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Boy George的《Funtime》 歌词

2023-07-23 21:57:37
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歌曲名:Funtime
歌手:Boy George
专辑:Cheapness And Beauty

Funtime
Pop/bowie
Fun
Hey baby we like your lips
Fun
Hey baby we like your pants
All aboard for funtime
Fun
Hey, I feel lucky tonight
Fun
I"m gonna get stoned and run around
All aboard for funtime
Fun
Last night I was down in the lab
Fun
Talkin" to dracula and his crew
All aboard for funtime
Fun
I don"t need no heavy trips
Fun
I just do what I want to do
All aboard for funtime
Fun
Baby baby we like your lips
Fun
Baby baby we like your pants
All aboard for funtime
Fun
Everybody we want in
Fun
We want some we want some
All aboard for funtime
Fun
Baby baby we like your lips
Fun
Baby baby we like your pants
All aboard for funtime
q421654485

http://music.b***.com/song/3464743

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2023-07-23 18:55:481

Birthday Boy 歌词

歌曲名:Birthday Boy歌手:Pet Shop Boys专辑:Release-EMI Repeatbirthday boypet shop boysreleasebirthday boy can"t explainchristmas eve he"s born againon the line a broken train going nowhere endless rainbirthday boy so afraid plays the machines in the arcadeoh baby do you remember he"s been through all this beforespent the summer getting nowheretaking all of the blame for the citycalculating from pain comes pitychristmas eve a time of joy if you believe in birthday boythis time around it"s all a mistake is he deluded or just a fake?oh baby do you remember he"s been through all this beforespent the summer getting nowheretaking all of the blame for the citycalculating from pain comes pityif you knew his name whould you feel the same?if you knew his name?birthday boy stands apart as the drama"s about to start a quick betrayal a speedy trial as before complete denialoh baby do you remember he"s been through all this beforespent the summer getting nowheretaking all of the blame for the citycalculatingfrom pain comes pity if you knew is name would you feel the same?http://music.baidu.com/song/628780
2023-07-23 18:56:071

to himself about boy the time behave is it learnt

It is about time the boy learnt to behave himself.现在是孩子学会循规蹈矩的时候了。
2023-07-23 18:56:151

有人知道有关什么什么的boy的英文歌吗?

lie awake at night晚上我躺在床上没有一点睡意 See things in black and white世界对我来说只有黑与白 I"ve only got you inside my mind你的形象无时无刻不在我脑中闪动 You know you have made me blind我的眼里只有你 I lie awake and pray我清醒地躺在床上祈祷 that you will look my way祈祷你会看见我 I have all this longing in my heart我的心中充满渴望 I knew it right from the star一开始我就知道t Oh my pretty pretty boy I love you我的漂亮男孩 我爱你 Like I never ever loved no one before you在你之前我从未这样爱过一个人 Pretty pretty boy of mine我的漂亮男孩 Just tell me you love me too告诉我你也爱我 Oh my pretty pretty boy I need you我的漂亮男孩我需要你 Oh my pretty pretty boy I do我的漂亮男孩,是真的 Let me inside make me stay right beside you让我进来让我留在你身旁 I used to write your name我曾写下你的名字 And put it in a frame并把它框起来 And sometime I think I hear you call Right from my bedroom wall有时我觉得我在我房间里听到了你的呼唤 You stay a little while你待了一会儿 And touch me with your smile 用你的微笑打动我的心 And what can I say to make you mine我该说什么才能赢得你的心 To reach out for you in time才能及时掳获你 Oh my pretty pretty boy I love you Like I never ever loved no one before you Pretty pretty boy of mine Just tell me you love me too Oh my pretty pretty boy I need you Oh my pretty pretty boy I do Let me inside make me stay right beside you 我的漂亮男孩 我爱你 在你之前我从未这样爱过一个人 我的漂亮男孩 告诉我你也爱我 我的漂亮男孩 我需要你 我的漂亮男孩,是真的 让我进来 让我留在你身旁 Oh pretty boy pretty boy pretty boy Say you love me too Oh my pretty pretty boy I love you Like I never ever loved no one before you Pretty pretty boy of mine Just tell me you love me too Oh my pretty pretty boy I need you Oh my pretty pretty boy I do Let me inside make me stay right beside you 我的漂亮男孩 我爱你 在你之前我从未这样爱过一个人 我的漂亮男孩 告诉我你也爱我 我的漂亮男孩 我需要你 我的漂亮男孩,是真的 让我进来 让我留在你身旁
2023-07-23 18:56:231

The boy spends most of his time ____(study).

