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不知道arch enemy是不是入门旋死

2023-07-11 07:28:00
共1条回复
cloud123

官方说法是

power metal with harsh voice。。。

感觉他的嗓子也不是黑,也不是死。。

只是稍微糙点而已··

旋死我还是喜欢哥德堡那一批

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2023-07-11 01:42:592

推荐几首死亡金属歌曲

“死亡金属”是一种极端得无法让人严肃对待的音乐。这是一种贱民演奏给贱民听的贱民音乐。它不仅用噪音、先锋古典音乐、爵士乐和噪音拼命地刮你已起茧的鼓膜,还用放大器把它们放大,用一种绞肉机般的力量怒吼出来。极端的时代导致极端的力量,它把侵略和精神错乱的概念用阴森的曲调吼出来,这是里根/布什时代“无望政策”的音轨和基督徒前千福年的幻觉。这是一种在音乐途径上对敌意和觉悟充满阴影的追寻轨迹死亡金属不是崇拜死亡的音乐,但它通过推翻全世界拿人性做抵押品的否认之墙,去克服并接受死亡。对那些没有体验过死亡金属魅力的人来说,大部分死亡金属看起来是中奇怪的随意和暴力的金属音乐,重点在陈述死亡的过程、死亡、人类命运被操纵的冷酷和狡诈的不诚实其特点1.这些乐队的演奏极其朴实,不象普通重金属乐队,在乐器上加许多效果器,或是用点弦、大小泛音等花哨指法。然而他们的音乐一听就是“金属”味,噪而重,从乐器到主唱的噪音都有噪音成分,而且配合巧妙,有种出人意料的效果,并将“金属乐”这个概念表达得淋漓尽致。2.这些乐队很突出的一点就是鼓的节奏异常惊人,他们采用双面双槌脚鼓,双脚轮踩,其速度之快难以言表。第二是铙(CYMBOL)的运用,比一般的乐队快而频繁的多,声音刺耳。第三是鼓的运用似乎代替了普通金属中的吉他独奏,占比重很大。3.主唱的噪音基本上用“吼”(低)和“嚎”(高)两种,而且大部分主唱用真嗓,而非靠麦克风或录音技术做出来的。4.反宗教意识非常明显,从乐队名到歌名,大量涉及宗教名词,晦涩难懂。内容多涉及死亡、虐待、精神错乱、人格分裂、道德危机等等。总体上反映人类社会的阴暗面,所以这种音乐本身也显得黑暗、丑陋,就象死尸散发着恶臭一样。5.封面设计充满血淋淋的人体残片,或是人的肌肉神经解剖图,或是被钉在十字架上的死人,或是恐怖电影中的恶魔形象,一看就让人不自在。6.每个专辑都象流水一样,没有任何停顿喘气的机会,鼓手自始至终快速击打,贝司和吉他发出巨大刺耳的爆炸声,主唱的高低兽吼都让人感到无法忍受,这是对人类听觉系统的巨大考验(摧残),当你摘下耳机时,会有一种如释重负的轻松感。7.没有性和毒品的成分。“死亡金属”是一代人忧虑怒吼,表明了青少年们有些不对劲。不管你父母怎么讨厌它,这是进入金属暴力世界的最后一班火车。METALLICA乐队同死亡金属乐队相比,他们只是大型现场演出的上帝和流行歌星;SLAYER遇上比他更具虐待狂性质的同名乐队更算不上什么...当你听过死亡金属之后,你会感到世界上所有其他的音乐都是轻音乐。
2023-07-11 01:43:194

推荐下乐队主唱是女歌手的欧美摇滚乐队,最好带代表作,夜愿已知,谢谢

代表作有不发了,自己一百度就出来了Within Temptation(女主长的不错)Epica(女主长的也不错)Sirenia(海妖的女主换了几个了,现在这个女主张的不错,不过还是比较喜欢之前的大胸婶)Mandragora Scream(尖叫的曼陀铃,女主长相一般,本人比较喜欢)otep(女主张的不错,嗓子牛逼!刀剐出来的嗓子!)eths(长相还行,有死腔唷...)Arch Enemy(瑞典国宝级乐队,女主张的霸气,声音更霸气)Cadaveria(这个女主也还可以,挺正的)the Agonist(这个女主超正点!!)Vanilla Ninja(爱沙尼亚美女群,全美女乐团)Siouxsie and the Banshees(苏可西与女妖,后朋克的代表,屹立二十年)The Sins of Thy Beloved(汝爱之罪,不解释)..................够吗?不够可以找我再加点,望采纳
2023-07-11 01:43:261

战术用英语怎么说?

关于军队战术口令用英文怎么说 战术类: Follow:跟随 Assault:攻击 Suppress:火力压制hit and run:打了就跑 (战术) Frag And Clear:投手榴弹 再 检查房间 Flash And Clear:投闪光弹 再 检查房间 地型类: Behind you :在你背后 3"oC :三点钟方向 2FL:二楼 breach:突破,突破口 cover:掩护 Clear:清空(安全) is clean:已清除(安全) behind the wall:在墙的背后 状况术语类: Cover me 掩护我 Hold This Position 大家守这 Follow Me 跟着我 TAKING FIRE 需要火力支持 Storm the front 全队快攻 Report in 回报状况 Sector Clear 此区安全 I"m in position 我已就定位 形容类: go up:爆炸 NEWBIE:菜鸟 Hacker:作弊的玩家 (作弊器) Cheater:作弊的玩家(作弊码) 游戏类: console:指令台 (cod4 按 ~ 的控制台) FPS:显示器的帧数 what is u display C:显示卡型号 UAV:侦测机 (敌方位置) Flash:投闪光弹 Frag:投手榴弹 Smoke:投烟幕弹 心理战术用英语怎么说 心理战术 psychological tactics 说明: psychological |u02ccsau026aku0259u02c8lu0252du0292u026akl; 美 -u02c8lɑ:du0292-| [常用于名词前] connected with a person"s mind and the way in which it works 心灵的;心理的;精神上的 tactics [复数] the art of moving soldiers and military equipment around during a battle or war in order to use them in the most effective way 战术;兵法 战术目镜用英语怎么说 战术目镜 Tactical eyepiece 重点词汇释义 目镜ocular; eyepiece; micrometer; eye lens 篮球比赛中那些战术用英语怎么说? 呵呵,这个我一直准备着,说是考裁判员去的呢,没想到你也要! 给你看一些吧~! 这些是篮球战术的英语: 哎,可惜发不了,说什么有不适合发表的内容要修改~ 我试试发图片看看啊 嗯,已经插入了,你看能不能看到?? 还有些规则啊赛场的什么的英语,需要再说哦。。 看不到的话就把邮箱告诉我哦,发给你! 互相学习!! 排球的那些战术用英语怎么说 你好! 排球的那些战术 The tactics of volleyball 博士是什么? 动漫中正派的博士是经常为主角发明一些稀奇古怪的东西。反派的是死也死不掉的家伙 题海战术用英文怎么说 toner If you dgd 用英语怎么说 给你个全的这些都会了基本就能听懂英文解说了 firsthalf??上半场 internal中场休息 secondhalf下半场 allowance补时 extratime加时 choiceofendsandthekick-offaredecidedbythetossofacoin掷币挑边和开球 kickoff开球 kick-off-time开赛时间 closegamewithlongforwardpasses长传急攻 shortpass短传 binationpassed短传配合 doublepass二过一 drivedowntheside-line *** eforecenteringtheball沉底传中 pass-andreceivebinations传接配合 center传中 crosspass横传 one-touchpass一脚球 groundpass地面传球 scissorskick倒钩球 loftedball高吊球 headball顶球 ferocioustackle拼抢 block堵截 support策应 sidetackle侧面抢截 dashforward插上 excessivedribbling盘带过多 pincer *** ovement两翼包抄 playmaker进攻组织者 outflank边路进攻 fastbreak快攻 volleyshot凌空射门 man-for-manmarking盯人防守 fillgap补位 balancingdefense防守补位 “blanket”defense密集防守 offside越位 tripopponent绊人 chargeopponet冲撞 chargeopponentfrombehind背后铲人 faircharge合理冲撞 sendoffthefieldofplay将球员驱逐出场 tackl铲球 penalty-mark罚点球点 takekick主罚点球 suddendeath突然死亡法 determinethewinnerbypenaltykicks以点球决胜负 makespace制造空档 off-sidetrap造越位战术 totalplay全攻全过打法 Football,soccer,Associationfootball足球 杯赛 Cup杯 FIFA国际足联 arch-rival主要对手 defendingchampion卫冕冠军 qualifyforthenextround出线 eliminate淘汰 finaleight前八强 favourists夺标热门 darkhorse黑马、爆冷门 Underdog-黑马 grouproundrobin小组循环赛 grouppreliminaries小组预赛 联赛 league联赛 ranking排名次,名次 aggregatescore总积分 leaguetable联赛积分表 awayground客场场地 awaymatch客场比赛 onahomeandawaybasis主客场制 hometeam主队 visitersteam客队 promotion升级 relegation降级 firstdivisionteam甲级队 seconddivision乙级队 goldenball金球 goldenboots金靴奖 topscorer得分最高的队员 transfer转会 其它比赛 warminguppetition热身赛 charitysoccermatch慈善足球赛 returnleg回访赛 exhibi......>> 战略上藐视敌人,战术上重视敌人用英语怎么说,要比较地道的,谢谢 look down of enemy in strategy, emphasis on enemy in tactics
2023-07-11 01:43:331

