocked out the man who tried to harass her. （她打晕了那个企图要骚扰她的男人。） e.g She is a knockout! （她真的超级正！） 05. knock off / knockoff (n. / adj.) 打落（物品）；偷窃 / 仿冒品 e.g Be careful not to buy a LV knockoff here. （小心不要在这里买到 LV 包的假货。） knock down, knock down 中文, knock down 例句, knock down 意思, knock down 用法, knock down 翻译, knock down 英文例句, knock off, knock off 中文, knock off 例句, knock off 意思, knock off 用法, knock off 翻译, knock off 英文例句, knock out, knock out 中文, knock out 例句, knock out 意思, knock out 用法, knock out 翻译, knock out 英文例句, knock over, knock over 中文, knock over 例句, knock over 意思, knock over 用法, knock over 翻译, knock over 英文例句, knock up, knock up 中文, knock up 例句, knock up 意思, knock up 用法, knock up 翻译, knock up 英文例句, knock 片语, knock 相关片语, knock 英文片语, knockoff, knockout, phrasal verb

knockout汉语解释n.击昏，击倒； 绝代佳人； <俚>轰动一时的东西； 淘汰赛adj.击倒的，打倒的； 引人注目的； 使昏迷的； 迷人的knockout消除 来源《印刷设计辞典》所以印刷中的意思是将与背景相同的颜色印在重迭影像上以消除迭影的阴影。

击倒。knock有敲击的意思out是有淘汰的意思敲击淘汰也就是击倒的意思所以意思是击倒

不是。根据查询knockapp相关信息得知，knockapp不是骗局。Knock是杭州埃米网络科技有限公司开发的一款企业聊天工具，为企业员工提供高效、便捷、安全的通讯服务，可以随沟通，支持语音。视频、传输文件等。

这个学校里没有教室。.

Knock-knock-knock-knock, knockin?on the doorI抦 the one, I抦 the one you抣l be looking forKnock-knock-knock-knock, knockin?on the doorIt抯 the love, it抯 the love you抣l be waiting for!Knock-knock, I can keep on dreamingLet me in, I have to let you knowKnock-knock, cause my heart is beatingOh please, open up the doorOh baby, don抰 you run awayOh baby, I抣l keep厖Knock-knock-knock-knock, knockin?on the doorI抦 the one, I抦 the one you抣l be looking forKnock-knock-knock-knock, knockin?on the doorIt抯 the love, it抯 the love you抣l be waiting for!No more hiding my emotionsI have to let my feelings showSo sure, I抦 so mad about youOh please, open up the doorOh baby, don抰 you run away (away)Oh baby, I抣l keep厖Knock-knock-knock-knock, knockin?on the doorI抦 the one, I抦 the one you抣l be looking forKnock-knock-knock-knock, knockin?on the doorIt抯 the love, it抯 the love you抣l be waiting for!Knock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock, knockin?on the doorIt抯 the love, it抯 the love you抣l be waiting for!Oh baby, don抰 you run awayOh baby, oh babyOh baby, don抰 you run away (away)Oh baby, I抣l keep厖Knock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock-knockin?on the doorI抦 the one, I抦 the one you抣l be looking forKnock-knock-knock-knock-knockin?on the doorIt抯 the love, it抯 the love you抣l be waiting for! (x2)Knock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock, knockin?on the doorKnock-knock-knock-knock, knockin?on the door基因敲除基因敲，敲入？门 余抦一个，我一抦你抣升要寻找 基因敲除基因敲，敲入？门 它抯的爱情，它抯的爱你抣升在等待！ 基因敲，我可以继续做梦 请允许我，我要让你知道 基因敲，我的心原因是殴打 噢，请打开大门 噢孩子，你不要抰离家出走 噢孩子，我抣保持庞升 基因敲除基因敲，敲入？门 余抦一个，我一抦你抣升要寻找 基因敲除基因敲，敲入？门 它抯的爱情，它抯的爱你抣升在等待！ 没有更多的隐藏自己的情绪 我已经让我的感情显示 所以当然，我很生气抦你 噢，请打开大门 噢孩子，你不要抰离家出走（距离） 噢孩子，我抣保持庞升 基因敲除基因敲，敲入？门 余抦一个，我一抦你抣升要寻找 基因敲除基因敲，敲入？门 它抯的爱情，它抯的爱你抣升在等待！ 基因敲除基因敲，敲入？门 基因敲除基因敲，敲入？门 基因敲除基因敲，敲入？门 它抯的爱情，它抯的爱你抣升在等待！ 噢孩子，你不要抰离家出走 哦婴儿，婴儿哦 噢孩子，你不要抰离家出走（距离） 噢孩子，我抣保持庞升 基因敲除基因敲，敲入？门 基因敲除基因敲，敲入？门 基因敲除基因敲除敲入？门 余抦一个，我一抦你抣升要寻找 基因敲除基因敲除敲入？门 它抯的爱情，它抯的爱你抣升在等待！ （ χ2 ） 基因敲除基因敲，敲入？门 基因敲除基因敲，敲入？门 基因敲除基因敲，敲入？门

这句话字面意思是:你再多说一句话,我就揍扁你.(这里是形容对方生气的程度,实际意思是你要是再敢多说一句话,我就不让你好过,小心吃不了兜着走)One more :再..一个..word:单词,话.flat:扁平,平坦

歌曲名:Heart Of Innocence歌手:Jessica Simpson专辑:Sweet KissesHeart Of InnocenceJessica SimpsonOh, ooh yeahSometimes I wake upIn the dark nightAnd in my mindThere"s a picture of youI know someday this dreamWill come aliveSo for nowYour shadow will doI"ve never seen your faceBut I know you"re in my heartBaby someday someplaceI"ll hold you in my armsI have a gift for youSomething I have held ontoWaiting for your sweet caressNo ribbon has been untiedFrom all that I hold insideAnd only youWill possessThis heart of innocenceOh yeahI lay my head downOn a pillow of white (of white)Here in the darkI am cradled in peace (ooh)No loss from yesterdayTo haunt me at nightNo tears to dryNo sad memoriesIt isn"t hard to holdAnd I will safely keepThis strength that is in my soulUntil you come to meI have a gift for youSomething I have held ontoWaiting for your sweet caressNo ribbon has been untiedFrom all that I hold insideAnd only youWill possessThis heart of innocenceEvery night I wish before I fall asleep(Make a wish)That destiny will take your handAnd lead you to meOhI have a gift for youSomething I have held ontoWaiting for your sweet caressNo ribbon has been untiedFrom all that I hold insideAnd only youWill possessThis heart of innocenceOh, ohI love youhttp://music.baidu.com/song/8323777

这里应该选B题意是：摩托车把我从我的自行车上撞下来。knockoff是“敲落”的意思，强调的是“从某个地方跌落或掉落”例如：Hetriedhardtoknockoffthehoneycomb.他尽全力想把蜂窝给敲下来。而knockdown是“击倒,撞倒”的意思例如：Shewasknockeddownbyabus.她被公共汽车撞倒了.knockby&knockwith没有这种说法所以根据题意和重点词“mybike”，可以选出正确答案