两个都对spendsometime(in)doingsth=spendsometimeonsth花费时间去做某事那个in可以省略
2023-07-23 18:56:301

4个sometime的顺口溜

区别顺口溜:分开是“一段”(some time),相聚为“某时”(sometime);分开s是“倍、次”(some times),相连s是“有时”(sometimes)。具体区分:1、sometime表示“某个时候”,可以是过去也可以是将来。I will tell you this secret sometime in the future.我以后会告诉你这个秘密。2、sometimes表示“有时候”,相当于occasionally,常用于一般现在时的时间状语。The naughty boy sometimes fights with others.这个淘气的男孩有时和别人打架。3、some time表示“一段时间”。I need some time to finish the rest work.我需要一些时间来完成剩下的工作。4、some times表示“几次,几倍”。I have read this novel some times.我读过这本小说好几次了。例句:(1)I"ll stay here for some time.我将在这儿呆一段时间。(2)Kate will be back sometime in February.凯特将在2月某个时候回来。(3)Our school is some times larger than theirs.我们学校比他们学校大几倍。(4)Joan goes to school on foot,but sometimes by bike.琼步行上学,但有时也骑自行车去。
2023-07-23 18:56:431

now boy是什么意思?

现在男孩。重点词汇boy男孩;男青年;年少的儿子;做某工作的男孩;伙计;表示惊奇、高兴。A victory for me I think, it is only a matter of time now boy.看起来我会胜利,这只是个时间问题,小子。Now the boy is quite a help to his mother.现在这男孩已很能给他妈妈帮点忙了。女用人;Now the boy had everything he wanted.现在,这个男孩拥有他需要的一切了。" Now, boy, tell us what"s the matter: don"t be afraid.行了,孩子,告诉我们是怎么回事,别害怕。
2023-07-23 18:57:031

Jojo的《This Time》 歌词

歌曲名:This Time歌手:Jojo专辑:The High RoadThis timeThis timeI made the mistake like beforeGivin you the third degree, takin you through itI told you I get what I wantAnd I ain"t tryna hear nothin else but just do itAnd I had to take a good lookWhen everything else didn"t have a ring to itI"m sittin here blamin myselfWhen I saw what you were feelin inside, I misused itThis time it"s all my fault (Ooh oh)This time I can"t let goThis time I did itReally, but I got these feelingsHow am I supposed to know? (Ooh oh)Can"t let my weakness show (Yeah yeah)Havin my fits may cause me to miss this first chance at real loveThis time I won"t let you get away from meThis time I"m gonna let you take it where it should beThis time I won"t say noCause sayin no is a way to protect meBaby this time (this time) this time (this time) this time (this time)I"mma let you want meEvery female I knowCan"t seem to take her eyes off of you and I see itIt"s just that my girls been so hurtAnd I"m not tryna be the next one goin through itI see that I got somethin realAnd I don"t wanna lose it and gotta to say thank youThe thought of you gone gives me chillsSo baby let"s just put this aside and just come hereThis time it"s all my fault (Really all my fault)This time I can"t let goThis time I did itReally, but I got these feelings (And I got these feelings)How am I supposed to know? (To know)Can"t let my weakness showHavin my fits may cause me to miss this first chance at real loveThis time I won"t let you get away from meThis time I"m gonna let you take it where it should beThis time I won"t say noCause sayin no is a way to protect meBaby this time (this time) this time (this time) this time (this time)I"mma let you want meNever will I deny you love meAll this time you stuck here and didn"t leave (my time)Now I know what "trust me you will see" was (And I owe you)All my heart and deepest apologiesSo let"s take this back to we got it manI just wanna do this with you boyThis time I won"t let you get away from meThis time I"m gonna let you take it where it should beThis time I won"t say noCause sayin no is a way to protect meBaby this time (this time)this time (this time)this time (this time)I"mma let you want meThis time I won"t let you get away from meThis time I"m gonna let you take it where it should beThis time I won"t say noCause sayin no is a way to protect meBaby this time (this time)this time (this time)this time (this time)I"mma let you want meBy Cassiehttp://music.baidu.com/song/1562278
2023-07-23 18:57:341