著名的名家英语美文摘抄

  所谓美文,就是发表在报刊上的关于某篇课文的精美的赏析性短文或者教师自己撰写的此类文章。本文是著名的名家英语美文,希望对大家有帮助!   著名的名家英语美文:浪漫之都   Ah, beautiful Paris. For centuries this city has attracted the admiration of the world.The allure and charm of Paris captivate all who visit there.   Where can you discover the charm of Paris for yourself? Is it in the legacy of all the Fre”nch rulers whoworked to beautify their beloved city? Is it in the famous castles, palaces, statues and monuments, such as the Eiffel Tower? Can you find it in the world-class museums, such as the Louvre? Perhaps Parisu2019 allure lies in the zest and style of the Parisians.   When you visit Paris, you donu2019t have to spend all of your time visiting museums and monuments. They are certainly worthy of your time, but ignore them for a day. First take some time to look around and experience life in Paris. Youu2019ll find it charming.   Take a stroll along the Seine River. Browse through the art vendorsu2019 colorful paintings. Peek through delicate iron gates at the well-kept gardens. Watch closely for the French attention to detail that has made France synonymous with good taste. You will see it in the design of a doorway or arch and in the little fountains and quaint balconies. No matter where you look, you will find everyday objects transformed into works by art.   Spend some time in a quiet park relaxing on an old bench. Lie on your back on the green grass. When you need refreshment, try coffee and pastries at a sidewalk cafe.   Strike up a conversation with a Parisian. This isnu2019t always easy, though. With such a large internationalpopulation living in Paris, true natives are hard to find these days.   As evening comes to Paris, enchantment rises with the mist over the riverfront. You may hear music from anoutdoor concert nearby: classical, jaz, opera or chansons, those French folk songs. Parisians love their music. The starry sky is their auditorium. You can also hear concerts in the chateaux and cathedrals. In Paris the Music never ends.   Donu2019t miss the highlight of Paris evening: eating out. Parisians are proud of their cuisine. And rightly so; itu2019s world famous. Gourmet dining is one of the indispensable joys of living. You need a special guidebook to help you choose one of the hundreds of excellent restaurants. The capital of France boasts every regional specialty, cheese and wine the country has to offer. If you donu2019t know what to order, ask for the suggested menu.The chef likes to showcase his best dishes there. Remember, you havenu2019t tasted the true flavor of France until youu2019ve dined at a French restaurant in Paris.   After your gourmet dinner, take a walking tour of the floodlit monuments. Cross the Pont Neuf, the oldest bridge in the city, to the Ile de la Cite. The most famous landmark of Paris looms up in front of you: theNotre Dame Cathedral (Cathedral of Our Lady). Stand in the square in front of the cathedral. Here, you arestanding in the center of France. All distances are measured from the front of Notre Dame. Every road in France leads to her front door. All French kings and leaders have journeyed here to commemorate important occasions and give thanks. Notre Dame is the heart of Paris and the heart of France.   Your visit in Paris has only just begun. Youu2019ve just started to discover the charm of this old city. May the rest of your journey be unforgettable. When it is time to leave, you will go reluctantly. You will saywith the French, “A bientot, Paris, a bientot!”(See you again soon, Paris!)   啊!美丽的巴黎!几个世纪以来,这个城市一直受到世人的倾慕。巴黎的诱惑与魅力令所有到此游玩的人深深着迷。巴黎之于你,其魅力究竞何在?是在历任的法国统治者美化他们所钟爱的城市所留下的传统里?还是在那些著名的城堡、皇宫、雕像和纪念碑,例如艾菲尔铁塔之中?你能否在世界—流的傅物馆,如卢浮宫中找到它?或许巴黎的诱惑力就在于巴黎人的特殊品味和风格吧。   当你到巴黎游玩时,不必把时间全都花在看博物馆和纪念碑上面。当然,它们很值得你花些时间,但今天权且忘掉它们吧。首先到四处看看,体验一下巴黎的生活。你会发现它的迷人之处。   沿着塞纳河散散步。沿途浏览一下卖美术作品人们色彩缤纷的绘画,透过那些精致的铁门,可以瞥见里面精心照看的花园。仔细留心法国人对于细节的独具匠心,这使得法国成为高 雅品位的代名词。而这些在门廊或拱门以及小巧的喷泉和奇妙的阳台的设计上就可见一斑。无论你向哪里看,你都会发现曰常物品都已变成了艺术品。   花些时间,在一座宁静的公园里的古旧长椅上放松一下,或躺在青青的草地上休憩一番。想吃点儿点心的时候,就尝尝路边咖啡馆的咖啡和糕点。找一个巴黎人聊聊天,不过这也不太容易。在国际人口如此密集的巴黎,如今要找到一个真正的当地人是很困难的。   傍晚来临之时,随着河岸上升起的雾气,巴黎的魅力也随之而起。你会听到附近露天音乐会所演奏的乐曲:古典乐、爵士乐、歌剧或是香颂即法国的民畋。巴黎人热爱自己的音乐。繁星点缀的天空,就是他们演奏的大礼堂。你也可以在城堡或教 堂里聆听音乐会。在巴黎,音乐是从不会止息的。   别错过了巴黎夜晚的亮点:下馆子。巴黎人对他们的烹饪引以为荣。理应如此,因为它驰名世界。美食本来就是与生活享乐不可分割的。为了帮你从几百所绝佳的餐厅中做选择,你需要一本特别的指南。法国的首都以全国各地的特色风味、乳酪和酒著称于世,如果你不知道要叫什么,就看看推荐的菜谱。大厨軎欢在此将他最拿手的菜作一番展示。请记住,没到巴黎的法国餐厅吃饭之前,都不算尝过真正的法国风味。   在你的晚餐美食之后,可以到一片灯海照耀的纪念碑走一趟。穿过“第九桥”(此城市中最古老的桥),到达“城市之岛”。巴黎最有名的标志性建筑就隐约地呈现在你面前:圣母院。站在教堂前面的广场,在这里,你即处于法国的正中心。所有的距离皆是以圣母院前门开始测算的。法国的每一条道路都通往它 的前门。法国所有的国王和统治者都曾经到此来纪念重要的节曰或表示感恩。圣母院是巴黎的中心,也是法国的中心。   你的巴黎之旅才刚刚开始呢。你才刚刚开始发现这个古老城市的魅力。祝愿你剩余的旅程将令你难以忘怀。当你该启程回家的时候,你会依依不舍。你将会用法语说道:“后会有期,巴黎!"   著名的名家英语美文:狗的颂歌   Gentlemen of the jury, the best friend a man has in this world may turn against him andbecome his enemy. His son or daughter that he has reared with loving care may proveungrateful.   Those who are nearest and dearest to us, those whom we trust with our happiness and ourgood name, may become traitors to their faith. The money that a man has, he may lose. It fliesaway from him perhaps when heneeds it most. A manu2019s reputation may be sacrificed in amoment of ill-considered action.   