west

可能的原因有， maker加的量少，半干法转膜的时候转缓液加多了，maker没有保存好，如长期放在室温分解了，也可能是跑电泳的胶的问题。跑胶的时候电压越小条带相对弥散些，不过还不至于maker不清楚。

首先要明白，marker和内参不是一种东西。Marker是用来标定蛋白或者DNA大小的。内参对于哺乳动物细胞表达来说一般是指由管家基因编码表达的蛋白，它们在各组织和细胞中的表达相对恒定，在检测蛋白的表达水平变化时常用它来做参照物。其作用是校正蛋白质定量、上样过程中存在的实验误差，保证实验结果的准确性。pcr产物检测一般marker就可以了。不用内参。western一般全都要用。免疫组化检测蛋白偏重于蛋白在细胞及组织中的表达分布，定量较为不精确。所以要根据你实验的目的选择实验方法。

在Western实验中，蛋白Marker可以监控蛋白质在SDS-PAGE中的迁移，监控蛋白的转膜效率，确定蛋白分子量大小。选择正确的蛋白Marker是western blot实验成功的必备条件之一。蛋白Marker分为两种：一种是未预染的Marker，即宽分子量蛋白标准，高分子量蛋白标准及低分子量蛋白标准；另一种是预染的Marker，即单色预染和多色预染。 一、未预染蛋白Marker 未预染的蛋白Marker是最简单，也是最准确的一种，由于没有附带染料分子或者标记大小正好是蛋白原本的大小，是精确判断蛋白大小必须的。现在的Marker多数选用预混合的marker，方便不同大小蛋白的比较。 二、预染蛋白Marker 预染蛋白Marker是一些纯化较好的蛋白混合物，通过与染料共价偶联，在电泳过程中或者转膜时可以直接观察到。预染Marker的出现方便了我们的实验，这种蛋白分子量标准可以帮助我们在电泳时，电泳后，以及转膜后检测电泳情况和估计迁移率。值得注意的是预染蛋白Marker与染料共价偶联，所以在不同的缓冲条件下电泳时迁移特性可能会发生某些变化，可能导致一些偏差，所以不太适合精确定位蛋白。 同科生物现货供应即用型的蛋白Marker，无需煮沸可以直接上样，而且条带尖锐，颜色稳定，转膜效率高。

她的扮演者叫Lauren German，1978年11月29日生于加利福尼亚州奥兰治县亨廷顿滩市(Huntington Beach)出演过的影视剧及扮演的角色Down to You (2000) as Lovestruck Woman Undressed: Season 3 (2000) as Kimmy Shotgun Love Dolls: "Pilot" (2001) as Beth 7th Heaven: "Apologize" (2001) as Marie Going to California: "A Little Hard in the Big Easy" (2002) as Tiffany A Walk to Remember (2002) as Belinda Dead Above Ground (2002) as Darcy Peters A Midsummer Night"s Rave (2002) as Elena The Lone Ranger (2003) as Emily Landry The Texas Chainsaw Massacre (2003) as Teenage Girl u211e: aka Simple Lies (2005) as Melissa Standing Still (2005) as Jennifer Sex, Love & Secrets: episodes 1–8 (2005) as Rose Piggy Banks: aka Born Killers (2005) as Gertle Surrender, Dorothy (2006) as Maddy It Is Fine. Everything Is Fine! (2007) as Ruth Spin (2007) as Cassie Love and Mary (2007) as Mary Hostel: Part II (2007) as Beth What We Do Is Secret (2007) as Belinda Carlisle Mating Dance (2008) as Abby Made for Each Other (2009) as Catherine Dark Country (2009) as Gina Happy Town: all episodes (2010) as Henley Human Target: "Kill Bob" (2011) as Angie/Natalia The Divide (2011) as Eva Memphis Beat: two episodes (2011) as Kaylee Slater (Mark"s Neighbor) Hawaii Five-0 (2011) as Lori Weston