找一首英文歌 高潮是 boy boy B L boy

是boy boy be my boy 吧?歌名是:Boys smile唱的boys boys be my boy I wanna feel your body boys boys be my boy tonight I"m ready honey boys boys be my boy I wanna be your lover girl be my lover boy boys boys be my boy I wanna feel your body boys boys be my boy tonight I"m ready honey boys boys be my boy I wanna be your lover girl be my lover boy hot summer time always something on my mind a kind of magic in the air I"m on the run to a sunny paradise with crowed beaches everywhere I"m just a girl who"s looking for some fun it"s time to get things started so common everybody boys boys be my boy I wanna feel your body boys boys be my boy tonight I"m ready honey boys boys be my boy I wanna be your lover girl be my lover boy hot summer night always makes me loose control it feels like heaven now I"m free and all the boys if you want to feel the heat just come along and play with me I"m just a girl who"s looking for some fun it"s time to get things started so common everybody repeat** boys boys be my boy I wanna feel your body boys boys be my boy tonight I"m ready honey boys boys be my boy I wanna be your lover girl be my lover boy Can"t live with em" Cant live without em"!
2023-07-23 18:57:431

平家boy什么意思

男孩
2023-07-23 18:57:524

prtty boy歌的中文歌词

Ilieawakeatnight晚上我躺在床上没有一点睡意Seethingsinblackandwhite世界对我来说只有黑与白I"veonlygotyouinsidemymind你的形象无时无刻不在我脑中闪动Youknowyouhavemademeblind我的眼里只有你Ilieawakeandpray我清醒地躺在床上祈祷thatyouwilllookmyway祈祷你会看见我Ihaveallthislonginginmyheart我的心中充满渴望Iknewitrightfromthestar一开始我就知道tOhmyprettyprettyboyIloveyou我的漂亮男孩我爱你LikeInevereverlovednoonebeforeyou在你之前我从未这样爱过一个人Prettyprettyboyofmine我的漂亮男孩Justtellmeyoulovemetoo告诉我你也爱我OhmyprettyprettyboyIneedyou我的漂亮男孩我需要你OhmyprettyprettyboyIdo我的漂亮男孩,是真的Letmeinsidemakemestayrightbesideyou让我进来让我留在你身旁Iusedtowriteyourname我曾写下你的名字Andputitinaframe并把它框起来AndsometimeIthinkIhearyoucallRightfrommybedroomwall有时我觉得我在我房间里听到了你的呼唤Youstayalittlewhile你待了一会儿Andtouchmewithyoursmile用你的微笑打动我的心AndwhatcanIsaytomakeyoumine我该说什么才能赢得你的心Toreachoutforyouintime才能及时掳获你OhmyprettyprettyboyIloveyouLikeInevereverlovednoonebeforeyouPrettyprettyboyofmineJusttellmeyoulovemetooOhmyprettyprettyboyIneedyouOhmyprettyprettyboyIdoLetmeinsidemakemestayrightbesideyou我的漂亮男孩我爱你在你之前我从未这样爱过一个人我的漂亮男孩告诉我你也爱我我的漂亮男孩我需要你我的漂亮男孩,是真的让我进来让我留在你身旁OhprettyboyprettyboyprettyboySayyoulovemetooOhmyprettyprettyboyIloveyouLikeInevereverlovednoonebeforeyouPrettyprettyboyofmineJusttellmeyoulovemetooOhmyprettyprettyboyIneedyouOhmyprettyprettyboyIdoLetmeinsidemakemestayrightbesideyou我的漂亮男孩我爱你在你之前我从未这样爱过一个人我的漂亮男孩告诉我你也爱我我的漂亮男孩我需要你我的漂亮男孩,是真的让我进来让我留在你身旁
2023-07-23 18:58:471