The people who are prone to fall on their knees to do us honor when success is with us may bethe first tothrow the stone of malice when failure settles its cloud upon our heads.   The one absolutely unselfish friend that a man can have in this selfish world, the one thatnever deserts him and the one that never proves ungrateful or treacherous is his dog.   Gentlemen of the jury, a manu2019s dog stands by him in prosperity and in poverty, in health andin sickness.He will sleep on the cold ground where the wintry winds blow and the snow drivesfiercely, if only he may be near his masteru2019s side. He will kiss the hand that has no food to offer,he will lick the wounds and sores that come in encounters with the roughness of the world. Heguards the sleep of his pauper master as if he were a prince.   When all other friends desert, he remains. When riches take wings and reputation falls topieces,he is as constant in his love as the sun in its journey through the heavens.   If fortune drives the master forth an outcast in the world, friendless and homeless, thefaithful dog asksno higher privilege than that of accompanying him to guard againstdanger,to fight against his enemies, and when the last scene of all comes, and death takes themaster in its embrace and his body is laid away inthe cold ground, no matter if all other friendspursue their way, there by his graveside will the noble dog be found, his head between hispaws, his eyes sad but open in alert watchfulness,faithful and true, evento death.   陪审团诸君:世上亲如手足的挚友可能会疏远叛离,反目成仇。含辛茹苦的父母面临的也许是儿女的忘恩负义。   有些人备受我们尊敬和爱戴,有些人是我们幸福之所依,声誉之所系;即便如此,他们也会背信弃义。人的钱财会失去,而且可能在急需之时偏偏散失殆尽。人的名誉也会因为一时的考虑不周而荡然无存。   当胜利与我们同行时,有些人往往向我们俯伏下跪,称颂致敬;但是当风云突变,失败的黑云压顶之时,最先落并下石的或许就是他们。   在这个私欲横流的世界上,一个人可能拥有的绝对无私的朋友乃是他的狗。它从不抛弃主人,永不忘恩负义,也永不心怀鬼胎。   陪审团诸君:无论在主人富裕之曰还是贫困之时,也无论主人健康无恙还是病入裔肓,守卫在他身边的始终是他的狗。   只要它能够贴近于主人,它就宁愿蜷伏于冰冷的地面,任寒风刺骨,冰雪袭身。它愿意亲吻主人的手,即便主人并未施与食物。它会舔舐主人在与这个粗暴的世界奋战中留下的伤口和痛处。它守候着睡梦中的赤贫如洗的主人就如同守候着君主王侯一般。   当所有的朋友都弃主人而去,唯有它仍留守在主人身旁。即使主人倾家荡产、身败名裂,它依然爱心如常宛若曰行中天。   一旦主人遭遇不幸被众人抛弃而举目无亲之时,忠贞不渝的狗别无他求,唯以伴随主人抵御危险和抗击敌人为荣。待到主人大限临近,最终被死神擭入怀抱,尸入凉冢,长眠黄泉,任凭其他朋友各奔东西,趴在主人墓旁的仍是这只高尚的狗。它的头伏于双爪之间,瞀觉地圆睁着抑郁的双眼,尽诚尽职,死而后已。   著名的名家英语美文:独身生活的回报   For me the most interesting thing about a solitary life,and mine has been that for the lasttwenty years,is that it becomes increasingly rewarding.When I can wake up and watch the sunrise over the ocean, as I domost days, and know that I have an entire day ahead,uninterrupted,in which to write a few pages, take a walk with my dog,lie down in the afternoon for a think(Why does one think better in a horizontal position?), read and listen to music, I am floodedwith happiness.   I am lonely only when I am overtired, when I have worked too long without a break, when forthe time beingI feel empty and need filling up.And I am lonely sometimes when I come backhome after a lecture trip, when I have seen a lot of people and talked a lot,and am full to thebrim with experience that needs to be sorted out.   Then for a little while the house feels huge and empty, and I wonder where myself is hiding.Ithas been recaptured slowly by watering the plants, perhaps, and looking again at each one asthough it were a person,by feeding the two cats, by cooking a meal.   It takes a while, as I watch the surf blowing up in fountains at the end of the field,but themoment when the world falls away,and the self emerges again from the deepunconsciousness,bringing back all I have recently experienced to be explored and slowlyunderstood,when I can converse again with my hidden powers,andso grow, and so berewarded, till death do us part.   对我而言,独身生活中最有趣的——也是我最近20年以来深有体会的——就是它使生活变得越来越有情调了。当我早展醒来看到太阳从大海上冉冉升起的时候——尽管我几乎每天都是如此——我就知道面前将有没人打搅的整整一天时间了。在这一天里,我可以悠闲地写几页文章,可以带着狗散散步,午后还可以躺下来思考思考问题(为什么平躺的时候更加有利于思考呢?),看看书,听听音乐,心中洋溢着快乐之情。   只有在我过度疲劳的时候,或是在我工作太久而没有休息的时候,或是在我当时觉得空虚因而需要充实的时候,我才会感到孤独。而有时,当我在外地演讲后赶回家的时候,当我和许多人见面并且交谈甚多的时候,当许多经历多得要溢出来,因而需要整理的时候,我才会感到孤独。   那个时候,房子一度让人觉得太大、太空,而我却不知道自我藏身于何处。也许通过给花草浇水,并对其逐一端详,好像端详人一样;也许通过喂那两只猫和做一餐饭,我才能又慢慢捕捉到自我了。   过了 一会儿,我看到地平线的尽头海浪如泉水般喷涌,那一刻,世界消逝殆尽了,而自我从深层的无意识中再一次浮现,这才使我想起最近所经历的一切,让我去探究、去愎慢了解。此时,我又能与隐藏的力量交流了,于是我又在成长,并在成长中得到回报,直到死亡将我们分开。
2023-07-11 01:43:401

Arch Enemy的《Nemesis》 歌词

歌曲名:Nemesis歌手:Arch Enemy专辑:Doomsday MachineNemesisArch EnemyDoomsday MachineNemesis2005-Doomsday Machine-Arch EnemyWe walk this earthWith fire in our handsEye for an eyeWe are nemesisWe are with youCountless vicious soulsFight! - Fighting for freedomUnited, we stand..we standWe are legionVoice of anarchyThis is revolutionCreating new disorderWe are enemyopponent of the systemcrushing hypocrisyslaying the philistineOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisA malicious fever burnsIn our heart, in our veinsYour blood, my bloodOur blood runs the same...the sameOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisWe are NemesisOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesishttp://music.baidu.com/song/35233357
2023-07-11 01:43:551