歌曲名:Lucifer (Produced By Kanye West)歌手:Jay-Z 专辑:Black AlbumSee, See, SeeI"m from the murder capitol where we murder for capitolSee, See, SeeKanyeeze you did it again you a genius niggaSo you niggas change your attitude "for they askin what happened to youLord forgive himHe got them dark forces in himBut he also got a rightous cause for sinninThem a murder me so i gotta murder them firstEmergency doctors performin proceduresJesusI ain"t tryin to be facetiousBut "Vengance is mine" said the LordYou said it better than allLeave niggas on deaths doorBreathin off res-por-ratorsfor killin my best boy, HATERSOn perminate hiatus as i skateIn the Mabach BenzFly this as i"m latePumpin "Brown Sugar" by D"AngeloIn Los AngelesLike an evangelistI can introduce you to your makerBring you closer to natureAshes after they cremate you bastardsHope you been readin your psalms and chaptersPayin your ties being good CatholicsI"m comminSee, See, SeeI"m from the murder capitol where we murder for capitolSee, See, SeeSo you niggas change your attitude "for they askin what happened to youYesThis is Holly warI wet cha all with the Holly waterSpray from the Hetckler Koch autoMatic all the static shall cease to existLike a sematical i throw a couple at youTake sixSpread love to all of my dead thugsI"ll pour out a little Louie til i head aboveYes SirAnd when i perishThe meek shall inherit the earthUntil that time it"s on a poppin ChurchLike Don BishopThe fifth upon cock eitherLift up your soul or give the Holly ghost pleaseI leave ya in somebodys CatedrialAnd stuntin like Evil KenevilI"ll let you see where that bright light lead youThe more you talk the more you irkin usThe more you gonna need memorial servicesThe Black Albums second verse is likeDevil"s Pie please save some dessert for usMan i gotta get my soul rightI gotta get these Devils out my lifeThese cowards gonna make a nigga rideThey won"t be happy til somebody diesMan i gotta get my soul right"For i"m locked up for my whole lifeEvertime it seems it"s all rightSomebody want they soul to rise(I"ll chase you off of this Earth)I got dreams of holdin a Nine millaTo Bobs killaAskin him "why?" as my eyes fill upThese days i can"t wake up with a dry pillowGone but not forgotten holmes i still feel yaSO...Curse the day that birthed the bastardWho caused your Church massReverse the crashReverse the blastAnd reverse the carReverse the day, and there you areBob AllahLord forgive him we all have sinedBut Bobs a good dude please let him inAnd if you feel in my heart that i long for revengePlease blame it on the sun of the mourninThanks Againhttp://music.baidu.com/song/1257503

2.Poet"s Heart作词：Ruth - Anne Cunningham、John Shanks作曲：Ruth - Anne Cunningham、John ShanksWhen did I lose my voice? 我什麼时候无话可说When did I make the choice to beliving so easy in the quiet? 我什麼时候选择了在安静中如此简单的生活Too much of restless thinking 太多的无休止的想法But they say time is everything. 但他们说时间就是全部You came and opened up the slience. 你来到并打破了寂静So I lie awake, 所以我保持清醒writing symphonies trapped inside me, 写下萦绕在我心头的旋律But now I know, what you give to me, 但现在我知道，你给我了什麼东西I"m living with the poet"s heart, heart 我有了一颗诗人的心Now all the lights turned on and I"m never gonna stop 现在所有的灯都打开了，我也决不会停止As the words come out, it"s because you lit the spark. 现在所有的词语都出现了，因为是你点燃了星火You"re a priceless, timeless, precious work of art. 你是无价的，无限的，珍贵的艺术品Though everywhere you are, 不管你在哪里，I"m living with the poet"s heart. 我有了一颗诗人的心Whisper to me again 在此对我耳语You *fall* from my ears straight to my pen,你从我的耳边到了我的笔头How did you see right through? 你如何看透It"s like you"re always new. 你好像永远都是展新的So I lie awake 所以我保持清醒writing symphonies, you awoken me 写下你唤醒我的旋律，Cause now I know, what you sing to me, 因为我现在知道，你对我唱了什麼I"m living with the poet"s heart, heart. 我有了一颗诗人的心现在所有的灯都打开了，我也决不会停止As the words come out, it"s because you lit the spark. 现在所有的词语都出现了，因为是你点燃了星火You"re a priceless, timeless, precious work of art. 你是无价的，无限的，珍贵的艺术品Though everywhere you are, 不管你在哪里，I"m living with the poet"s heart. 我有了一颗诗人的心Oh, ohSo let"s lie awake, making history 所以我们保持清醒，创造历史Yeah, you and me 你和我Cause now we know,因为我们现在知道so won"t you sing to me, 所以难道你不唱给我吗our melody,我们的旋律yeah现在所有的灯都打开了，我也决不会停止As the words come out, it"s because you lit the spark. 现在所有的词语都出现了，因为是你点燃了星火You"re a priceless, timeless, precious work of art. 你是无价的，无限的，珍贵的艺术品Though everywhere you are, 不管你在哪里，I"m living with the poet"s heart. heart, heart, heart我有了一颗诗人的心Now my feet won"t touch the ground, 现在我的双脚不会再著地Yeah, we"re flying high homeward bound 是的，我们在飞往高处的家的地方Take my hand, yeah, just like that.拉著我的手，是的，就像这样，We"ll keep riding, riding 我们继续骑行，骑行Don"t look back, don"t look back,别回头，别回头no, no...英文是对的。。中文是我自己尽量的，没什麼文笔。。。。