The More Boys I Meet 歌词

歌名:The More Boys I Meet演唱:Carrie Underwood作词:Kennedy, Gordon Scott/Mc Ewan, Steve作曲:Kennedy, McEwan所属专辑:Carnival Ride发行时间:2007-10-23发行公司:Arista英文歌词:This boy here wants to move too fastHe see"s my future as having a pastWell, I don"t think soI don"t think soThat boy there, well he"s playing a foolHe thinks he"s funny and he thinks he"s coolWe"ll I don"t think soI don"t think soCheap date, bad taste of nothing like gone to wasteTalking about nothing in so many wordsIt"s not like I"m not trying"Cause I"ll give anyone a shot onceAnd, I , I close my eyesAnd, I kiss that frogEach time findingThe more boys I meet the more I love my dogHere"s this guy, thinks he"s bad to the boneHe wants to pick me up and take me homeWell, I don"t think soI don"t think soCage fights, PlayStation, X-Games rated nationOversize paints with an ego to matchIt"s not like I"m not trying"Cause I"ll give anyone a shot onceAnd, I , I close my eyesAnd, I kiss that frogEach time findingThe more boys I meet the more I love my dogWhy can"t they be like the one"sthat mean everything to meThe one and loyal, open and friendlyIt"s not like I"m not trying"Cause I"ll give anyone a shot onceI close my eyesAnd, I kiss that frogEach time findingThe more boys I meet the more I love myI close my eyesAnd, I kiss that frogEach time findingThe more boys I meet the more I love my dogThe more I love my dog
2023-07-23 18:58:552

the new boy 歌词翻译

travis - new amsterdamjean-michel basquiat, francois truffaut,robert zimmerman and de niroparis, texas - end of the worldnew york, new york,good bye girland they meeton blaker streetor the park that is centraloh noi watched the sun go downdown down beneath the groundand it"s a new day,it"s a new dawn,in new amsterdam.the stranger in the moonlight,looks stranger in the moonlightand they meeton blaker streetor the park that is centraloh noi watched the sun go downdown down beneath the groundand it"s a new day,it"s a new dawn,in new amsterdam.and we meeton blaker streetor the park that is centraloh noi watched the sun go downdown down beneath the groundand it"s a new day,it"s a new dawn,in new amsterdam.wait for the hidden track...sailing awaywhat are we gonna do,when you"ve stopped crying?what ever you"re going throughit"s not worth dying.chasing away the blues,i know you"re trying.and nobody wants to lose,i don"t know why.cause i live by the river,live by the river andi"ll die by the riveri"m sailing, away, today.what are you gonna saywhen they stop laughing?you"re giving it all awaywhen you"ve got nothing.how many times a dayyou feel like walking?taking a holiday fromall the shy-y-y...but i live by the river,live by the river andi"ll die by the riveri"m sailing, away, today.cause i live by the river,live by the river andi"ll die by the riveri"m sailing, away, today.and you"ve got to grabthe bull by the horns my friendit"s the only way to go.and when the story"s toldwe can go home.so what are we gonna do,when you"ve stopped crying?what ever you"re going throughit"s not worth dying.keeping away the bluesyou know i"m trying.what"ve we got to lose,and testify-y-y...cause i live by the river,live by the river andi"ll die by the riveri"m sailing, away,i"m sailing, away,i"m sailing, away, today.end
2023-07-23 18:59:034

Country Boy 歌词

歌曲名:Country Boy歌手:Alan Jackson专辑:Now That"s What I Call Country Volume 2Alan Jackson - Country BoyAlbum: Good TimeExcuse me ma"am, I saw you walk inI turned around, I"m not a stalkerWhere you goin? Maybe I can help yaMy tank is full, and I"d be obliged to take yaCause I"m a country boy, I"ve got a 4 wheel drivePile in my bed, I can take ya for a rideUp city streets, down country roadsI can get ya where you need to goCause I"m a country boyYou sure look good, sittin" in my right seatBuckle up, I"ll take you through the five speedsWind it up, or I can slow it way downIn the woods or right uptownI"m a country boy, I"ve got a 4 wheel drivePile in my bed, I can take ya for a rideUp city streets, down country roadsI can get ya where you need to goCause I"m a country boyBig 35"s whinin" on the asphaltGrabbin" mud, and slingin" up some red dirtCause I"m a country boyMy muffler"s loud, dual Thrush tubesI crank the music, the tone gets real goodLet me know when we"re gettin" closeYou can slide on out, or we can head on down the roadCause I"m a country boy, I"ve got a 4 wheel drivePile in my bed, I can take ya for a rideUp city streets, down country roadsI can get ya where you need to goCause I"m a country boyBucket seats, soft as baby"s new buttLockin" hubs, that"ll take ya through a deep rutCause I"m a country boy, I"ve got a 4 wheel drivePile in my bed, I can take ya for a rideUp city streets, down country roadsI can get ya where you need to goCause I"m a country boyx2http://music.baidu.com/song/59327418
2023-07-23 18:59:191