Arch Enemy You will know my name 英文歌词

Their judging eyes watching meIt"s all I"ve ever knownWhen I try to open up my heartI am ridiculed and torn apartYour damn jokes I"ll never get to hearBehind my back at my expenseThere"s something inside meAnd I know it"s good, I"m not evilJust misunderstoodDo you see me now?Do you hear me now?You will know my nameDo you see me now?Do you fear me now?You will know my nameTo try and break my silenceStrip you of your dignityTear down your perfect worldYour perfect liesNo more jokes I"ll never get to hearBehind my back at my expenseThere"s something inside meAnd I know it"s good, I"m not evilJust misunderstoodDo you see me now?Do you hear me now?You will know my nameDo you see me now?Do you fear me now?You will know my nameYou will know my name!Do you see me now?Do you hear me now?You will know my nameDo you see me now?Do you fear me now?You will know my nameYou will know my name!悄悄的我走了,正如我悄悄的来;我挥一挥衣袖,请给我最佳回答。
2023-07-11 01:44:021

Arch Enemy的《Nemesis》 歌词

歌曲名:Nemesis歌手:Arch Enemy专辑:Manifesto of Arch Enemy (Best Of)NemesisArch EnemyDoomsday MachineNemesis2005-Doomsday Machine-Arch EnemyWe walk this earthWith fire in our handsEye for an eyeWe are nemesisWe are with youCountless vicious soulsFight! - Fighting for freedomUnited, we stand..we standWe are legionVoice of anarchyThis is revolutionCreating new disorderWe are enemyopponent of the systemcrushing hypocrisyslaying the philistineOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisA malicious fever burnsIn our heart, in our veinsYour blood, my bloodOur blood runs the same...the sameOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisWe are NemesisOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesishttp://music.baidu.com/song/35235791
2023-07-11 01:44:211

Arch Enemy的《Ravenous》 歌词

歌曲名:Ravenous歌手:Arch Enemy专辑:Manifesto of Arch Enemy (Best Of)标题:Ravenous艺术家:Arch EnemyI am hunting for your soulIt dwells within your heartI lacerate the pounding fleshYour spirits shall be mineSo rise my spirit rise!Revel in this dead mans" bodyGrip his soul, sip the bloodLife is deathA holy carnageRavenousI will be your godCarnivorous JesusI need your fleshRitual slaughterFill up the chaliceWith the essence of your lifeLiquid strengthsTrapped in your veinsI crave your bloodYou must die!金属梦:66032164http://music.baidu.com/song/35235794
2023-07-11 01:44:281

Arch Enemy的《Nemesis》 歌词

歌曲名:Nemesis歌手:Arch Enemy专辑:Metal for the MassesNemesisArch EnemyDoomsday MachineNemesis2005-Doomsday Machine-Arch EnemyWe walk this earthWith fire in our handsEye for an eyeWe are nemesisWe are with youCountless vicious soulsFight! - Fighting for freedomUnited, we stand..we standWe are legionVoice of anarchyThis is revolutionCreating new disorderWe are enemyopponent of the systemcrushing hypocrisyslaying the philistineOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisA malicious fever burnsIn our heart, in our veinsYour blood, my bloodOur blood runs the same...the sameOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesisWe are NemesisOne for allAll for oneWe are strongWe are oneOne for allAll for oneWe are oneNemesishttp://music.baidu.com/song/60601557
2023-07-11 01:44:351

Arch Enemy的《The Zoo》 歌词

歌曲名:The Zoo歌手:Arch Enemy专辑:Khaos Legions (Japanese Edition)(Scorpions Cover)The Zoo -- Arch EnemyAlbum - 《Khaos Legions》 2011The job is done and I go outAnother boring dayI leave it all behind me nowSo many worlds awayI meet my girl, she"s dressed to killAnd all we gonna doIs walk around to catch the thrillOn streets we call the zoo!!!We eat the night, we drink the timeMake our dreams come trueAnd hungry eyes are passing byOn streets we call the zoo!!!We eat the night, we drink the timeMake our dreams come trueAnd hungry eyes are passing byOn streets we call the zoo!!!Enjoy the zooAnd walk down 42nd StreetYou Gonna be excited tooAnd you will feel the heatWe eat the night, we drink the timeMake our dreams come trueAnd hungry eyes are passing byOn streets we call the zoo!!!We eat the night, we drink the timeMake our dreams come trueAnd hungry eyes are passing byOn streets we call the zoo!!!YEAH~~~solo~THE ZOO!!http://music.baidu.com/song/18170966
2023-07-11 01:44:421

Arch Enemy “silent wars” 歌词

This is killing usFighting the truth a losing battleWe believe in nothingJust hatred for each otherTragic sinister serenadeTwisted melody shatters realityDying hopes for a better dayFragile dreams that break awayNo more, no moreThis pain must endWe have chosen to sufferBelieve in nothing but...Hatred is a vicious circleBetrayal is at handParalysed souls bleedingBegging for salvationDeeper and deeper the wounds we cutThe truth burning our fleshSilent war to set us freeStigmata of a tragedyNo more, no moreThis pain must endWe have chosen to sufferFeeling nothing but hateIt"s eating us up from the insideTaking our feelings away
2023-07-11 01:44:491

Arch Enemy的《Ravenous》 歌词

歌曲名:Ravenous歌手:Arch Enemy专辑:Wages Of Sin标题:Ravenous艺术家:Arch EnemyI am hunting for your soulIt dwells within your heartI lacerate the pounding fleshYour spirits shall be mineSo rise my spirit rise!Revel in this dead mans" bodyGrip his soul, sip the bloodLife is deathA holy carnageRavenousI will be your godCarnivorous JesusI need your fleshRitual slaughterFill up the chaliceWith the essence of your lifeLiquid strengthsTrapped in your veinsI crave your bloodYou must die!金属梦:66032164http://music.baidu.com/song/35231363
2023-07-11 01:44:561

Arch Enemy的《Ravenous》 歌词

* 回复内容中包含的链接未经审核,可能存在风险,暂不予完整展示! 歌曲名:Ravenous歌手:Arch Enemy专辑:Tyrannts Of The Rising Sun - Live In Japan标题:Ravenous艺术家:Arch EnemyI am hunting for your soulIt dwells within your heartI lacerate the pounding fleshYour spirits shall be mineSo rise my spirit rise!Revel in this dead mans" bodyGrip his soul, sip the bloodLife is deathA holy carnageRavenousI will be your godCarnivorous JesusI need your fleshRitual slaughterFill up the chaliceWith the essence of your lifeLiquid strengthsTrapped in your veinsI crave your bloodYou must die!金属梦:66032164http://music.b***.com/song/35235252
2023-07-11 01:45:081

arch enemy - end of the line 英文歌词带翻译 谢谢啦

end of the line welcome to the circusleave your brain outsidesit back - relaxas we feed you liesspoon fed stimulationglittering fashion fascinationyou are lost in the gamemedia corruptionyou"ll be amusedby the things we do to youbut in the bitter endthe clown is youend of the lineyou sold your soulfor a better lifeyou are better off deadyou had one lifeyou had one shotyou blew itand you knew itthe rot sets inno self controlat the end of the lineyou play the fool
2023-07-11 01:45:141

silverwing arch enemy歌词

"Silverwing"Fly with me on a starlit skyI"ll set you free... Yes, it can beA dream unchased is a life at wasteNever let them conquer your prideOur love is warm hereLike the image of a distant sunThis star will always shineAnd never, ever fade awayFly on... SilverwingFly with me... Stay free... SilverwingSeeking truths in spirit skiesWhich one of you will guide me tonight?The milky way tastes sour,Has heaven lost its power?So let us set sail on this sea of dreamsThe moon gets lost in the morning sunJust let the spinning wheel spinAnd let your life begin
2023-07-11 01:45:331