正确答案是what

natwest要申请网上银行，在本地银行申请

<%@ Register Assembly="Coolite.Ext.Web" Namespace="Coolite.Ext.Web" TagPrefix="ext" %><!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><html xmlns="http://www.w3.org/1999/xhtml" ><head runat="server"> <title></title> <script type="text/javascript"> function GetTree(node) { var eIdtd = document.getElementById("txtTitle"); var enumPare = document.getElementById("txtContent"); eIdtd.innerHTML ="编号："+ node.id; enumPare.innerHTML ="内容："+ node.text; } </script> </head><body> <form id="form1" runat="server"> <div> <ext:ScriptManager ID="ScriptManager1" runat="server"> </ext:ScriptManager> <ext:ViewPort ID="ViewPort1" runat="server"> <Body> <ext:BorderLayout ID="BorderLayout1" runat="server"> <North MarginsSummary="5 5 5 5"> <ext:Panel AllowDomMove="true" ID="Paneles" Height="250" runat="server" Title="top" Icon="Information" Header="true" Collapsed="true" Collapsible="true"> <Body> <div style="float:left; white-space:normal; padding-left:5px; width:50%; vertical-align:middle; "><p> 导航：这是上边！ </p></div> </Body> </ext:Panel> </North> <West MinWidth="225" MaxWidth="400" Collapsible="true" Split="true" MarginsSummary="0 0 0 0" CMarginsSummary="0 0 0 0"> <ext:TreePanel ID="treePark" Header="false" Frame="false" Title="Tree" Width="300" Lines="false" BodyBorder="false" RootVisible="false" runat="server"> <Root> <ext:TreeNode Text="Composers" Expanded="true"> <Nodes> <ext:TreeNode Text="Beethoven" Icon="UserGray"> <Nodes> <ext:TreeNode Text="Concertos"> <Nodes> <ext:TreeNode NodeID="1" Text="No. 1 - C" Icon="Music" /> <ext:TreeNode NodeID="2" Text="No. 2 - B-Flat Major" Icon="Music" /> <ext:TreeNode NodeID="3" Text="No. 3 - C Minor" Icon="Music" /> <ext:TreeNode NodeID="4" Text="No. 4 - G Major" Icon="Music" /> <ext:TreeNode NodeID="5" Text="No. 5 - E-Flat Major" Icon="Music" /> </Nodes> </ext:TreeNode> <ext:TreeNode Text="Quartets" > <Nodes> <ext:TreeNode NodeID="6" Text="Six String Quartets" Icon="Music" /> <ext:TreeNode NodeID="7" Text="Three String Quartets" Icon="Music" /> <ext:TreeNode NodeID="8" Text="Grosse Fugue for String Quartets" Icon="Music" /> </Nodes> </ext:TreeNode> <ext:TreeNode Text="Sonatas" > <Nodes> <ext:TreeNode Text="Sonata in A Minor" Icon="Music" /> <ext:TreeNode Text="sonata in F Major" Icon="Music" /> </Nodes> </ext:TreeNode> </Nodes> </ext:TreeNode> </Nodes> </ext:TreeNode> </Root> <Listeners> <Click Handler="Javascript:GetTree(node);" /> </Listeners> </ext:TreePanel> </West> <Center> <ext:Panel ID="panel1" Height="500px" BodyBorder="false" Frame="false" AutoScroll="true" runat="server"> <Body> <table cellpadding="0" cellspacing="0" width="98%" border="0"> <tr> <td height="20" colspan="2" > <span style=" font-size:xx-large; text-align:center "> 这是中间部位 </span> </td> </tr> <tr> <td width="20" height="20" rowspan="2" align="center" valign="top"> </td> <td style="height: 50px; vertical-align: bottom; overflow: scroll;" align="center"> <asp:Label ID="txtTitle" runat="server"></asp:Label> </td> </tr> <tr> <td align="left" id="txtContent1"> <asp:Label ID="txtContent" runat="server"></asp:Label> </td> </tr> </table> </Body> </ext:Panel> </Center> </ext:BorderLayout> </body> </ext:ViewPort> </div> </form></body></html>这个直接建一个页面，粘贴进去就可以用了！