American Boy (Live Acoustic) 歌词

歌曲名:American Boy (Live Acoustic)歌手:VersaEmerge专辑:Live Acoustic EpМираж-American BoyIRC:ФАНЬ ЧАНЮЙЯ играю на балалайкеЭто самый русский инструментЯ мечтаю жить на ЯмайкеНа Ямайке балалаек нетИ нету счастья в личной жизниПроходят зря мои годаНу где ж ты,принц мой заграничный?Приходи поскорей, я жду тебя!American Boy, American JoyAmerican boy for allways timeAmerican boy, уеду с тобойУеду с тобой, Москва - прощай!American boy !Уеду с тобой !American boyЯ простая,русская девчонкаЗаграницей с роду не былаТы,Американец, ты мальчонкаУвози меня - и все дела!Я буду плакать и смеятьсяКогда усядусь в МерседесИ буду в роскоши купатьсяПриезжай поскорей за мной - я здесьAmerican Boy, American JoyAmerican boy for allways timeAmerican boy, уеду с тобойУеду с тобой, Москва - прощай!American boy !Уеду с тобой !American American American boyAmerican Boy, American JoyAmerican boy for allways timeAmerican boy, уеду с тобойУеду с тобой, Москва - прощай!American boy !Уеду с тобой !American Boy, American JoyAmerican boy for allways timeAmerican boy, уеду с тобойУеду с тобой, Москва - прощай!American boy !Уеду с тобой !American American American boyhttp://music.baidu.com/song/12514083
2023-07-23 18:59:391

Playing With The Big Boys 歌词

歌曲名:Playing With The Big Boys歌手:Martin Short&STEVE MARTIN专辑:The Prince Of EgyptArtist: Steve Martin / Martin Short LyricsSong: Playing with the Big Boys LyricsHotep and HuyBy the power of Ra...Mut...Nut...Khnum...Ptah...Nephthys...Nekhbet...Sobek...Sekhment...Sokar...Selket...Reshpu...Wadjet...Anubis...Anukis...Seshmu...Meshkent...Hemsut...Tefnut...Heket...Mafdet...Ra...Mut...Nut...Ptah...Hemsut...Tefnut...Sokar...Selket...Seshmu...Reshpu...Sobek...Wadjet...Heket...Mafdet...Nephthys...Nekhbet...Ra...So you think you"ve got friends in high placesWith the power to put us on the runWell, forgive us these smiles on our facesYou"ll know what power is when we are doneSon...You"re playing with the big boys nowPlaying with the big boys nowEv"ry spell and gestureTells you who"s the best, you"rePlaying with the big boys nowYou"re playing with the big boys nowYou"re playing with the big boys nowStop this foolish missionWatch a true magicianGive an exhibition howPick up your silly twig, boyYou"re playing with the big boys now!By the power of RaMut, Nut, Khnum, PtahSobek, Sekhmet, Sokar, SelketAnumbis, AnukisHemsut, Tefnut, Meshkent, Mafdet...You"re playing with the big boys nowYou"re playing with the big boys nowBy the might of HorusYou will kneel before usKneel to our splendorous power...You put up a frontYou put up a fightAnd just to show we feel no spiteYou can be our acolyteBut first, boy, it"s time to bow(Kowtow!)Or it"s your own grave you"ll dig, boyYou"re playing with the big boys nowPlaying with the big boysNow!http://music.baidu.com/song/8052613
2023-07-23 18:59:461