“geneknockdown”是一种抑制基因的方法，翻译为“基因抑制”，而区别于“基因剔除－geneknockout"基因工程研究新利器“geneknockdown”RNA干扰(RNAinterference，简称RNAi)不但用途广泛，且为生物研究的有力工具。

转基因小鼠是指将某基因直接导入受精卵细胞，因此是全身各个组织都有表达，而knockin多指针对某一特定组织转入某基因。这和knockout与knockdown的区别是一样的，knockout是全身性缺失。

knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的英英释义是Verb:eliminate;"knockoutatarget"knockunconsciousorsenseless;"theboxingchampionknockedouthisopponentinafewseconds"destroyorbreakforcefully;"Thewindowswereknockedout"overwhelmwithadmiration;"Alltheguyswereknockedoutbyhercharm"empty(asoftobacco)byknockingout;"knockedoutapipe"。knockout的读音是英[n_ka_t]；美[nɑ_ka_t]。一、参考翻译点此查看knockout的详细内容击倒,使昏迷或入睡,彻底击败二、英英释义Verb:eliminate;"knockoutatarget"knockunconsciousorsenseless;"theboxingchampionknockedouthisopponentinafewseconds"destroyorbreakforcefully;"Thewindowswereknockedout"overwhelmwithadmiration;"Alltheguyswereknockedoutbyhercharm"empty(asoftobacco)byknockingout;"knockedoutapipe"三、例句Iheardhimknockouthispipeonthemantelpiece.我听到他在壁炉上敲出烟灰的声音。Pleaseseethepatternhehashammeredout.请参看他敲出的这个模子。Hewasdefeatedwithaknockout.他被击倒出局。Thebrickconnectedonherhead,knockingherout.砖击中了她的头部并把她击倒了。Hishopeswerenearlysnuffedout.他的希望几乎都被打破了。Hebrokethewindowsothathecouldshootout.他打破了窗户，便可以向外开枪了。Hesmiled,andhissmilerevealedthelossoftwofrontteeth,knockedoutbythebutt-endofagunatIsmail.上尉答道，他的鼻子涨红了，面露微笑，微笑时张开他在伊兹梅尔城下被枪托打落两颗门牙的缺口。TheotherboxerputJimoutinthethirdround.第三个回合拳击手将吉姆打昏了。Thepit-propfellonhisheadandknockedhimout.坑木掉在他头上，把他打昏过去。四、词汇搭配knockthebottomoutof破坏(驳倒knockthetaroutofsomeone痛揍某人,把某人狠揍...knockthespiritoutofsomeone挫伤某人的傲气...knocksomeone"seyeout给某人强烈印象...knockthestuffingoutofsomeone挫伤某人的傲气...knockdownanddragout彻底战胜knockhelloutofsomeone给某人无情的鞭打...knockout敲出knockone"spipeout敲出烟斗里的烟灰...knockthedaylightoutof吓得失去知觉...knockoutof从...中敲出...knocktheJesusoutofsomeone把某人打得半死...knockoutofthebox打掉投手knockout的相关近义词drug、exhaust、tire、snow、kayo、knockcoldknockout的相关临近词Knocke、knock、knockaboutwith、knockupagainst、knocktheendin、knockdownprice、knockintosb.、knock-outcore、knock-outgrid、knock-compound、knockdownprices、knockdownimport点此查看更多关于knockout的详细信息

knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的英英释义是Verb:eliminate;"knockoutatarget"knockunconsciousorsenseless;"theboxingchampionknockedouthisopponentinafewseconds"destroyorbreakforcefully;"Thewindowswereknockedout"overwhelmwithadmiration;"Alltheguyswereknockedoutbyhercharm"empty(asoftobacco)byknockingout;"knockedoutapipe"。knockout的读音是英[n_ka_t]；美[nɑ_ka_t]。一、参考翻译点此查看knockout的详细内容击倒,使昏迷或入睡,彻底击败二、词典解释1.使不省人事；使入睡Toknocksomeoneoutmeanstocausethemtobecomeunconsciousortogotosleep.e.g.Hewasarguingwithhisgirlfriendandshehithimwithafryingpan!Nearlyknockedhimout...他和女友争吵时女友竟拿煎锅砸他！几乎把他砸晕了过去。e.g.Thethreedrinksknockedhimout...3杯酒就让他昏昏入睡了。2.击败；淘汰Ifapersonorteamisknockedoutofacompetition,theyaredefeatedinagame,sothattheytakenomorepartinthecompetition.e.g.HenriLecontehasbeenknockedoutinthequarter-finalsoftheGenevaOpen...亨利·勒孔特在日内瓦公开赛四分之一决赛中被淘汰。e.g.TheIrishcamesoclosetoknockingEnglandoutoftheEuropeanChampionships.爱尔兰队险些将英格兰队从欧锦赛中淘汰出局。3.摧毁；使瘫痪Ifsomethingisknockedoutbyenemyactionorbadweather,itisdestroyedorstopsfunctioningbecauseofit.e.g.Ourbombershaveknockedoutthemobilelaunchers...我们的轰炸机摧毁了移动火箭发射台。e.g.Astormhasmaderoadstreacherousandknockedoutpower.一场暴风雨致使公路隐患重重且电力瘫痪。三、例句Iheardhimknockouthispipeonthemantelpiece.我听到他在壁炉上敲出烟灰的声音。Pleaseseethepatternhehashammeredout.请参看他敲出的这个模子。Hewasdefeatedwithaknockout.他被击倒出局。Thebrickconnectedonherhead,knockingherout.砖击中了她的头部并把她击倒了。Hishopeswerenearlysnuffedout.他的希望几乎都被打破了。Hebrokethewindowsothathecouldshootout.他打破了窗户，便可以向外开枪了。Hesmiled,andhissmilerevealedthelossoftwofrontteeth,knockedoutbythebutt-endofagunatIsmail.上尉答道，他的鼻子涨红了，面露微笑，微笑时张开他在伊兹梅尔城下被枪托打落两颗门牙的缺口。TheotherboxerputJimoutinthethirdround.第三个回合拳击手将吉姆打昏了。Thepit-propfellonhisheadandknockedhimout.坑木掉在他头上，把他打昏过去。四、词汇搭配knockthebottomoutof破坏(驳倒knockthetaroutofsomeone痛揍某人,把某人狠揍...knockthespiritoutofsomeone挫伤某人的傲气...knocksomeone"seyeout给某人强烈印象...knockthestuffingoutofsomeone挫伤某人的傲气...knockdownanddragout彻底战胜knockhelloutofsomeone给某人无情的鞭打...knockout敲出knockone"spipeout敲出烟斗里的烟灰...knockthedaylightoutof吓得失去知觉...knockoutof从...中敲出...knocktheJesusoutofsomeone把某人打得半死...knockoutofthebox打掉投手knockout的相关近义词drug、exhaust、tire、snow、kayo、knockcoldknockout的相关临近词Knocke、knock、knockaboutwith、knockupagainst、knocktheendin、knockdownprice、knockintosb.、knock-outcore、knock-outgrid、knock-compound、knockdownprices、knockdownimport点此查看更多关于knockout的详细信息

knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的英英释义是Verb:eliminate;"knockoutatarget"knockunconsciousorsenseless;"theboxingchampionknockedouthisopponentinafewseconds"destroyorbreakforcefully;"Thewindowswereknockedout"overwhelmwithadmiration;"Alltheguyswereknockedoutbyhercharm"empty(asoftobacco)byknockingout;"knockedoutapipe"。knockout的读音是英[n_ka_t]；美[nɑ_ka_t]。一、词典解释点此查看knockout的详细内容1.使不省人事；使入睡Toknocksomeoneoutmeanstocausethemtobecomeunconsciousortogotosleep.e.g.Hewasarguingwithhisgirlfriendandshehithimwithafryingpan!Nearlyknockedhimout...他和女友争吵时女友竟拿煎锅砸他！几乎把他砸晕了过去。e.g.Thethreedrinksknockedhimout...3杯酒就让他昏昏入睡了。2.击败；淘汰Ifapersonorteamisknockedoutofacompetition,theyaredefeatedinagame,sothattheytakenomorepartinthecompetition.e.g.HenriLecontehasbeenknockedoutinthequarter-finalsoftheGenevaOpen...亨利·勒孔特在日内瓦公开赛四分之一决赛中被淘汰。e.g.TheIrishcamesoclosetoknockingEnglandoutoftheEuropeanChampionships.爱尔兰队险些将英格兰队从欧锦赛中淘汰出局。3.摧毁；使瘫痪Ifsomethingisknockedoutbyenemyactionorbadweather,itisdestroyedorstopsfunctioningbecauseofit.e.g.Ourbombershaveknockedoutthemobilelaunchers...我们的轰炸机摧毁了移动火箭发射台。e.g.Astormhasmaderoadstreacherousandknockedoutpower.一场暴风雨致使公路隐患重重且电力瘫痪。二、网络解释1.击倒：90年代中期,FOSTER等以基因击倒(knockout)的整形技术,获得缺失blrl(blrl-1-)的小鼠,并发现食道癌这种小鼠缺少髂淋巴结,不形成或只形成部分形态不正常的派伊尔氏小结,同时,淋巴两性细胞游走到脾脏滤泡的过程受到严重损坏,2.敲空;(拳击中)击倒：knockoff把...敲掉;击倒;停工|knockout敲空;(拳击中)击倒|knowabout了解,知道...的情况3.knockout：ko;击倒三、例句Iheardhimknockouthispipeonthemantelpiece.我听到他在壁炉上敲出烟灰的声音。Pleaseseethepatternhehashammeredout.请参看他敲出的这个模子。Hewasdefeatedwithaknockout.他被击倒出局。Thebrickconnectedonherhead,knockingherout.砖击中了她的头部并把她击倒了。Hishopeswerenearlysnuffedout.他的希望几乎都被打破了。Hebrokethewindowsothathecouldshootout.他打破了窗户，便可以向外开枪了。Hesmiled,andhissmilerevealedthelossoftwofrontteeth,knockedoutbythebutt-endofagunatIsmail.上尉答道，他的鼻子涨红了，面露微笑，微笑时张开他在伊兹梅尔城下被枪托打落两颗门牙的缺口。TheotherboxerputJimoutinthethirdround.第三个回合拳击手将吉姆打昏了。Thepit-propfellonhisheadandknockedhimout.坑木掉在他头上，把他打昏过去。四、词汇搭配knockthebottomoutof破坏(驳倒knockthetaroutofsomeone痛揍某人,把某人狠揍...knockthespiritoutofsomeone挫伤某人的傲气...knocksomeone"seyeout给某人强烈印象...knockthestuffingoutofsomeone挫伤某人的傲气...knockdownanddragout彻底战胜knockhelloutofsomeone给某人无情的鞭打...knockout敲出knockone"spipeout敲出烟斗里的烟灰...knockthedaylightoutof吓得失去知觉...knockoutof从...中敲出...knocktheJesusoutofsomeone把某人打得半死...knockoutofthebox打掉投手knockout的相关近义词drug、exhaust、tire、snow、kayo、knockcoldknockout的相关临近词Knocke、knock、knockaboutwith、knockupagainst、knocktheendin、knockdownprice、knockintosb.、knock-outcore、knock-outgrid、knock-compound、knockdownprices、knockdownimport点此查看更多关于knockout的详细信息

Telescoping box-天地盖硬盒Ballotin box-抽屉式硬盒Knockdown box -可折叠木箱

knockdown的意思是：击倒；拆卸。knockdown的意思是：击倒；拆卸。knockdown的英英释义是Verb:causetocomeorgodown;"Thepolicemandownedtheheavilyarmedsuspect""Themuggerknockeddowntheoldladyaftersherefusedtohandoverherwallet"knockdownwithforce;"Hedeckedhisopponent"shatterasifbyexplosion。knockdown的读音是英[n_kda_n]；美[nɑ_kda_n]。一、参考翻译点此查看knockdown的详细内容撞倒,拆除,挣(钱)二、词典解释1.（被车）撞倒Ifsomeoneisknockeddownorisknockedoverbyavehicleoritsdriver,theyarehitbyacarandfalltotheground,andareofteninjuredorkilled.e.g.Hediedinhospitalafterbeingknockeddownbyacar...他被车撞倒后死在了医院。e.g.Adrunkdriverknockeddownandkilledtwogirls...一个酒后驾驶的司机撞死了两个女孩儿。2.推倒，拆掉，拆毁（建筑物）Toknockdownabuildingorpartofabuildingmeanstodemolishit.e.g.Whydoesn"thejustknockthewalldown?...他为何不干脆把墙推倒？e.g.Theyhavesinceknockeddowntheshack.从那以后他们拆掉了简陋的棚屋。3.降（价）；杀（价）；减（量）Toknockdownapriceoramountmeanstodecreaseit.e.g.Themarketmightabandonthestock,andknockdownitsprice...市场可能会抛售该股票，从而令其股价下跌。e.g.Itmanagestoknockrentsdownto$1persquarefoot.成功地将租金降到每平方英尺1美元。inAM,usuallyuse美国英语通常用bringdown三、例句Achildwasstruckdownbylightninginthestorm.暴风雨中，一个小孩被雷电击倒。Hemanagestoknockdownaguardandgrabaknife.他设法击倒了一个警卫，抓起了一把刀。Alecwasknockeddownbyabusyesterday.亚历克昨天被公共汽车撞倒了。Thecarelessmotoristrandownaboyonabicycle.那个粗心大意的汽车司机撞倒了一个骑自行车的孩子。Theyhavetorndownthatdangerouswall.他们已拆除了那堵危墙。Thecinemasheusedtovisithadbeenpulleddown.她常去的那家电影院已经拆除了。四、词汇搭配knockdownanddragout彻底战胜knockdown击倒knockdownprice成交价格,最低限价...knock-downargument使人无法反驳的论据...knocksomeonedownwithafeather使某人十分惊奇...knockdown的相关近义词dump、floor、coldcock、pushdown、deck、cutdown、pulldown、down、knockinto、blastknockdown的相关临近词knockup、knock、knockthedaylightoutof、knockforknockagreement、Knockoffeveryobjection、Knockupalivestockshed、knocksomeoneoutoftime、knockintomyoldteacher点此查看更多关于knockdown的详细信息

knockout 是基因敲除，knockdown 是基因沉默 是分子生物学中常用的两个方法

补充回答knockup:vt.敲门唤醒(筋疲力尽)例句：Wouldyoupleaseknockmeupat7o"clock?请7点钟敲门把我叫醒行吗?