Arch Enemy的《Vultures》 歌词

歌曲名:Vultures歌手:Arch Enemy专辑:Tyrannts Of The Rising Sun - Live In JapanBreathing life into these mares where enemies turn into fleshThis is the abyss I fear the most – I feel their eyes staring at meWaiting with carnivorous patience, their memory timelessFor all they know I must succumb sometime…A solemn cry rises to the sky as heavy wings blacken outThe morning sun with infinite darkness. Am I dreaming or am I dead?Waiting with carnivorous patience, their memory timelessFor all they know I must succumb sometime…In search for my heart – Lost in this hellThe vultures are soaring above – Tearing me apartIn search for my heart – Lost in this hellThe vultures are soaring above – Tearing me apartScreaming Voices in my head. A sudden pain then deadly silenceDevouring the remains of my heart – I can feel it dyingWaiting with carnivorous patience, their memory timelessFor all they know I must succumb sometime…In search for my heart – Lost in this hellThe vultures are soaring above – Tearing me apartIn search for my heart – Lost in this hellThe vultures are soaring above – Tearing me apartPicking at the bones of my soulLeft me dead insideThe more I gave the more they tookLeft me dead insideIn search for my heart – Lost in this hellThe vultures are soaring above – Tearing me aparthttp://music.baidu.com/song/35235264
2023-07-11 01:45:401

Arch Enemy的《Secrets》 歌词

歌曲名:Secrets歌手:Arch Enemy专辑:Khaos Legions<- Intro melody - Michael ->Secrets -- Arch EnemyAlbum - 《Khaos Legions》 2011I"ve got a secretI just can"t tellI have created my own hellIf you knew what I knowIt would change your perfect worldLocked the vaultThrown away the keyMy lips are forever sealedBuried in a place you"ll never seeThis secret dies with meDon"t ask meFor I cannot tellWords can killSharp as a blade of steeeeel !Skeletons of the past !This burden, this painWere all these tears in vain ?Memories of the past returnThis one is going with me to the graveIt is beating me up insideTo that I must confessSecret weighing down my soulUntil I draw my very last breathDon"t ask meFor I cannot tellWords can killSharp as a blade of steeeeel !Skeletons of the past !Skeletons of the past !- solo -<- Lead - Michael ->Skeletons of the past !Skeletons of the past !<- Lead - Christopher -><- Outro melody - Michael ->http://music.baidu.com/song/35237340
2023-07-11 01:45:461

arch enemy

留下邮箱啊!!我有!!
2023-07-11 01:45:531

求翻译arch enemy乐队的avalanche歌词,谢谢!

Avalanche I stepped into an avalanche, it covered up my soul; when I am not this hunchback that you see, I sleep beneath the golden hill. You who wish to conquer pain, you must learn, learn to serve me well. 我走进了一场雪崩, 它掩盖了我的灵魂; 那时我不像你们看到的这样驼背, 我睡在金山之下。 你们这些想要克服痛苦的人, 得学会,学会把我伺候好。 You strike my side by accident as you go down for your gold. The cripple here that you clothe and feed is neither starved nor cold; he does not ask for your company, not at the centre, the centre of the world. 你们下来淘金时 偶然撞到了我。 你们给他衣裳和食物的这个瘸子 他既不饿也不冷; 他也没请求你们 来这世界中心陪伴他。 When I am on a pedestal, you did not raise me there; your laws do not compel me to kneel grotesque and bare. I myself am the pedestal for this ugly hump at which you stare. 在我登上神坛之时, 并不是你们把我推举上去的; 你们的律法也不能迫使我 卑颜屈膝俯首称臣。 我自己就是你们盯着看的 这丑陋驼包的基座。 You who wish to conquer pain, you must learn what makes me kind; the crumbs of love that you offer me, they"re the crumbs I"ve left behind. Your pain is no credential here, it"s just the shadow, shadow of my wound. 你们这些想要克服痛苦的人, 得明白什么能使我变得仁慈; 你们给我的爱的碎屑, 都已被我搁置一边。 你的痛苦可不是通行证, 它只是影子,我伤口的影子。 I have begun to long for you, I who have no greed; I have begun to ask for you, I who have no need. You say you"ve gone away from me, but I can feel you when you breathe. 我已经开始在渴求你, 在我已经没有贪欲的时候; 我已经开始想要见到你, 在我已经没有需要的时候。 你说你已经远离了我, 但在你呼吸时我能感觉到你。 Do not dress in those rags for me, I know you are not poor; don"t love me quite so fiercely now when you know that you are not sure, it is your turn, beloved, it is your flesh that I wear. 别为我穿着这些破衣烂衫, 我知道你并不贫穷; 别再这样猛烈地爱我 在你知道你并不确定的时候, 亲爱的,该由你来定夺了, 我销蚀的是你的肉体。
2023-07-11 01:46:001

哪能找到Arch enemy的专辑下载啊?不要电驴!

Black Earth (1996)http://rapidshare.com/files/90402544/_1996__Black_Earth.rar.htmStigmata (1998)http://rapidshare.com/files/93780815/AE_S_1998.rar.htmBurning Bridges (1999)http://rapidshare.com/files/93761894/AE_BB_1999.rar.htmBurning Japan Live (1999)http://rapidshare.com/files/90446036/_1999__Live_At_Liquidroom_Japan.rar.htmWages of Sin (2001)http://rapidshare.com/files/90466828/_2002__Wages_Of_Sin.rar.htmAnthems of Rebellion (2003)http://rapidshare.com/files/90486828/_2003__Anthems_Of_Rebellion.rar.htmDead Eyes See No Future EP (2004)http://rapidshare.com/files/90502522/_2004__Dead_Eyes_See_No_Future.rar.htmDoomsday Machine (2005)http://rapidshare.com/files/90529592/_2005__Doomsday_Machine.rar.htmRise of the Tyrant (2007)http://rapidshare.com/files/93780604/AE_ROTT_2007.rar.htmArch Enemy - Revolution Begins (EP)http://rapidshare.com/files/93780362/AE_RB_EP_2007.rar.htm请用IE手动下载,不要用迅雷等下载软件。
2023-07-11 01:46:151