tell time什么意思

报时
2023-07-23 18:59:576

Smile的《Boys》 歌词

歌曲名:Boys歌手:Smile专辑:SmileBoysSmileboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyhot summer time always something on my minda kind of magic in the airI"m on the run to a sunny paradisewith crowed beaches everywhereI"m just a girl who"s looking for some funit"s time to get things started so common everybodyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyhot summer night always makes me loose controlit feels like heavennow I"m free and all the boysif you want to feel the heatjust come along and play with meI"m just a girl who"s looking for some funit"s time to get things started so common everybodyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyboys boys be my boyboys boys be my boyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyboys boys be my boyI wanna feel your bodyboys boys be my boytonight I"m ready honeyboys boys be my boyI wanna be your lover girlbe my lover boyhttp://music.baidu.com/song/2747315
2023-07-23 19:00:111

一首英语歌我忘记歌名了,但是是女生唱的!其中一句歌词boy met girl , girl leaves boy。大家帮帮忙

quitter
2023-07-23 19:00:193

do you havefun是什么意思

xkndjdkdj司机阿胶诶嗯哇矿务局我那看哇
2023-07-23 18:54:473

平原上的摩西库村是什么地方

傅东心离婚后去的边远村庄。在《平原上的摩西》中,傅东心与丈夫李守廉离婚后远走去了库村,一个偏远小村庄,一切都很任性。《平原上的摩西》是由张大磊执导,董子健领衔主演,海清友情出演,于2023年1月16日上线爱奇艺,该剧根据双雪涛同名小说改编,在跨越两个时代的奔流中,一桩未决的罪案浮现,打破了小城的平静。
2023-07-23 18:54:481