knockdown是相对于knockout而言的一种技术，即通过microRNA抑制基因的表达，但并不能完全消除，故认为应当翻译为"抑制"或敲低。

区别是意思是不同。knockout英[nu0254kaut]美[nɑkau028at][词典]淘汰;击败;出局;使不省人事;[例句]Hewasarguingwithhisgirlfriendandshehithimwithafryingpan!Nearlyknockedhimout.他和女友争吵时女友竟拿煎锅砸他！几乎把他砸晕了过去。knockdown英[nu0254kdaun]美[nɑkdau028an][词典]拆卸;<非正>击倒;拒绝;<非正>降价;[例句]Hediedinhospitalafterbeingknockeddownbyacar.他被车撞倒后死在了医院。

knockdown〈非正〉击倒,撞倒Heknockedhisopponentdownwithoneblowinthecompetition.比赛中他一拳就打倒了他的对手。拆卸Theportabledeviceeasilyknocksdown.这种轻便装置容易拆卸。〈非正〉降价;减少Themanwasasking￡5forthedress,butIknockeditdownto￡4.50.那件衣服卖主索价5英镑,但我砍价后以4.5英镑买下了。卖时以击锤表示卖出TheauctioneerknockedthebureaudowntoafurnituredealerfromYork.拍卖者将写字台卖给从约克来的家具商。〈俚〉挣得,获得过Acleverlawyercanknockdown40,000dollarsinagoodyear.如果碰上好运气,一个精明的律师一年就能赚四万美元。〈俚〉偷;抢Hewantedtoknockdownabank.他想抢劫银行。使降低;使丢脸Shedoesnotwanttoknockdownhisself-esteem.她不想挫他的自尊。拒绝Theyknockeddownthesuggestion.他们把这条建议否决了。挥霍Sheknockeddownfortydollarsatarestaurant.她在一家饭馆花掉40美元。在燃烧区域边缘控制〔减弱〕火焰〔高温〕Therewasalowprotectingwalltoknockdowntheheatblastingfromthepitofthefurnace.有一堵低矮的护壁挡住从炉膛里喷出的火焰。

knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的意思是：敲空；击倒；打破；使筋疲力竭。knockout的英英释义是Verb:eliminate;"knockoutatarget"knockunconsciousorsenseless;"theboxingchampionknockedouthisopponentinafewseconds"destroyorbreakforcefully;"Thewindowswereknockedout"overwhelmwithadmiration;"Alltheguyswereknockedoutbyhercharm"empty(asoftobacco)byknockingout;"knockedoutapipe"。knockout的读音是英[n_ka_t]；美[nɑ_ka_t]。一、参考翻译点此查看knockout的详细内容击倒,使昏迷或入睡,彻底击败二、词典解释1.使不省人事；使入睡Toknocksomeoneoutmeanstocausethemtobecomeunconsciousortogotosleep.e.g.Hewasarguingwithhisgirlfriendandshehithimwithafryingpan!Nearlyknockedhimout...他和女友争吵时女友竟拿煎锅砸他！几乎把他砸晕了过去。e.g.Thethreedrinksknockedhimout...3杯酒就让他昏昏入睡了。2.击败；淘汰Ifapersonorteamisknockedoutofacompetition,theyaredefeatedinagame,sothattheytakenomorepartinthecompetition.e.g.HenriLecontehasbeenknockedoutinthequarter-finalsoftheGenevaOpen...亨利·勒孔特在日内瓦公开赛四分之一决赛中被淘汰。e.g.TheIrishcamesoclosetoknockingEnglandoutoftheEuropeanChampionships.爱尔兰队险些将英格兰队从欧锦赛中淘汰出局。3.摧毁；使瘫痪Ifsomethingisknockedoutbyenemyactionorbadweather,itisdestroyedorstopsfunctioningbecauseofit.e.g.Ourbombershaveknockedoutthemobilelaunchers...我们的轰炸机摧毁了移动火箭发射台。e.g.Astormhasmaderoadstreacherousandknockedoutpower.一场暴风雨致使公路隐患重重且电力瘫痪。三、例句Iheardhimknockouthispipeonthemantelpiece.我听到他在壁炉上敲出烟灰的声音。Pleaseseethepatternhehashammeredout.请参看他敲出的这个模子。Hewasdefeatedwithaknockout.他被击倒出局。Thebrickconnectedonherhead,knockingherout.砖击中了她的头部并把她击倒了。Hishopeswerenearlysnuffedout.他的希望几乎都被打破了。Hebrokethewindowsothathecouldshootout.他打破了窗户，便可以向外开枪了。Hesmiled,andhissmilerevealedthelossoftwofrontteeth,knockedoutbythebutt-endofagunatIsmail.上尉答道，他的鼻子涨红了，面露微笑，微笑时张开他在伊兹梅尔城下被枪托打落两颗门牙的缺口。TheotherboxerputJimoutinthethirdround.第三个回合拳击手将吉姆打昏了。Thepit-propfellonhisheadandknockedhimout.坑木掉在他头上，把他打昏过去。四、词汇搭配knockthebottomoutof破坏(驳倒knockthetaroutofsomeone痛揍某人,把某人狠揍...knockthespiritoutofsomeone挫伤某人的傲气...knocksomeone"seyeout给某人强烈印象...knockthestuffingoutofsomeone挫伤某人的傲气...knockdownanddragout彻底战胜knockhelloutofsomeone给某人无情的鞭打...knockout敲出knockone"spipeout敲出烟斗里的烟灰...knockthedaylightoutof吓得失去知觉...knockoutof从...中敲出...knocktheJesusoutofsomeone把某人打得半死...knockoutofthebox打掉投手knockout的相关近义词drug、exhaust、tire、snow、kayo、knockcoldknockout的相关临近词Knocke、knock、knockaboutwith、knockupagainst、knocktheendin、knockdownprice、knockintosb.、knock-outcore、knock-outgrid、knock-compound、knockdownprices、knockdownimport点此查看更多关于knockout的详细信息