dale road worthing west sussex bn11 2bh united kingdom戴尔路值得英国西苏塞克斯bn11 2bh英国

[Hook x4]Lamborghini MercyYour chick she so thirstyI"m in that two seat LamboWith your girl she tryna jerk me[Big Sean]Drop it to the floorMake that *ss shakeWoah make the ground move, that"s an *ss quakeBuilt a house up on it, that"s an *ss stateRoll my blunt on it, that"s an *ss traySay Ye, say Ye, don"t we do this err" day-day?I work them long nights, long nights to get a pay dayFinally got paid, now I need shade and a vacayAnd n*ggas still hatin", so much hate I need an AKNow we out in Paris, yeah I"m Perrierin"White girls politicin" that"s that Sarah PalinGettin", Californicatin"I give her that D, cause that"s what"s born and raised in[Hook][Pusha T]It"s prime time, my top back, this pimp gameI"m red leather, this , I"m Rick JamesI"m bill droppin", Ms. Pacman is pill poppin"This pill popped, I"m poppin" tooThese blue dolphins need two coffinsAll she want is some heel moneyAll she need is some bill moneyHe take his time, he counts it outI weighs it up, that"s real moneyCheck the neck, check the wristThem heads turnin", that"s exorcistMy Audemar like Mardi GrasThat"s Swiss time and that"s excellenceTwo door preferenceRoof gone George JeffersonThat white frost on that pound cakeSo your Duncan Heinz is irrelevantLambo, Mercy-lago, she go wherever I goWherever we go we do it pronto[Hook][Kanye West]Let the suicide doors upI threw suicides on the tour busI threw suicides on the private jetYou know what that mean, I"m fly to deafI step in Def Jam building like I"m the sh*tTell ‘em “give me fifty million or I"mma quit”Most rappers taste level ain"t at my waist levelTurn up the bass "til it"s up in your face levelDon"t do no press but I do the most press, kidPlush out my-my , make yours look like PreciousTalkin" "bout Mary she gone off that MollyNow the whole party is melted like DhaliNow everybody is movin" they bodyDon"t sell me apartment, I move in the lobbyn*ggas is loiterin" just to feel importantYou gon see lawyers and n*ggas ? gettin Jordans[2 Chainz]Can"t ? to my campaignCoupe the color of mayonniaseI"m drunk and high at the same timeDrinkin" champaign on the airplaneSpit rounds like the gun rangeBeat it up like a rampage100 bands, country girl now your girl need a bandaidGrade A, A1, chain the color of AkonPlatinum diamonds backpack around meCosigned by Louis VouttonHorse power, horse powerAll this Polo on I got horse power? wrist cost 4 thousandI make it rain, she want more showersRain pourin", all my cars is foreignAll my broads is foreign, money tall like Jordan[Hook]From.Lin Bros

歌曲名:Hey Mamma歌手:Westernhagen (Wea)专辑:WesternhagenCam"ron - Hey MaHey ma, what"s up, lets slide, all right, all rightAnd we gon get it on tonightYou smoke, I smoke, I drink, me too, well goodCause we gon get high tonightGot drops, got Coups, got Trucks, got juice, all rightCause we gon take a ride tonightSo ma, what"s up, let"s slide, all right, all rightAnd we gon get it on tonightYo, Now I was downtown clubbin", ladies nightSeen shorty she was crazy rightAnd I approach baby likeMa, What"s your age and type?She looked at me and said use a baby rightI told her, I"m 18 and live a crazy lifePlus I"ll tell you what the 80"s likeAnd I know what ladies likeNeed a man that"s polite, listens and takes adviceI could be all three, plus I can lay the pipeCome with me come stay the nightShe looked at me laughin", like boy your game is tightI"m laughin" back like show you rightGet in the CarAnd don"t touch nothing Sit in the carLet discuss Somethin"Either we lovin" or I"ll see you tommorowNow we speeding up the WestsideHand creepin" up her left side, I"m ready to do itReady to bone, ready for dome55th exit, damn, damn, already we homeNow let"s get it onNow That I got a girl, my Ex wanna holla and spitTold me to acknowledge her quickShe like Cam stop frontin"On that Dave Hollister TipCome over lets swallow and sipI"m like momma that"s itI promise you dick, usually have a problem with chicksThey all say I"m rotten and richBut not her, booby"s realHigh heel dooby feel, plus got them Gucci nails, uhYou a cutie still, and this my down girl tooAin"t no groupie dealWe left the movies with Uzies, Suzuki wheels,To the Jocose, I tell you my booby"s realI mean she do be winning, luey spinninGo to the crib she got the Gucci linen"I see booby grinningShe looked and said Cam, I know that you be sinningNaw, I"m a changed man, look at the range maimI got a whole new game planLooked and said that"s nothing but game CamShe was right; she was up in the Range manDroped her off at the L, now I"m flippin" the cellThat"s right I had to call up LYou L, what up, I hit, what else, plus dome, say wordAnd we got it on tonighthttp://music.baidu.com/song/7403567

western显色的方法主要有以下几种：i.放射自显影ii.底物化学发光ECLiii.底物荧光ECFiv.底物DAB呈色现常用的有底物化学发光ECL和底物DAB呈色，体同水平和实验条件的是用第一种方法，目前发表文章通常是用底物化学发光ECL。只要买现成的试剂盒就行，操作也比较简单，原理如下（二抗用HRP标记）：反应底物为过氧化物+鲁米诺，如遇到HRP，即发光，可使胶片曝光，就可洗出条带。