常见格式——sam

SAM(The Sequence Alignment / Map format) 格式,即序列比对文件的格式, 详细介绍文档: http://samtools.github.io/hts-specs/SAMv1.pdf SAM文件由两部分组成,头部区和主体区,都以tab分列。 头部区:以"@"开始,体现了比对的一些总体信息。比如比对的SAM格式版本,比对的参考序列,比对使用的软件等。 主体区:比对结果,每一个比对结果是一行,有11个主列和一个可选列。 随着Illumina/Solexa, AB/SOLiD and Roche/454测序技术不断的进步,各种比对工具产生,被用来高效的将reads比对到参考基因组。因为这些比对工具产生不同格式的文件,导致下游分析比较困难,因此一个通用的格式可以提供一个很好的接口用于链接比对与下游分析(组装,变异等,基因分型等)。因此SAM格式应运而生,主要是用来存储测序reads与参考序列比对结果信息的一种文件格式,以TAB为分割符,支持不同平台的短reads及长reads(最长为128Mbp)。 @HD VN:1.0 SO:unsorted (排序类型) 头部区第一行:VN是格式版本;SO表示比对排序的类型,有unknown(default),unsorted,queryname和coordinate几种。samtools软件在进行行排序后不能自动更新bam文件的SO值,而picard却可以。 @SQ SN:contig1 LN:9401 (序列ID及长度) 参考序列名,这些参考序列决定了比对结果sort的顺序,SN是参考序列名;LN是参考序列长度;每个参考序列为一行。 例如:@SQ SN:NC_000067.6 LN:195471971 @RG ID:sample01 (样品基本信息) Read Group。1个sample的测序结果为1个Read Group;该sample可以有多个library的测序结果,可以利用bwa mem -R 加上去这些信息。 例如:@RG ID:ZX1_ID SM:ZX1 LB:PE400 PU:Illumina PL:Miseq ID:样品的ID号 SM:样品名 LB:文库名 PU:测序以 PL:测序平台 这些信息可以在形成sam文件时加入,ID是必须要有的后面是否添加看分析要求 @PG ID:bowtie2 PN:bowtie2 VN:2.0.0-beta7 (比对所使用的软件及版本) 例如:@PG ID:bwa PN:bwa VN:0.7.12-r1039 CL:bwa sampe -a 400 -f ZX1.sam -r @RG ID:ZX1_ID SM:ZX1 LB:PE400 PU:Illumina PL:Miseq ../0_Reference/Reference_Sequence.fa ZX_HQ_clean_R1.fq.sai ZX_HQ_clean_R2.fq.sai ../2_HQData/ZX_HQ_clean_R1.fq ../2_HQData/ZX_HQ_clean_R2.fq 这里的ID是bwa,PN是bwa,VN是0.7.12-r1039版本。CL可以认为是运行程序@RG是上面RG表示的内容,后面是程序内容,这里的@GR内容是可以自己在运行程序是加入的 主体部分有11个主列和1个可选列 QNAME 比对的序列名称 例如:M04650:84:000000000-B837R:1:1101:22699:1759(一条测序reads的名称) FLAG Bwise FLAG(表明比对类型:paring,strand,mate strand等) 例如:99 RENAME 比对上的参考序列名 例如:NC_000075.6 POS 1-Based的比对上的最左边的定位 例如:124057649 MAPQ 比对质量 例如:60 CIGAR Extended CIGAR string(操作符:MIDNSHP)比对结果信息;匹配碱基数,可变剪接等 例如:87M MRNM 相匹配的另外一条序列,比对上的参考序列名 例如:= MPOS 1-Based leftmost Mate Position (相比于MRNM列来讲意思和POS差不多) 例如:124057667 ISIZE 插入片段长度 例如:200 SEQ 和参考序列在同一个链上比对的序列(若比对结果在负义链上,则序列是其反向重复序列,反向互补序列) 例如:ATTACTTGGCTGCT QUAL 比对序列的质量(ASCII-33=Phred base quality)reads碱基质量值 例如:-8CCCGFCCCF7@E- 可选的列 以TAG:TYPE:VALUE的形式提供额外的信息 (如果某一列为“0”或“*”表示这一列没有信息) 进行reads比对时通常表示reads的名字,如果这条reads比对到多条序列或比对到这条序列的多个位置,相同名字会出现多次。如果是pair-end reads,相同名字会出现2次,分别表示来自于R1文件的reads和R2文件的reads,如果其matepair reads也比对2个位置,也会出现2次,则相同名字共出现4次,如果一条reads也比对2个位置,则其matepair比对1个位置,则共出现3次,如果其matepair reads没有比对上序列也会出现1次(第三列显示“*”),所以pair-end测序,R1文件和R2文件同时mapping,相同reads的id最少出现2次。 数值结果如下: 1(1)该read是成对的paired reads中的一个 2(10)paired reads中每个都正确比对到参考序列上 4(100)该read没比对到参考序列上 8(1000)与该read成对的matepair read没有比对到参考序列上 16(10000)该read其反向互补序列能够比对到参考序列 32(100000)与该read成对的matepair read其反向互补序列能够比对到参考序列 64(1000000)在paired reads中,该read是与参考序列比对的第一条 128(10000000)在paired reads中,该read是与参考序列比对的第二条 256(100000000)该read是次优的比对结果 512(1000000000)该read没有通过质量控制 1024(10000000000)由于PCR或测序错误产生的重复reads 2048(100000000000)补充匹配的read 具体的flag值的解释,可以参考samtools软件提供的结果 samtools(Version: 1.3.1) 其中的 samtools flags 用法可提供flag值的查找结果 About: Convert between textual and numeric flag representation Usage: samtools flags INT|STR[,...] 