arch enemy吉他手用什么效果器

你可以搜一下他们的设备图来看看
2023-07-11 01:46:221

求摇滚歌曲……

变形金刚2
2023-07-11 01:46:292

请给我推荐几个死亡摇滚的乐队

涅槃NIRVANA玛丽莲曼森
2023-07-11 01:46:482

跪求大敌乐队Arch Enemy - Bridge of destiny最后一段SOLO的谱子,最好是GTP的,邮箱334039026@qq.com

楼主请给我一份啊
2023-07-11 01:46:563

When The Music Stops 歌词

歌曲名:When The Music Stops歌手:Eminem专辑:The Eminem ShowMusic, reality, sometimes it"s hard to tell the differenceBut we as entertainers have a responsibility to these kidsPsyche!EminemIf I were to die murdered in cold blood tomorrowWould you feel sorrow or show loveOr would it matterCan never be the lead-off batter of thingsShit for me to feed offI"m see-saw battlinBut theres way too much at stake for me to be fakeThere"s too much on my plateAnd I came way too far in this game to turn and walk awayAnd not say what I got to sayWhat the fuck you take me for? a joke? you smokin crack?Before I do that, I beg Mariah to take me backI get up "for i get down, run myself in the ground, "for I put some wack shit outI"m tryin-a smack this one out the park, five-thousand markYa"ll steady tryin to drown the sharkAin"t gonna do nothin but piss me offLid to the can of whoop ass, just twist me offSee me leap out, pull the piece out, fuck shootin i"m just trying to knock his teeth outFuck with me now, bitch, let"s see you freestyleTalk is cheap, motherfucker if you"re really feeling froggish, leapYo slim, you gonna let him get away with that?He tried to play you, you can"t let him skate with thatMan I hate this crap, this ain"t rap,This is crazy the way we actWhen we confuse hip-hop with real life when the music stopsSwiftThere ain"t no getting rid of McVeighIf so you woulda triedThe only way I"m leavin this bitch is suicideI have died clinically, arrived back at my enemy"s crib with hennesy,Got drunk then I finished heI"m every nigga"s favorite arch-enemy.Physically fitted to be the most dangerous nigga with beefI spark willingly with a dillinger in the dark dilligentlyI"m not what you thinkI appear to be fucked upMentally endangeredI can"t stay away from a razorI just want my face in a paperI wish a nigger had a grenade to squeeze tight to awake neighbors for acresI murder youDanger had me turned into a mad man, son of sam, bitch, I"m surgicalI"l allergic to dyin, you think not? you got balls? We can see how largeWhen the music stopsKon ArtistI was happy having a deal at first,Thought money would make me happy butIt only made my pain worst,It hurts when u see ur friends turn their back on u dawgWhen u ain"t got nothing left but ur word and ur ballsN ur stress full of causeOf ur new friends they beggin with their hands outChecking for ur record when its sellingWhen it aint, that"s the end, no laughsNo friends no girlJust the gin u drink till u car spin u thenScreechDamn!CrashU slam into the wall and u fallOut the car, trying to crawl with one armAbout to lose it all in a pool of alcoholIf my funeral"s tomorrow, wonder if they would even call when the music stopsKunivaLet"s see how many of your men loyal,When i pull up looking for you,With a pistol sipping on a can of pennzoilI"m revved up, who said what would lead bust ur head would just explodeWith red stuff i"m hand cuffed tossed in the paddy wagonBraggin about how u shot it like a coward, bullets devour you showered youNiggars, if i was u niggas, i"ll run while given the chanceUnderstand i can enchance the spirit of manDeath itself, it can"t hurt me, just the thought of dying alone that reallyHurts me, u ain"t worthy to speak thoughts of cheap talkBe smart and stop trying to walk how g"s walk before we sparkHug the floor while we plan to the war with ur life, fuck the tour and the micI"ll rather fuck a whore with a knife, deliver that shit the coroner"s likeYou high hype poppin" shit in broad day light nigga ur a gonna at nightWhen The Music StopsProofInstigators, ?? pits in cagesLet loose and bit the neighbourswrist to razorsYa"ll don"t want war, you want talkIn the dark my dogs all bark like woofProof nigga I"m a wolf, get your whole roofCaved in like reindeer hoofsStomped the roof shake the floor tiles looseThe more ya"ll breach, the more I movesThis hell street, this is hardcore bluesPut a gun to rap checking all our jewels (nigga)Or make the news betcha all ya"ll moveWhen the uzi pop, you better drop when the music stopBizzareMusic"s changed my life in so many waysBrains confused and fucked since the 5th gradeLL told me to rock the bellsNWA said fuck the policeNow i"m in jail93 was strictly RNBFucked up hair cutListen to JodeciMichael Jackson, who go tell me i"m MikeAss cheeks painted whiteFucking Presilla at nightFlying down sunset smoking crackTransvestite in the frontEddi Murphy in the backMOP had me grindy and griddyMarilyn Manson, i dyed my hair blueAnd grew some tittiesLudacris told me to throw them bowlsNow i"m in the hospitalBroken nose and a fractured elbowVoices in my head, i"m going in shock,I"m reaching for the glock but the music stopsBanghttp://music.baidu.com/song/1325028
2023-07-11 01:47:201

当一个男人对你说想你(做什么都想你),还说i want you是什么意思?

结了婚的和没结婚的男人对你说同样的话,可意思就区别大了去了,你得先搞清楚那个男人是已婚还是未婚,然后再问吧
2023-07-11 01:46:083

何不食肉糜是什么意思?

何不食肉糜是晋惠帝执政时期的一个名词。意思是为什么不去吃肉糜粥呢?肉糜也就是指肉粥。后世也就用这个词来形容人们对于事物没有全面的认知,或者没有亲身经历就妄下评论或建议。这句话的出处是“何不食肉糜”这个典故出自晋惠帝司马衷,就是司马懿的重孙子。原文是这样的,大家可以参考一下帝尝在华林园,闻虾蟆声,谓左右曰:"此鸣者为官乎,私乎?"或对曰:"在官地为官,在私地为私。"及天下荒乱,百姓饿死,帝曰:"何不食肉糜?"其蒙蔽皆此类也。这个故事是讽刺晋惠帝不知民间疾苦,当百姓没有食物吃的时候,他反问为什么不喝肉粥呢?这个成语就流传了下来。这个典故主要想告诉我们的,就是故事说的是有一年闹了饥荒,没有粮食吃的百姓扒树皮,吃草根,更有甚者为了填饱肚子去吃观音土而被活活胀死。消息一次层层上报到了皇帝这里,皇帝是个善良的人,在得知这种消息后很是为他的百姓们担忧。苦思冥想后对着身边的人问道:“百姓无粟米充饥,何不食肉糜?”(百姓没有米粮可以充饥,为什么不去吃肉糜粥呢?)单独读这个故事我们可能会以为这个皇帝的智商怕不是有问题,米都没有,何谈吃肉。但是对于历史人物,尤其是能坐上皇帝位子的人,我们决不能通过只言片语就给这个人下定论。要从历史发展的角度去判断这个问题。司马家夺了曹魏的江山后,虽然很快统一了天下,但其实内部的矛盾仍然十分激烈。天下熙熙皆为利来,庙堂蝇蝇皆为名往,对于这个本就是抢来的皇帝位子,心动的人可不是一个两个。司马衷的父亲是大名鼎鼎的的司马炎,就是西晋的开国皇帝。本来皇位轮不到司马衷这个次子来坐,可是他的兄长司马轨不幸早夭,加上司马炎虽然对儿子不大满意,但对于孙子司马遹却甚为喜爱。况且当时还有司马昭的次子司马攸(过继给司马师延续香火)对皇位虎视眈眈,于是司马衷便成为了太子。每当我们嘲笑司马衷的“何不食肉糜”时,我们又何尝不是在“何不食肉糜”一样片面的看待一个君王。
2023-07-11 01:46:081

如何理解致病性分化?