Kanye West Flashing Lights Lyrics (intro)Flashing LightsFlashing LightsFlashing LightsFLashing Lights(Verse 1)She dont belive in shooting starsbut she belives in shoes and carsWood floors in the new apartmentCouture from the store"s departmentYou more like love to start s**tIm more of da, trips to floridaorda the orderves, views of da waterstrait from a page of your favorite authorAnd the weather is so breazyman why cant life always be this eazyshe in the mirror dancing so sleazyI get a call, like where are you deazyI tried to hit you with the o wokietill i got flash by the popuratzidang these niggas got mei hate these niggas more than nazis(chorus)As i recall I know you love to show offBut I never thought that you would take it this farBut what do i knowFlashing LightsWhat do I knowFlashing Lights(verse 2)[Flashing Lights lyrics on http://www.metrolyrics.com]I know its been a while sweetheart, we hardly talkI been doing my thangi know it was style babay a bay lately, u been all on my brainand if somebody would of told me a month ago fronten thoYo i wouldn"t wanna knowif somebody would of told me a year ago it a go would get this difficultfeelin like katrina with no femalike martin with no ginalike a flight with no visafirst class with the seat back i still see yain my past you on the otherside of the glassof my memories museumim just sayinhey Mona Lisacome home you know you cant Rome without Ceasar(chorus)as i recall I know you love to show offbut i never thought that you would take it this farbut what do i knowflashing lightswhat do i knowflashing lights(chorus)as you recall you know i love to show offbut you never thought that i would take it this farbut what do you knowflashing lightswhat do you knowflashing lightsflashing lightsflashing lights

外国女孩儿的名字，有值得爱的意思

歌曲名:Mandy歌手:Westlife专辑:Forever Friends - Mum In A MillionMandywestlifeI remember all my lifeRaining down as cold as iceShadows of a manA face through a windowCrying in the nightThe night goes intoMorning, just another dayHappy people pass my wayLooking in their eyesI see a memoryI never realizedHow happy you made me oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyI"m standing on the edge of timeI"ve walked away when love was mineCaught up in a world of uphill climbingTears are on my mindAnd nothing is rhyming, oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyYesterday"s a dream I face the morningCrying on a breezeThe pain is calling, oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyYou came and you gave without takingBut I sent you away, oh MandyYou kissed me and stopped me from shakingAnd I need youOh Mandy won"t you listen to what I"ve got to sayOh baby don"t let me throw it all it awayOh Mandy won"t you listen to what I"ve got to sayAnd I need you today...Oh Mandy...http://music.baidu.com/song/7500743

歌曲名:Mandy歌手:Westlife专辑:Greatest HitsMandywestlifeI remember all my lifeRaining down as cold as iceShadows of a manA face through a windowCrying in the nightThe night goes intoMorning, just another dayHappy people pass my wayLooking in their eyesI see a memoryI never realizedHow happy you made me oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyI"m standing on the edge of timeI"ve walked away when love was mineCaught up in a world of uphill climbingTears are on my mindAnd nothing is rhyming, oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyYesterday"s a dream I face the morningCrying on a breezeThe pain is calling, oh MandyWell you came and you gave without takingBut I sent you away, oh MandyWell you kissed me and stopped me from shakingAnd I need you today, oh MandyYou came and you gave without takingBut I sent you away, oh MandyYou kissed me and stopped me from shakingAnd I need youOh Mandy won"t you listen to what I"ve got to sayOh baby don"t let me throw it all it awayOh Mandy won"t you listen to what I"ve got to sayAnd I need you today...Oh Mandy...http://music.baidu.com/song/12486402

cyriack去皮机、、作于西敏寺桥上

十一位数。西太平洋银行是新西兰的第二大银行，英文名称是Westpac，也就是WestPacific西太平洋的混合连读词；它是一家总部设在澳大利亚悉尼的澳洲银行和金融服务提供商。新西兰境内经营的西太平洋银行是澳洲的子公司。在澳洲西太平洋银行拥有最多的商业网点和自动提款机，如果以管理资产数量来计算的话Westpac是澳洲第二大银行。

U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955U0001f955

NIX的老婆是爱尔兰总理的女儿

西域男孩.不过我不知道对不对.好像是西域刚刚出来一本这个专集.