例如: samtools flags 10 0xa 10 PROPER_PAIR,MUNMAP (10=2+8) samtools flags 12 0xc 12 UNMAP,MUNMAP (12=4+8) 具体的flag值的解释,也可参考如下网站: https://broadinstitute.github.io/picard/explain-flags.html 或者在必应当中搜索flag sam点击Explain SAM Flags-GitHub Pages进入该网页,也可以输入组合flag数值会出现所存在的意思 表示read比对的那条序列的序列名称(名称与头部的@SQ相对应),如果这列是“*”,可以认为这条read没有比对上的序列,则这一行的第四,五,八,九 列是“0”,第六,七列与该列是相同的表示方法 表示read比对到RNAME这条序列的最左边的位置,如果该read能够完全比对到这条序列(CIGAR string为M)则这个位置是read的第一个碱基比对的位置,如果该read的反向互补序列比对到这条序列,则这个位置是read的反向互补序列的第一个碱基比对的位置,所以无论该read是正向比对到该序列,或是其反向互补序列比对到该序列,比对结果均是 最左端的比对位置 表示为mapping的质量值,mapping Quality, It equals -10log10Pr{mapping position is wrong}, rounded to the nearest integer, A value 255 indicates that the mapping quality is not available. 该值的计算方法是mapping的错误率的-10log10值,之后四舍五入得到的整数,如果值为255表示mapping值是不可用的,如果是unmapped read则MAPQ为0,一般在使用bwa mem或bwa aln(bwa 0.7.12-r1039版本)生成的sam文件,第五列为60表示mapping率最高,一般结果是这一列的数值是从0到60,且0和60这两个数字出现次数最多 CIGAR string,可以理解为reads mapping到第三列序列的mapping状态, 对于mapping状态可分为以下几类: M:alignment match (can be a sequence match or mismatch) 表示read可mapping到第三列的序列上,则read的碱基序列与第三列的序列碱基相同,表示正常的mapping结果,M表示完全匹配,但是无论reads与序列的正确匹配或是错误匹配该位置都显示为M I:insertion to the reference 表示read的碱基序列相对于第三列的RNAME序列,有碱基的插入 D:deletion from the reference 表示read的碱基序列相对于第三列的RNAME序列,有碱基的删除 N:skipped region from the reference 表示可变剪接位置 P:padding (silent deletion from padded reference) S:soft clipping (clipped sequences present in SEQ) H:hard clipping (clipped sequences NOT present in SEQ) clipped均表示一条read的序列被分开,之所以被分开,是因为read的一部分序列能匹配到第三列的RNAME序列上,而被分开的那部分不能匹配到RNAME序列上。 “=”表示正确匹配到序列上 “X”表示错误匹配到序列上 而H只出现在一条read的前端或末端,但不会出现在中间,S一般会和H成对出现,当有H出现时,一定会有一个与之对应的S出现 例如: 162M89S 162H89M 149M102S 149H102M 40S211M 20M1D20M211H S可以单独出现,而H必须有与之对应的S出现时才可能出现,不可在相同第一列的情况下单独出现 N:如果是mRNA-to-genome,N出现的位置代表内含子,其它比对形式出现N时则没有具体解释 M/I/S/=/X:这些数值的加和等于第10列SEQ的长度 这条reads第二次比对的位置,在利用bwa mem产生sam文件时,如果该列是“ ”而 第3列RNAME不是“ ”则表示该reads比对到第3列显示序列名的序列上,而没有比对到其他位置,在利用bwa aln及bwa sampe比对生成的sam文件,如果和上述情况相同,则第7列为“=”,上述情况均表示该reads只比对到这一个位置 如果第3列RNAME和第7列MRNM都为“*”,则说明这条reads没有匹配上的序列,如果这条reads匹配两个序列,则第一个序列的名称出现在第3列,而第二个序列的名称出现在第7列 该列表示与该reads对应的mate pair reads的比对位置,如果这对pair-end reads比对到同一条reference序列上,在sam文件中reads的id出现2次,Read1比对的第4列等于Read2比对的第8列。同样Read1比对的第8列等于Read2比对的第4列。例如: 第1列(Read id)····第4列(Read1比对位置)····第8列(mate-pair reads比对位置) 22699:1759····124057649····124057667 22699:1759····124057667····124057649 相同的reads id一个来自Read1文件,一个来自Read2文件,第4列和第8列是对应的 TLEN:signed observed Template LENgth (可以理解为文库插入片段长度) 如果R1端的read和R2端的read能够mapping到同一条Reference序列上(即第三列RNAME相同),则该列的值表示第8列减去第4列加上第6列的值,R1端和R2端相同id的reads其第九列值相同,但该值为一正一负,R1文件的reads和R2文件的reads,相同id的reads要相对来看。在进行该第列值的计算时,如果取第6列的数值,一定要取出现M的值,S或H的值不能取。 the unisgned observed template length equals the number of base from the leftmost mapped base to the rightmost mappedbase. Theleftmost segment has a plus sign and the rightmost has a minus sign
2023-07-23 18:54:451

智慧职教里面考试为啥进不去

智慧职教里面考试进不去是因为网络上网高峰引起的。智慧职教是一款职业教育和智慧教育的软件,当使用的人数过多,会提示网络上网高峰引起的,暂时不能使用字样。会导致无法正常使用软件,考试也进不去。所以智慧职教里面考试进不去是因为网络上网高峰引起的。
2023-07-23 18:54:441