击倒数8：比赛中，拳击甲方将对手乙方身体有效击倒在地，或对手除了脚以外的身体任何其它部位，如：手部，肘部等等部位有触地现象，均视为被击倒；此时台上裁判员立刻喊停，拳击甲方站立到拳台的中立角等待，裁判员则来到被击倒的乙方面前，用英语数数字1到8，结束数数字之前，如乙方主动由地面站立起来，并举起双手，既是表示自己没有问题还可以继续比赛；否则既说明乙方已无力再战，以失败告终。

1.将外源基因插入慢病毒载体2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞3.收集病毒液4.用病毒液感染靶细胞5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法6.获得稳转株

观察细胞中蛋白的变化，要转染后多久能看出来能抑制多长时间你需要自己检测。不同细胞、不同siRNA、不同转染试剂都会影响效率。但是假设你转染之后48小时或者72小时之后passage 细胞做MTT或者克隆形成实验，个人觉得是可以的（前提是你有48小时或72小时knockdown证据）。但是如果你knockdown效率不高的话，实验敏感性就大大扣（假阴性）。

去哪里找最出色的生命科学学术交流论坛？>>> 其实很早就看到你这个问题了，但是一直没有时间给你彻底的回答下，因为我们实验室就是做这个东西的，所以自认为还对它有点了解。今天有点时间给详细写出来吧。你所说的morpholino knock down的表中称呼应该叫做 PMO， 全称是Phosphorodiamidate Morpholino Oligomer。 是在九十年代初美国的AVI pharmacy开发出来的一种反义（Anti-sense）技术,其基本原理是把核苷酸上面的五碳糖环用一个吗啉环（morpholino）取代，同时对原有的磷酸基团也做了改变。使得这样一个DNA分子类似物可以以碱基配对配对的方式同RNA和DNA单链结合，但是由于结构的改变，使得整个分子不带有任何电荷，因此无法被任何酶所识别，包括DNase和RNase. 使得这类物质在细胞内有着极强的稳定性。在此基础上，如果你针对某一个基因的mRNA，设计一段反义的PMO寡聚物，在细胞内这个寡聚物和RNAi一样，通过与mRNA结合，来阻断这个mRNA的翻译，使得某个基因的在表达水平上被阻断，继而达到敲出基因的目的。但是这种技术与RNAi不同的地方是RNAi可以通过RNase H来实现目标mRNA的降解。而单独的PMO与mRNA结合并不能导致mRNA的降解。这就是PMO技术的基本原理。相比较起RNAi等其他反义技术，PMO有很多优点，比如，PMO不会被任何酶所降解，在细胞内稳定性极强，而且对细胞无毒副作用，并且不会和人工合成的RNA一样，会激活细胞干扰素的分泌，激起免疫应答。 但是PMO技术也有极大的缺陷，其最大的缺陷在于特殊的分子结构导致其不带有任何电荷，使得PMO无法被细胞表面的任何受体所识别，同时和无法通过转染的方式来导入细胞（RNA或者DNA通过其自身的电荷，同转染试剂如脂质体或者PEI形成复合物，来导入细胞）。因此要将PMO分子导入细胞，只有通过物理损伤细胞膜的方式来实现PMO的进入，这个缺陷极大的影响了限制了PMO作为一种可能的基因特异性药物或是敲出基因工具的研究。直到本世纪初，AVI 公司开发出了短肽连接式PMO(Peptide conjected PMO简称PPMO)才极大的推进了PMO的研究，事实上，PPMO上是在PMO分子的基础上通过共价键连接的方式连接了一段富含亮氨酸的肽段，这段肽段可以被细胞表面一种未知的受体所识别，自发性的进入细胞。不需要任何辅助方式，在体外培养实验中，只将PPMO加入培养液即可。有着极大的临床应用前景。PPMO技术的开发彻底的解决了PMO难以进入细胞的问题，但是又带了新的问题，体外实验和体内试验都证明与PMO所连接的肽段，会对机体细胞产生了毒性。但是这种毒性是如何产生的，一直是未知的。后来研究中吗，研究者发现通过在肽段的亮氨酸序列中插入其他氨基酸，来降低PPMO的毒性，但是具体机理依然不明了。目前在美国，已经有一种基于PPMO的药物开发进入的临床2期实验阶段，此外，还有更多的针对癌症，已经病毒的PPMO药物处于开发中。我们实验式目前正在做针对KSHV（Kaposi"s sarcoma-associated herpesvirus）的药物PPMO药物的研发。大概能谈一谈的也就这些，希望能算是回答了你的问题。

哦

knock out a. 敲出(击倒,打破,打落)knock down ["nu0254kdaun] 击倒,撞倒,(投篮)命中

knock down 是相对于knock out 而言的一种技术, 即通过micro RNA抑制基因的表达, 但并不能完全消除, 故认为应当翻译为"抑制"或敲低

knock down有拆卸、击倒、拒绝、降价这几个意思。[英] [nu0254k daun][美] [nɑk dau028an][例句]He died in hospital after being knocked down by a car他被车撞倒后死在了医院。knock downn.a blow that knocks the opponent off his feet同义词：knockdownv.cause to come or go down同义词：downcut downpush downpull downknock down with force同义词：deckcoldcockdumpflooradj.strong enough to knock down or overwhelm同义词：knock-down(a)powerfulA child was struck down by lightning in the storm.暴风雨中，一个小孩被雷电击倒。He manages to knock down a guard and grab a knife.他设法击倒了一个警卫，抓起了一把刀。