Nicky 中国歌迷中喜欢叫他 美人 因其美若天仙

美人nicky

nicky:if i had to live my life without you near methe days would all be emptythe nights would seem so longwith you i see forever oh so clearlyi might have been in love beforebut it never felt this strong第一段开头是nico的kian:if the road ahead is not so easyour love will lead a way for uslike a guiding starkian:you don"t have change a thingi love you just the way you are第二段开头是ki的，中间插了一点集体的《Nothong is gonna change my love for you》是2006年的，因为这首歌是翻唱的，所以没有MV，或者在演唱会上唱过

Brian McFadden、Mark Feehily、Nicky Bryne、Shane Filan、Kian Egan

nicky~~~~负责和声的~。那个时候好嫩啊~

除了基本的 你还想了解什么

nicky 开放分类： 明星、歌手 Nicky全名: Nicholas Bernard James Adam Byrne 中文译名: 尼基·柏恩 小名: Nico Nix 出生日期: 1978年10月9日 星座: 天平座 身高: 176厘米 眼睛颜色: 蓝色 头发颜色: 金色 家庭成员: 老婆Georgina 曾就读学校: Plunkit College 以前的工作: 利兹俱乐部的守门员 心中的英雄: Bruce Wills 最差的学科: 数学 最好的学科: 英语还有地理 爱好: 足球、唱歌 最喜欢做的事: 足球、在外面吃饭、购物、回爱尔兰看家人 最不喜欢做的事: 吸烟、肮脏和下流的人 喜欢的颜色: 蓝色还有白 喜欢的运动: 足球、诺克斯 (台球) 最喜欢的男演员: Bruce Willis 、 Brad Pitt 最喜欢的女演员: Demi Moore 、Cameron Diaz 最喜欢的男歌手: Phil Collins 最喜欢的女歌手: Natalie Imbroglio 最喜欢的影片: 《Titanic》《 Die Hard Armageddon》 最喜欢的食物: 日本料理 最喜欢的饮料: 百事可乐 最想住的地方: 都柏林的家里 最想去的地方: 加勒比和纽约、悉尼 最喜欢的香水: CK Eternity 最喜欢的球队: 曼联 最喜欢的乐队: Boyzone 最喜欢的歌曲: Flying Without Wings & Aganist All Odds 生活准则: 不认为别人比我优秀，也不认为我比别人优秀 口头禅: La Gra (爱尔兰语 大意是沐浴在爱中) 如果你是动物 你想做: 老虎 最想看见的人: PHIL COLLINS & MADONNA 喜爱的乐器: 钢琴 Nicky喜欢Gucci牌子的衣服 老婆是爱尔兰总理的千金 很喜欢Ronan 即使有机会也不要看女朋友的日记 讨厌坐电梯 宁可爬12层楼也不坐 Nicky有一对幸运袜 不论什么演出他都会穿哦 如果没有加入Westlife他会做警察 这样喜欢他的小孩都会避他 Nicky觉得这世界有外星人 样子就像Bryan 爱尔兰的歌迷长去找女朋友聊天 Nicky的妈妈会请歌迷们喝茶 都柏林出生的Nicky在音乐的环境中长大，爸爸尼可拉斯(Nicholas)是一个名叫Nikki And Studz的餐馆驻唱乐团的主唱，希望他儿子能成功的干掉他！Nicky说：“靠着音乐过活一直是我老爸的梦想，搞不好因为我们这个团，他现在有更多的场子可唱呢！”早在80年代，Nicky经常到位于Baldoyle一地的跑道旅店Racecourse Inn帮忙老爸架乐器，后来，男孩特区合唱团(Boyzone)的团员Shane、Keith、Ronan曾经来跑道旅店畅饮呢！虽然出身于音乐家庭，Nicky差点就成了足球国手，一度是Newcastle、Everton、Leeds等热门分区联队争相邀请加盟的选手，Nicky最后加盟Leeds联队，原本希望自己能被选为守门员，虽然有着五呎十吋的壮硕体型，球队还是觉得他太矮了!深感挫折之余，Nicky回到都柏林，一时之间不知道该做什么，曾经考虑加入爱尔兰的Gardai警察队。 Nicky 档案原名：Sura Teerakol艺名：Nicky出生地：泰国生日：1979年1月21日星座：水瓶座身高：179 cm　体重：71 kg血统：1/2泰国1/2中国家庭: 哥哥&妹妹宗教: 基督徒学历：美国加利福尼亚山地高中 曼谷Tonburi 大学职业：模特、歌手、演员语言：英语、泰语喜爱的汽车: 本田喜爱的颜色: 白色喜爱的品牌: Fubu 05喜爱的运动: 足球 橄榄球喜爱的足球队: 曼彻斯特喜爱的动物: 狗喜爱的节日：圣诞目标：全面国际艺人唱片:Nicky （2001）Thug Life （2002）Hip Hop in Love （2003）Hot （2004）Nicky Pimp （2005）电视剧:《Kratook Nuad Suah》《Nueng Fah Lanka Deiw》《Wai Son》广告:“SPY”冰酒“In Hand”Cologne“Euro”蛋糕电影:《子弹青少年》《Lub Lae Kon Mahasajun》《恶魔火车》《Narok Sao Tarutoa》《Sang Tai, Haam Tai》《Puchai Pai Leung》《Puchai See Muang》奖项:2000年泰国新秀大赛2003-2005年泰国最性感男明星2005年泰国“Rath ”报纸评选性感明星第5名