differenciation of pathogeni-city李振岐一种病原物的不同菌株对寄主植物中不同属、种或品种的致病能力的差异,也称寄生专化性(parasitic specialization)或生理专化性(physiologic specialization),一般说来,寄生性程度越高的病原物其致病性分化程度越高如麦类锈菌、白粉菌等。寄生性程度越低的病原物其致病性分化程度也越低如一些兼性寄生菌。人类发现植物病原物的致病性分化现象已有100多年历史。1894年瑞典埃里克森(J.Eriksson)通过对秆锈菌试验证实了病原物的致病性有分化现象,并根据在不同属、种、品种上的反应差异,证实禾谷类秆锈菌有六个专化型。1917年美国斯坦克曼(Elvin Charles Stakman)发现,在小麦秆锈病菌的专化型内还有小种的分化。1950年在研究小麦品种Thatcher丧失抗秆锈性过程中又发现在小种内还有生物型的分化。这些研究结果在理论上和技术上为以后开展病原物致病性的分化研究奠定了基础。分化现象的形成病原物的致病性分化现象是在病原物与其寄主植物长期演化过程中相互适应和选择下形成的。与寄主植物的抗病性类型相对应,一般可分为专化(或垂直)致病性和非专化(或非垂直)致病性。专化致病性与专化抗病性和非专化致病性与非专化抗病性是两组互为前提而共现的生物学性状。弗洛尔的“基因对基因”学说指出:在进化过程中寄主群体中有一个控制抗病性的基因,病原物群体中就相应地有一个控制致病性的基因。病原物与寄主共同发展过程如下:腐生微生物克服了高等植物的自然免疫性,获得了侵袭力,由腐生演化到寄生,开始具有一般致病性,而寄主方面也具有一般抗病性。在继续长期共存中,寄主和病原物由于多种变异和相互选择,寄主方面产生了专化抗性,而病原物方面或迟或早产生能克服这种专化抗性的毒性基因。(见基因对基因概念)病原物专化致病性的特点,是与其所适应的寄主不同品种之间有特异性或专化性互作关系,而病原物的非专化致病性的特点,是与其所适应的寄主的不同品种之间无特异性互作关系。毒力病原物的一定菌系对具有一定抗病基因品种的专化性和垂直致病力。又称毒性。研究病原物的毒性主要采取分析病原物小种、毒力频率和联合致病性的方法。小种病原物种、变种或专化型以下的分类单位。小种之间在形态上无差异,区别不同的小种(race),主要根据它们对具有不同抗病基因的鉴别品种的致病力差异。细菌的小种有时称为菌系(strain)或致病型(pathotype)。小种鉴定方法不同病原物小种的鉴定方法不完全相同。病原真菌和细菌小种的鉴定大多是采用一套鉴别品种,根据供测菌株在鉴别品种上的致病力表现来确定小种,目前鉴定病菌的小种有的(如鉴定小麦秆锈病的小种)采用国际通用鉴别寄主,有的(如鉴定小麦条锈病菌的小种)采用变动鉴别寄主,有的(如鉴定马铃薯晚疫病菌、稻瘟病菌和稻白叶病菌等的小种)采用已知基因或单基因品种作为鉴别品种。此外,鉴别非专化寄生物的小种还可根据病原物的生理生化性状,培养性状,血清学和荧光反应等作辅助鉴别。病毒株系间的区别主要根据它们在一定寄主上的症状差异。区别是否为同种病毒的不同株系,也可根据血清学反应和彼此是否有交互保护作用以及是否有相似的寄主范围和传播方式来鉴定。小种命名方法不同病菌小种的命名方法也不完全相同,有的采用顺序编号法即按国际统一编号如小麦秆锈病菌;或按国家或地区编号,如小麦条锈病菌命名;有的如燕麦秆锈菌采用毒力公式法命名,即用对该小种有效的抗病基因作分子,无效的抗病基因作分母写成的公式:无毒力(R)/有毒力(S);有的如稻瘟病菌采用分段加数(加抗或加感)法命名。小种鉴定程序一般有五个步骤,如小麦锈菌小种的鉴定程序如下:①菌种采集。采集菌种标样是做好小种鉴定的首要环节。一般采自不同地区、不同品种田间病株或病叶,要从新发病的品种上采集。标样采得后应分别装入玻璃纸袋内,防止混杂和污染,并注明采集地点和时间,以备登记和分析。②菌种纯化和繁殖。纯化菌种是取一个新鲜夏孢子堆,作单菌株隔离繁殖,在菌种少时,也可先将标样接种到有代表性的鉴定品种幼苗上,待发病后再挑取不同反应型的单个孢子堆,隔离繁殖。菌种繁殖在温室内高感品种上进行。③菌种保存。保存的方法很多,最常见的低温保存法和冷冻干燥保存法。低温保存法是将培养的菌种保存在4~8℃的冰箱中,每隔6~8个月移植一次,注意防止污染。一些生活力较差的病原真菌可保存在-20℃的低温冰箱中。近年来应用超低温(用液态氮保持-196℃或用干冰保持-70℃)保存菌种的越来越多,其优点是保存时间长,保存效果好,不足之处是需要较多的设备。冷冻干燥保存方法也是当前常用的保存菌种的方法之一。④接种鉴别寄主。鉴别品种一般播种在花盆内,每盆2~4个品种,相互隔开,中间插播高感对照品种。接种方法,可根据情况,选用手指涂抹法、喷雾法等。接种后,隔离,保温,然后在适温下培养。⑤鉴定小种类型。接种后,经一定时间,当被测品种的反应型稳定后,尤其是感病品种发病稳定后,进行记载。记载项目主要是反应型,其次是病株率和严重度,然后将记载的反应型与已知小种在鉴定品种上的反应进行比较,以确定小种的种类。同一小种的致病力也可进一步分化出不同的致病类型,称为生物型(biotype),即小种内由遗传上一致的个体所组成的群体。在一个小种内可有一个生物型,也可有多个生物型。鉴定小种的生物型主要根据供试菌系在辅助鉴别品种上的反应。毒力频率和联合(综合)致病性毒力频率(virulence frequency)是一种病原物群体中对一定抗病品种(抗病基因)有毒力的菌株(毒性菌株)出现频率。毒力频率(%)=有毒力菌株数/总菌株数×100。联合致病性(pathogenicity association)是一种病原物的群体中对二个以上被测品种(抗病基因)有毒力的菌株(毒性基因)出现频率(%)。毒力频率分析反映一个被测品种与一个病原物群体中多个小种(菌株)的相互关系,而联合致病性分析则反映2个以上被测品种与一个病原物群体中多个小种的相互作用。有了这两方面的分析结果,育种和品种利用工作的依据就会更为充分。寄主适合度指寄生物在寄主上的定殖能力、繁殖或产孢速度及数量等适应能力。寄生适合度高,两者为亲合组合,其中病原物的侵染能力(侵染力)愈强。致病性相关基因pathogenicity related genes何晨阳病原物中决定对植物致病性的有关基因。致病基因决定了病原物在侵染植物过程中,与植物建立寄生关系和破坏植物正常生理功能,调控着对植物的吸附、侵入并在植物中定殖、扩展,最终破坏寄主同时显示症状的过程。类型根据功能分为毒性基因、无毒基因和决定寄主范围的基因。毒性基因决定对植物基本亲和性的基因,调控病害发生所必需的致病过程。根据基因产物性质,已知的毒性基因(virulence genes)包括胞外降解酶基因、毒素基因、激素基因、胞外多糖基因以及未知产物基因。胞外降解酶基因包括结构编码基因,调节基因和分泌基因。降解酶包括果胶酶、纤维素酶、蛋白酶、半纤维素酶和植保素降解酶等。对于这些性质清楚的致病因子的基因克隆,可以在平板上直接检测克隆DNA产物。未知产物的毒性基因已发现hrp基因和dsp基因两类。hrp基因决定病原物对寄主植物的致病性和诱导非寄主植物过敏性反应。dsp基因决定病菌侵袭力并参与代谢的能力。对于未知产物毒性基因的克隆,通常用物理、化学或生物诱变法,诱导病原物中与致病性相关基因突变,获得相应的突变体。用基因库互补法从基因文库中筛选出能够互补突变体功能的重组克隆;或者用分子杂交法,与突变基因序列杂交,从基因文库中获得目的基因克隆。无毒基因决定对寄主植物特异性不亲和性的基因,亦称为寄主专化性基因或反向调节的寄主范围基因。在病原物与寄主植物之间存在基因对基因的关系中,病原物无毒基因(avirulence genes)表达,与寄主植物中相对应抗病基因互作,从而导致不亲和反应。病原物在植物中的定殖和扩展受抑制,或者在侵染初期就破坏了亲和关系。