最爱的歌曲WakingupIseethateverythingisok睁开双眼我发现一切如期上演Thefirsttimeinmylifeandnowit"ssogreat我的生活从未如此美好呈现SlowingdownIlookaroundandIamsoamazed放慢脚步环顾四面我感到惊艳Ithinkaboutthelittlethingsthatmakelifegreat那些让生活美好的细节我开始看见Iwouldn"tchangeathingaboutit我不会让它改变Thisisthebestfeeling这感觉毫无缺陷Thisinnocenceisbrilliant这份纯真如此迷人Ihopethatitwillstay我希望它留存Thismomentisperfect完美的一瞬pleasedon"tgoaway请就此停顿Ineedyounow现在是我需要你的时候AndI"llholdontoit我会深深留住这感受don"tyouletitpassyouby别让它从你身边溜走Ifoundaplacesosafenotasingletear这片空间很安全我不再有泪眼Thefirsttimeinmylifeandnowit"ssoclear我的生活从未如此清晰呈现FeelcalmIbelongI"msohappyhere不再狂躁开始留恋这是我的乐园It"ssostrongandnowIletmyselfbesincere强烈的震撼现在我让自己变得诚虔Iwouldn"tchangeathingaboutit我不会让它改变Thisisthebestfeeling这感觉毫无缺陷Thisinnocenceisbrilliant这份纯真如此迷人Ihopethatitwillstay我希望它留存Thismomentisperfect完美的一瞬pleasedon"tgoaway请就此停顿Ineedyounow现在是我需要你的时候AndI"llholdontoit我会深深留住这感受don"tyouletitpassyouby别让它从你身边溜走It"sthestateofblissyouthinkyou"redreaming命运的眷顾让你以为这是梦幻It"sthehappinessinsidethatyou"refeeling而你真切感到幸福的浸染It"ssobeautifulitmakesyouwannacry如此美丽令你不禁泪眼潸然It"sthestateofblissyouthinkyou"redreaming命运的眷顾让你以为这是梦幻It"sthehappinessinsidethatyou"refeeling而你真切感到幸福的浸染It"ssobeautifulitmakesyouwannacry如此美丽令你不禁泪眼潸然It"ssobeautifulitmakesyouwanttocry如此美丽令你不禁泪眼潸然Thisinnocenceisbrilliant这份纯真如此灿烂itmakesyouwanttocry令你不禁泪眼潸然Thisinnocenceisbrilliance这份纯真如此珍贵pleasedon"tgoaway请不要消退CauseIneedyounow因为现在是我需要你的时候AndI"llholdontoit我会深深留住这感受don"tyouletitpassyouby别让它从你身边溜走Thisinnocenceisbrilliant这份纯真如此迷人Ihopethatitwillstay我希望它留存Thismomentisperfect完美的一瞬pleasedon"tgoaway请就此停顿Ineedyounow现在是我需要你的时候AndI"llholdontoit我会深深留住这感受don"tyouletitpassyouby别让它从你身边溜走

luciferase knockdown 荧光素酶基因敲除

knock head是英文俚语，为“呆瓜，傻瓜”意。在着英语国家中，并没有叩头这种行为。与“豆腐”，“功夫”等词的翻译同理。

歌曲名:Knock Down Dragout歌手:Weezer专辑:Weezer (Green Album)Weezer - "KNOCK DOWN DRAG OUT"Lyric By Blast Xiang.Any day now you"ll call me up on the phoneSay you love me more than you ever did beforeIt"s all that I can do right nowTo make it up to you somehowAnd meet you on the other side of homeTake no prisoners here in this knock down drag out warThat we"re waging on each other forever moreIt"s all that I can do right nowTo make it up to you somehowAnd meet you on the other side of homeSay you love me nowIt"s all that I can do right nowTo make it up to you somehowAnd meet you on the other side of homeTake no prisoners here in this knock down drag out warThat we"re waging on each other forever moreThat we"re waging on each other forever morehttp://music.baidu.com/song/1716751

古奇哪个不是那个布吉那个不是那个？

歌曲名:Nocturn歌手:Kate Bush专辑:Aerial Cd2Album:Darkness And HopeTitle:NocturnaHigh heels of crystalstuck in the heartdaylight that healsthe fullmoon scarsSpider strategyand the wish to beat one with methroughout the frightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightSister of cainall our breed slainthe youthful oathis still the samele mal de vivrethat never endsbut the hunt (for you) goes onthroughout the nightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightIt is so realSo full of light(when) Nocturna steals the nightQQ ：26090985http://music.baidu.com/song/2183056

knock off : 1,撞倒（常用简单时态） eg, please knock off your ash before it fails on the carpet . 2, 停止，中断（off 作介词用，不用被动语态） eg, when the bell rings at 12 o"clock , all the works knock off for lunch. 3, 减去，扣除（常用简单时态） eg, I"ll take both dress if you knock 10 dollars off. 4, 匆忙做完 （常用简单时态） eg, he knocked off his work in less than a month. 5, 谋杀，杀死 6，偷窃（常用简单时态） ——注意，这个phrase 在听news时常常会遇到，值得注意一下哦~！！ eg, A gang knocked off this bank yesterday. 7, 将……送到警察局 eg, The angry crowds knocked the thief off. ( 这个例句也常于新闻听力中出现， 我遇到过好几次了哦~~~） 8,击败，打垮 eg,He knocked 5 competitors and got into the final game. (注意，这个用法在听体育新闻时常遇到） 9，吃完vt. 敲落(中止) Knock it off 敲落它/终止它 Verb. 1. To make pregnant. E.g." I knocked her up and now her father wants me to marry her." 2. To awaken

-HOLD YOU kokoro wa doko e to mukau no?- tanoshiku shitete mo kanashiku naru kiete shimaitai yo nani ga hontou de nani ga uso ka ayatsurareteru kono sekai kakikake no noto ni ie nai omoi afurete iru mujaki na kokoro ga kurikaeshi sakebu koe ga boku wo yobu kara dare mo kitto hitori ja sabishii ikimono sa tsunagatte itai no sa taisetsu na kimi wo mamoru sonna negai ga ima, chikara ni kawaru yo tatoe jidai ga te to te tsunagi au koto sae tooi kioku ni shite mo boku tachi wa wakatte irukawasu omoi ga unmei sae nurikaeru koto wo -INNOCENT NOTE- hitotsu wakaru tabi hitotsu nakusu koborete yuku jiyuu nani ni mo shira nai ano hi no mama warai ae tara ii noni ne jikan sae wasurete kimi to chanto hanashi ga shitai risetto deki nai toki no ya ga mukau basho ni matsu mono wa nani? ashita ga donna forumu wo egakidashi tatte hohoen de uketomeru hitori ja tsukame nai hontou no jiyuu wa kimi to tsukuru mirai ni aru hazu -MY INNOCENT NOTE- sono egao kumoru yoru wa boku ga hikari ni kawaru yo tsutaeau kiseki wo shinjite -BELIEVE- dare mo kitto hitori ja sabishii ikimono sa tsunagatte itai no sa taisetsu na kimi wo mamoru sonna negai ga ima, chikara ni kawaru yo tatoe jidai ga te to te tsunagi au koto sae tooi kioku ni shite mo boku tachi wa wakatte irukawasu omoi ga unmei sae nurikaeru koto wo -INNOCENT NOTE-

C 试题分析：考查反意问句：主从句的反意问句，反问时，应和主句的主语保持一致。这句话和There is little doubt一致，little“几乎没有”是否定词，遵循前否后肯的原则，反意问句用is there？，你几乎不怀疑他是无辜的，是吗？选C。点评：主从句的反意问句，反问时，应和主句的主语保持一致。但当主句是I think，I suppose，I believe，I imagine等时，反意问句是和从句决定。如果是否定前移，反意问句是肯定。