歌曲名:Survivors歌手:Matthew West专辑:The Story Of Your LifeNow that you"re out of my lifeI"m so much betterYou thought that I"d be weak without youBut I"m strongerYou thought that I"d be broke without youBut I"m richerYou thought that I"d be sad without youI laugh harderYou thought I wouldn"t grow without youNow I"m wiserThough that I"d be helpless without youBut I"m smarterYou thought that I"d be stressed without youBut I"m chillin"You thought I wouldn"t sell without youSold 9 millionI"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)I"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)Thought I couldn"t breathe withoutI"m inhalingYou thought I couldn"t see without youPerfect visionYou thought I couldn"t last without youBut I"m lastin"You thought that I would die without youBut I"m livin"Thought that I would fail without youBut I"m on topThought it would be over by nowBut it won"t stopThought that I would self destructBut I"m still hereEven in my years to comeI"m still gon be hereI"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)I"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)I"m wishin" you the bestPray that you are blessedBring much success, no stress, and lots of happiness(I"m better than that)I"m not gon blast you on the radio(I"m better than that)I"m not gon lie on you and your family(I"m better than that)I"m not gon hate on you in the magazines("m better than that)I"m not gon compromise my Christianity(I"m better than that)You know I"m not gon diss you on the internetCause my mama taught me better than thatI"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)I"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)Oh (oh) oh (oh)...After of all of the darkness and sadnessSoon comes happinessIf I surround my self with positive thingsI"ll gain prosperityI"m a survivor (What?)I"m not gon give up (What?)I"m not gon stop (What?)I"m gon work harder (What?)I"m a survivor (What?)I"m gonna make it (What?)I will survive (What?)Keep on survivin" (What?)http://music.baidu.com/song/2832392

方便观察电泳进行情况，确定电泳结束时间

请问这个手表多少钱

west end,也就是俗称的"瓦斯针"手表,是瑞士知名手表,创立于1886年。因为避震非常好，因此在二战时期，很多国家的军队将瓦斯针手表作为军表。战后也有很多军队沿用这个品牌的手表，在当时的条件下，瓦斯针手表避震出色，夜光强悍，有传言说瓦斯针手表的夜光，在夜间甚至能用来照明看清书籍上的文字。可见其夜光犀利。在上世纪50年代前后，在我国的军队中，一般也要一定级别的干部才能佩戴一块瓦斯针手表，在当时，佩戴瓦斯针手表是身份的象征，现在存世的一些瓦斯针的老手表，很大一部分都来自部队的老军人，老干部。过去五六十年了，估计表已经很旧了，但是有很好的收藏价值，可以好好保管，是很难得的藏品。

when I am down and, oh my soul, so weary; 当我消沉之时，我的灵魂无所依附When troubles come and my heart burdened be; 困难来袭之时，我的心不堪重负Then, I am still and wait here in the silence, 寂静如斯，我依然静静地在这等待Until you come and sit awhile with me. 直到你到我的身边You raise me up, so I can stand on mountains; 你鼓舞了我，我才能立于山巅You raise me up, to walk on stormy sea; 你鼓舞了我，我才能遨游怒海I am strong, when I am on your shoulders; 靠在你的肩上，我才变得坚强You raise me up... To more than I can be. 你鼓舞了我，让我超越极限You raise me up, so I can stand on mountains; You raise me up, to walk on stormy seas; I am strong, when I am on your shoulders; You raise me up... To more than I can be. You raise me up, so I can stand on mountains; You raise me up, to walk on stormy seas; I am strong, when I am on your shoulders; You raise me up... To more than I can be. You raise me up, so I can stand on mountains; You raise me up, to walk on stormy seas; I am strong, when I am on your shoulders; You raise me up... To more than I can be. You raise me up... To more than I can be.