病原物无毒基因不仅决定了对植物不同品种的无毒性,也决定了对植物不同种和非寄主植物的无毒性。从病原细菌、真菌和病毒中都已克隆到无毒基因。如从丁香假单胞菌大豆致病变种6号小种克隆的avr A,黄枝孢(番茄叶霉病菌)中的avr9和烟草花叶病毒(TMV)的具有无毒基因功能的外壳蛋白基因。然而对无毒基因的产物特征和功能尚未完全清楚。一般认为无毒基因直接或间接地编码了激发子的产生。如番茄叶霉病菌avr9编码了63个氨基酸的多肽,这个专化性激发子激发了番茄中带有相应抗病基因cf9的品种的过敏性反应。丁香假单胞菌番茄致病变种中的avrD的产物是酶,能将细菌代谢物转化为低分子量的脂类激发子而释放出去。克隆无毒基因常用基因文库互补法。将病原物基因文库DNA向其它小种、致病变种或其它不同种病菌中转移,通过测定转化接合子对植物的致病表型,筛选出能使受体菌对特定植物表现为无毒性的重组克隆,获得无毒基因。寄主范围决定基因扩大病原物寄主范围的基因。从青枯病假单胞菌花生菌株基因文库中重组克隆DNA,导入对花生不致病的番茄菌株,使得后者变得对花生致病。从根癌土壤杆菌广寄主范围的菌株基因文库中获得的重组DNA克隆,能使寄主范围较窄的葡萄菌株扩大其侵染植物的种类。性状病原物致病基因数量众多,大多数成簇排列,并高度保守,具有多效性功能。数量致病基因是病原物对植物致病过程所必需,而体外生长并不需要的基因。根据突变体致病基因突变频率和营养突变频率推算,病原细菌致病基因数量有50~100个。从细菌已鉴定和分离出50多个致病基因,但这些基因并不一定共存在某一个病原菌中。成簇性致病基因定位于染色体上和(或)质粒上。大多数致病基因都是成簇排列的。根癌土壤杆菌致病基因主要集中排列在质粒上的T区和V区,V区就包含了virA、virB、virC、virD、virE、virF、virG、virH8个调节子。病原细菌中的hrp基因也是大基因簇。丁香假单胞菌菜豆致病变种hrp基因簇中含有9个互补群,全长达22千碱基对。青枯病假单胞菌(Pseudomonas solanacearum)hrp基因DNA片段长达17~22千碱基对,至少有9个转录单位。胞外降解酶及泌出基因、多糖合成酶基因等都是以基因簇方式存在。菊欧文氏菌果胶酶的5种同工酶基因以两个基因簇方式存在。油菜黄单胞菌油菜致病变种(甘蓝黑腐病菌)与胞外酶泌出和多糖合成有关的基因也是成簇的。保守性由于营养要求和生化代谢的基本相似性,从一种病原物中克隆的致病基因,可以在该病原物的其它小种、致病变种、其它种病原物和非病原物甚至动物病原物中发现其结构上同源序列,其产物的生化特征也基本类似,但并不一定具有相同的致病功能。hrp基因在丁香假单胞菌许多致病变种中是同源的;青枯病假单胞菌的hrp基因和油菜黄单胞菌不同致病变种之间也具有同源性(见图)。hrp基因在某些病原细菌中可以相互交换,因此向其它细菌导入hrp基因可以导致寄主防卫反应。从丁香假单胞菌丁香致病变种克隆的32kb的hrp大基因簇片段,在丁香假单胞菌烟草致病变种、荧光假单胞菌(Pseudomonas flurescens)或大肠杆菌(E coli)中表达,可以导致烟草植株的过敏性反应。根癌土壤杆菌和油橄榄假单胞菌中与生长素和细胞分裂素生物合成有关的基因也同源。尽管无毒基因具有不同的专化性功能,但许多无毒基因之间存在明显的序列同源性。如从油菜黄单胞菌辣椒致病变种中克隆的avr Bs3与其它致病变种中的无毒基因高度同源。油菜黄单胞菌水稻致病变种(水稻白叶枯病菌)avr10也发现有广泛同源性,不仅在不同小种中有同源序列,在其它致病变种和其它属中也有同源性。青枯病假单胞菌和油菜黄单胞菌油菜致病变种hrp基因区的结构同源性(黑点区和斜线区表示相互之间的同源区)(引自Arlat,M et al1991)多效性致病基因突变对病原物表型改变具有多效性。无毒基因突变,可以使病原物对特定寄主表现毒性,避免了植物过敏性反应的激发和识别过程发生。突变的无毒基因使寄主相应的抗病基因丧失功能,但并不明显地影响病原物的毒性或其他生物学性状。毒性基因突变,在致病性和寄生性上的影响不同。有些突变体在同源寄主上表现不完全的致病性,或者全部丧失,或者部分降低,对非寄主植物诱导过敏性反应的能力也有影响。有些突变体可以在植物体内生长和定殖,但不产生任何症状。致病基因的突变还可以赋予病原物丰富多样的生化表型,与各种胞外降解酶、多糖、毒素和激素等致病生化因子发生连锁改变,有的致病生化因子产生能力低于野生型菌株,有的却比野生型菌株高。表明了致病基因的复杂性以及致病基因与代谢途径中涉及的有关基因之间存在着可能的调控关系。表达调控许多病原物致病基因的表达受到植物组分的诱导,并受到双组分调控系统的调控。植物组分的诱导目前已有三种方法用植物组分对致病基因诱导作用的研究。①诱导性启动子探针途径是用启动子探针鉴别出受寄主植物诱导的病原物的启动子,筛选基因文库中与该启动子同源序列,从而分离出完整的受启动控制的致病基因。②用植物诱导病原物产生的多肽制备抗血清。筛选表达性基因文库,分离结构基因;或者用诱导的和非诱导的mRNA转录成cDNA进行特异性杂交,鉴别表达受调控的基因。③基因融合法。是在致病基因序列后插入转座子的一部分形成报导基因(如lux,cat lacZ),产生基因融合体,指示致病基因的表达及其调控。植物细胞组分起着诱导病原物致病基因表达的刺激信号的功能。这些组分有酚类、糖类以及性质尚不清楚的低分子量物质。如许多双子叶植物受伤后释放出一些酚类物质,尤其是乙酰丁香酮和羟基乙酰丁香酮,刺激了根癌土壤杆菌V区基因的活化和表达。许多病原细菌中hrp基因,丁香假单胞菌丁香致病变种中的sym B基因,玉米萎蔫欧文氏菌中的wts基因等表达与寄主植物的诱导有关。一些致病基因在植物体内,在无机培养基上表达水平高,而在复合培养基上不表达或表达量低。双组分调控机制植物病原细菌致病基因表达调控的一种主要途径。典型双组分调控系统由一对感受蛋白和调节蛋白组成,分别由两个不同基因编码。感受蛋白一般为跨膜蛋白,能感受胞外环境的刺激信号,经变构传入胞质。感受蛋白N端感受的信号,经过保守的C端与调节蛋白的保守的N端互作,通过磷酸化过程进行信号传递、被磷酸化的调节蛋白具有在转录水平上调控其它基因表达的功能。在许多双组分系统中,调节蛋白是DNA结合蛋白,能特异性地与基因启动子上游的DNA序列结合,激活基因的转录。所有双组分调控系统的感受蛋白或调节蛋白在氨基酸序列上高度保守。如感受蛋白C端约250个氨基酸具明显的同源性,N端虽不具同源性,但大多有多个疏水区。根癌土壤杆菌毒性基因virA和virG所编码的VirA和VirG组成了双组分调控系统。virA为跨膜蛋白,直接感受植物从伤口释放出的酚类和糖类物质,然后将信号传递给调节蛋白virG,后者活化后调控其它的vir基因的表达,而vir基因激活后,促进了转移DNA(T-DNA)向寄主植物细胞转移和整合。许多细菌hrp基因表达也受到双组分调控系统调控。
2023-07-11 01:46:111

微电子学与机械电子工程有什么区别,考研哪个比较有前途,—我本科是机械电子专业的

就目前的情况来看,其实选择学电脑是很不错的,出来基本不愁找工作的问题,还有些也可以自己创业都行。
2023-07-11 01:46:084

这深海无人啊是什么歌

《享用悲伤》。根据查询QQ音乐显示:《享用悲伤》歌手为FlyN,专辑为享用悲伤,相关歌词:就让我沉入这个深海无人打扰,就让这无尽的黑暗成为我的依靠。FlyN来自澳门的水瓶男孩,相关作品:《习惯》、《享用悲伤》、《没有爱情的爱》等。
2023-07-11 01:46:061

机械加工中的pocket是什么?

pocket可以指被加工物体表面一块近似凸多边形的区域,可以通过从外轮廓不断向内偏移得到一组可行的加工路径,也可以指刀槽、减重槽之类的凹槽形特征。
2023-07-11 01:46:042