中文：作凹痕于是澳大利亚的品牌，UGGNOCK受到很多年轻人的喜爱，耐穿不说风格多样，穿在脚上不会磨脚而且很好搭配

是澳大利亚的品牌，1979年，冲浪运动员布莱恩·史密斯（Brian Smith）买了一些澳大利亚生产的羊皮靴子带到美国，开始在纽约出售。主要为加利福尼亚的冲浪者使用。后来他建立了UGG Holding公司，注册了UGG商标，但是由于经营不善，1995年，布莱恩·史密斯将股份卖给Deckers（德克斯）户外用品有限公司运营，该公司使好莱坞明星穿着而走红美国，进而在多个国家获得认可。扩展资料：UGGNOCK现代营销学之父科特勒在《市场营销学》中的定义，品牌是销售者向购买者长期提供的一组特定的特点、利益和服务。品牌是给拥有者带来溢价、产生增值的一种无形的资产，它的载体是用于和其他竞争者的产品或劳务相区分的名称、术语、象征、记号或者设计及其组合，增值的源泉来自于消费者心智中形成的关于其载体的印象。品牌承载的更多是一部分人对其产品以及服务的认可，是一种品牌商与顾客购买行为间相互磨合衍生出的产物。参考资料来源：百度百科-UGG

是澳大利亚的品牌，UGGNOCK受到很多年轻人的喜爱，耐穿不说风格多样，穿在脚上不会磨脚而且很好搭配。

uac00uc218: uc720ubbf8(Youme)uace1uba85: Knockuc568ubc94: Melody & Memoryub611ub611ub611 ub208ubb3cuc774 ub098uc694 uc5b4ub514uc11c ubb34uc5c7uc744 ud558ub098uc694 ub2e4uc2dcub294 uc0acub791 uac19uc740 uac70 ud560 uc218 uc5c6uac8c ud574ub193uace0 ub611ub611ub611 ube44uac00 ub0b4ub824uc694 uba4dud558ub2c8 ucc3d ubc16uc744 ubcf4ub2e4uac00 uc6b8ucee5ud574uc838 uc798 uc9c0ub0b4ub294uc9c0 uad1cud788 ud63cuc790 uac71uc815ud558ub124uc694 uadf8 ub9c8uc74cub3c4 ubaa8ub974uba74uc11c ub09c uc544ud504uac8cub9cc ud588uc5b4uc694 uc788ub294 uadf8ub300ub85c uc544ubb34 uacc4uc0b0 uc5c6ub294 uc0acub791uc744 ubab0ub77cubcf8 uac70uc5d0uc694 uace0ub9c8uc6e0uc5b4uc694 ud589ubcf5ud588uc5b4uc694 uc0acub791uc774ub780 uac10uc815uc744 uc54cuac8c ud574uc918uc11c ub9ccub098uc57c ud574uc694 ub9d0ud574uc57c ud574uc694 uc9c4uc2ecuc73cub85c uadf8ub300 uc55euc5d0uc11c uc6a9uc124 ube4cuc5b4uc57c ud574uc694 ub611ub611ub611 uc0c8ubcbduc774 uc640uc694 ubc24uc0c8uc6cc uadf8ub9acuc6ccud558ub2e4uac00 uc6b8ub2e4 uc9c0uccd0 uc7a0uc774 ub4e4uc5c8uc8e0 ub0b4uc77cuc774uba74 uad1cucc2euc744uae4cuc694 ub09c uc6b0uc5f0ud788 ub9ccub098uae30ub97c ub298 uc6d0ud588ub294uc9c0 ubab0ub77cuc694 uadf8ub300 uc88buc544ud55c uba38ub9acuc870ucc28 ubc14uafb8uc9c0 ubabbud574 uadf8ub300ub85c uc788uc5b4uc694 uace0ub9c8uc6e0uc5b4uc694 ud589ubcf5ud588uc5b4uc694 uc0acub791uc774ub780 uac10uc815uc744 uc54cuac8c ud574uc918uc11c ub9ccub098uc57c ud574uc694 ub9d0ud574uc57c ud574uc694 uc9c4uc2ecuc73cub85c uadf8ub300 uc55euc5d0uc11c uc6a9uc124 ube4cuc5b4uc57c ud574uc694 ub05dub09c uac74uac00uc694 ucd94uc5b5uc778uac00uc694 ubbf8uc6ccud560 uc218ub3c4 uc5c6uac8c uc798ud574uc918 ub193uace0 uc798ubabbud588uc5b4uc694 ubbf8uc548ud588uc5b4uc694 ud3c9uc0dd ud6c4ud68cud558uac8c ub418uaca0uc8e0 uadf8ub300 ub193uce58uac8c ub41cuac78

racism是一个名词，种族歧视的意思，ethnocentrism也是名词，民族优越感的意思

Triple 8 - KnockoutGirl I"ve been thinkin "boutHow you have let me downFill my head with your liesThink you gettin one byChecking him when you"re with meThinkin" that I don"t seeLittle games that you play Make me wanna walk awayYou come over late at night (you come over late at night)Sayin everything is rightBut my boys keep tellin meTake her out, take her out(take her out)My boys keep sweatin meI"m stressin out, stressin outI don"t really understand (I don"t really understand)What kinda man you think I amHow I could be that kindaHow I could be that kinda manChorus:I wanna knockout(You know I wanna out the light, I wanna)Knockout(You know I wanna start a fight, I wanna)Knockout(You know I wanna do this right)Cuz you"re stressin me,Messin me,I won"t fall that easilyI wanna knockout(You know I wanna let it go, I wanna)Knockout(Do you like you did me before, I wanna)Knockout(Be the one that"s opening the door)Time for me to say goodbyeGot to get you out my lifeEverytime that you creepI"m not home fast asleepGot my spies right in placeThey even told me his nameWhy you keep frontin whenYou know I know the gameI can feel it all aroundGirl you"re "bout to go downYou come over late at night (you come over late at nigh)Sayin everything is right (yeah yeah)But my boys keep tellin me Take her out, take her out (take her out)My boys keep sweatin meI"m stressin out, stressin out (stressin me out)I don"t really understand What kinda man you think I amHow I could be that KindaHow I could be that kinda manChorus 3x

[Verse 1]Girl I"ve been thinking "boutHow you have let me downFill my head with your liesThink you getting one byCheckin him when your with meThinkin that I don"t seeLittle games that you playMake me wanna walk away[B Section 1]You come on over late at night(You come on over late at night)Sayin everything is rightBut my boys keep telling meTake her out, ,take her out (Take her out)My boys keep sweatin meI"m stressing out, stressing outI don"t really understand(I don"t really understand)What kinda man you think I amHow I can be that kindaHow I can be that kinda man[Chorus]Knockout(You know I wanna out the light, I wanna)Knockout(You know I wanna start a fight, I wanna)Knockout(You know I wanna do this right)Cos ya stressin me messin meI won"t fall so easilyI wanna Knock out(You know I wanna let it go, I wanna)KnockoutDo you like you did me before, I wannaKnockoutBe the one that"s opening the doorTime for me to say good-byeGot to get you out my life[Verse 2]Every time that you creepI"m not home fast asleepGot my spies right in placeThey even told me his nameWhy you keep frontin whenYou know I know the gameI can feel it all aroundGirl your "bout to go down [B Section 2]You come on over late at night(You come on over late at night)Sayin" everything is rightYeah yeahBut my boys keep tellin" meTake her out, take her out(Take her out)My boys keep sweatin meI"m stressin" out, take her out(Stressing me out)I don"

歌名：knockinI"m lost in a world that rattles my brainI"m cleaning up my life from the mess you madeoh, oh. Whoa, oh, oh, ohMy soul"s in debt, but my bills are paidI"d give anything just to make an escapeoh, oh. whoa, oh, oh, ohSo you can keep Knocking, Knocking, KnockingBaby, you"re rockin" But there ain"t no way I"m ever letting you inNot againso keep on Knocking, Knocking, Knockingbut, baby, you"re better off walking"Cause I ain"t gonna let you inNever again, no, not againso, so long honey there"s not a blame, we"ve lived in the dream that face"d fadeoh, oh. whoa, oh, oh, ohAnd no scares when guy"s wasteland to say, dream poor my heart break for payoh, oh. whoa, oh, oh, ohSo you can keep Knocking, Knocking, KnockingBaby, you"re rockin" But there ain"t no way I"m ever letting you inNot againso keep on Knocking, Knocking, Knockingbut, baby, you"re better off walking"Cause I ain"t gonna let you inNever againI went out on a limb againI guess i had to lose to winI was too confused to know which way to turnAnd she could be a milliomaire. be a mad I don"t care"Cause, baby, there"s nobody homeNobody"s home, yeahSo you can keep Knocking, Knocking, KnockingBaby, you"re rockin" But there ain"t no way I"m ever letting you inNot againso keep on Knocking, Knocking, Knockingbut, baby, you"re better off walking"Cause I ain"t gonna let you inNever again, no, not again wistand

摇滚

knock off: vt. 敲落(中止)knock out: 敲出(击倒,打破,打落)

knockdown1.打倒，击倒；撞倒；击落：Theangrymanknockedhimdown.那个愤怒无比的人将他击倒了。Icarelesslyknockedthechairdown.我不小心将椅子撞倒了。2.毁掉，拆毁，破坏：It"sapitythattheoldhousewasknockeddown.很可惜，那幢老房子被拆毁了。3.拆卸；拆散(以便于装运)：Themachinehasbeenknockeddownreadyforshipment.机器已拆好准备装船。

一个男人在十月22号发生一次侥幸的撞击

歌曲名:Knock On My Door (Live Version)歌手:Fanny专辑:First Time In A Long Time: The Reprise RecordingsBarbie, you are friend of mine.Hate to be the one to break the news.That girl you wanna marry, she has been lying down.You don"t see she has been using you.You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymoreBarbie, open up to me.Talk to me, tell me what you feel.Let me in the doorway, show your broken heart,let me be the one to have you healed.You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymore献给喜欢这首歌的人You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymoreYou don"t have to hurt anymoreYou don"t have to hurtanymoreen··············http://music.baidu.com/song/8225386

歌曲名:Knock On My Door歌手:Rebecca Blaylock专辑:TV Magic ⅡBarbie, you are friend of mine.Hate to be the one to break the news.That girl you wanna marry, she has been lying down.You don"t see she has been using you.You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymoreBarbie, open up to me.Talk to me, tell me what you feel.Let me in the doorway, show your broken heart,let me be the one to have you healed.You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymore献给喜欢这首歌的人You can always cry on my shoulder.You can always knock on my door.I will always be here, whenever you come calling.You don"t have to hurt anymoreYou don"t have to hurt anymoreYou don"t have to hurtanymoreen··············http://music.baidu.com/song/59141740

意思是：连锁反应

Knock on: 敲（门、窗等） 例句与用法: 1. He accidentally knocked on (the pass from Jones). 他非故意犯规, 用手向前击了（从琼斯传来的）球. 2. Jim sat up when he heard the knock on his bedroom door. 吉姆听到敲寝室门的声音时便坐了起来。 3. Please do not enter before knocking on the door. 进来前请先敲门。 Knock at: vt. 敲 例句与用法: 1. There was a furious knock at the door. 有人在猛烈地敲门。 2. Did I hear a knock at the door? 是有人敲门吗? 3. Did you hear the knocks at the door? 你听到敲门声了吗？

敲门声

knock是一个英文单词，名词、动词，作名词时意思是“敲；敲打；爆震声”，作及物动词时意思是“敲；打；敲击；批评”，作不及物动词时意思是“敲；打；敲击”。 knock常用短语 1、knock...off 把...撞倒 2、knock out 击倒;打昏 3、knock down 击倒;撞倒;拆除。 4、knock up 匆忙做完;使...精疲力尽 5、knock it off 别再讲下去 knock常用例句 1、Knock on the door still so rhythmic continue. 敲门声照旧那样有节拍地持续。 Then there came a knock at the door. 接着听到了敲门的声音。 2、My health compels me to knock off work. 我的健康情况迫使我停止工作。 3、He manages to knock down a guardandgrab a knife. 他设法击倒了一个警卫，抓起了一把刀。

knock on 敲(门、窗等);撞knock at 敲(门,窗等)当knock作为名词的时候,介词用at,如a knock at the door 敲门声 动词是介词用on,如knock on the door 敲门Knock on the door before you enter.进屋之前要先敲门。When I fell I got a terrible knock on the head.我摔倒时头撞得很厉害。He got a nasty knock on the head.他头部遭到重重一击。Did I hear a knock at the door?是有人敲门吗?I was wakened by a knock at the door.敲门声把我吵醒了希望对你有帮助，满意请及时采纳，你的采纳是我回答的动力！

Knock on: 敲（门、窗等） 例句与用法: 1. He accidentally knocked on (the pass from Jones). 他非故意犯规, 用手向前击了（从琼斯传来的）球. 2. Jim sat up when he heard the knock on his bedroom door. 吉姆听到敲寝室门的声音时便坐了起来。 3. Please do not enter before knocking on the door. 进来前请先敲门。 Knock at: vt. 敲 例句与用法: 1. There was a furious knock at the door. 有人在猛烈地敲门。 2. Did I hear a knock at the door? 是有人敲门吗? 3. Did you hear the knocks at the door? 你听到敲门声了吗？

knock at 敲(门、窗等)，knock off 击倒;停工;中断，knock down 击倒;拆卸，knock at the door 敲门;把……撞倒;撞墙，knock out 敲空;击倒;打破;使筋疲力竭，knock it off 停止做;住口,别再讲下去了，knock on the door 敲门，knock over 打翻;撞倒，knock in (把钉子)钉入，knock on wood 敲木头。

knock on 敲(门、窗等);撞knock at 敲(门,窗等)当knock作为名词的时候,介词用at,如a knock at the door 敲门声 动词是介词用on,如knock on the door 敲门Knock on the door before you enter.进屋之前要先敲门。When I fell I got a terrible knock on the head.我摔倒时头撞得很厉害。He got a nasty knock on the head.他头部遭到重重一击。Did I hear a knock at the door?是有人敲门吗?I was wakened by a knock at the door.敲门声把我吵醒了希望对你有帮助，满意请及时采纳，你的采纳是我回答的动力！