youraisemeup-westlifewheniamdownand,ohmysoul,soweary;我消沉时灵魂如此疲惫whentroublescomeandmyheartburdenedbe;我隐入困境心灵不堪重负then,iamstillandwaithereinthesilence,孤寂中我只有静静等待untilyoucomeandsitawhilewithme.直到你出现坐在我的身边youraisemeup,soicanstandonmountains;你鼓舞了我我才能屹立在山顶youraisemeup,towalkonstormyseas;你鼓舞了我我才能在风大浪急的海面如履平地iamstrong,wheniamonyourshoulders;我依然强壮当有了你的肩头可依youraisemeup…tomorethanicanbe.你鼓舞了我我才能不断地超越youraisemeup,soicanstandonmountains;你鼓舞了我我才能屹立在山顶youraisemeup,towalkonstormyseas;你鼓舞了我我才能在风大浪急的海面如履平地iamstrong,wheniamonyourshoulders;我依然强壮当有了你的肩头可依youraisemeup…tomorethanicanbe.你鼓舞了我我才能不断地超越youraisemeup,soicanstandonmountains;你鼓舞了我我才能屹立在山顶youraisemeup,towalkonstormyseas;你鼓舞了我我才能在风大浪急的海面如履平地iamstrong,wheniamonyourshoulders;我依然强壮当有了你的肩头可依youraisemeup…tomorethanicanbe.你鼓舞了我我才能不断地超越youraisemeup,soicanstandonmountains;你鼓舞了我我才能屹立在山顶youraisemeup,towalkonstormyseas;你鼓舞了我我才能在风大浪急的海面如履平地iamstrong,wheniamonyourshoulders;我依然强壮当有了你的肩头可依youraisemeup…tomorethanicanbe.你鼓舞了我我才能不断地超越

太平洋西北部望采纳！谢谢！

东西南北的英文分别是east，west，south，north。表示方向的英语单词还有northeast，northwest，southeast，southwest等等。1、东east读音：英[iu02d0st]；美[iu02d0st]词义：adj.东方的；adv.向东方2、西west读音：英[west]；美[west]词义：n.西；西方；西部；西方国家；adj.西方的；西部的3、南south读音：英[sau028aθ]；美[sau028aθ]词义：n.南；南方；adj.南方的4、北north读音：英[nu0254u02d0θ]；美[nu0254u02d0rθ]词义：adj.北方的；北的；adv.向北方例句1、游客到达时搞不清东西南北了。The tourists were very mixed up when they arrived.2、方位基点就是东西南北。The cardinal points of the compass are north，south，east and west.

Ihavemystudies。同义句就是两个句子表达的意思是一样的。1.同义句在平常中多用于同义句转换，就是需要把一个句子用另外一种结构表达出来，但是要保证句意不能改变。2.英语中的同义句就是把句子中的一个或一些单词可以用别的意思相同的单词或句子代替，单词或句子用法不同也就导致句子结构不同。3.同义句转换的解题技巧：应弄清楚所给句子的内容和句式结构，试题填空部分与原句的对应关系，表达形式。根据所给空位，确定同义的句式和恰当的词语。对特殊结构的句型和习惯表达要仔细斟酌。同义句(synonymicsentences)亦称“同义异构句”。句法结构不同而命题意义相同的一组句子。如“老张批评了小王”、“老张把小王批评了”、“小王被老张批评了”，这三个句子就是同义句。同义句之间可以互相转换而命题意义不变；如果转换前后命题意义不同，则不是同义句。同义句在语义学中也称释义句，运用句法释义的手段可以来确立某些类型形式结构之间的转换关系。

stationn. 车站；驻地；地位；身分vt. 配置；安置；驻扎

流式细胞仪当然可以检测细胞内蛋白，这一点，并不是很难，当然要比检测细胞表面抗原复杂些，好在都已经有现成的方法，不成问题。不过流式的应用原理主要还是抗原和抗体反应和western、免疫组化没有本质差别，但是由于免疫组化和western都有酶催化底物的放大体系，因此， 流式不适合检测低表达的蛋白，低表达的还是采用western（尤其是化学发光底物更佳）和免疫组化更好。当然，流式最大的一个特点就是，可以定量（百分 比），如果是高表达的用流式细胞仪非常有优势。 流式检测时要求有抗体，可以是直接标记或简介标记的荧光抗体。细胞需要预先固定处理，常用的是多聚甲醛、TritoonX－100、75％乙醇等，具体步骤可参考相关文献，都有详细说明。………………详细资料请参考：on http://product.bio1000.com/100228/

歌曲名:Rosen歌手:Westernhagen专辑:In Den WahnsinnANNA inspi"NANA (BLACK STONES) - rose作词：ANNA作曲：宫崎あゆみ编曲：宫崎あゆみWhen I was darkness at that time 当陷于阴暗的那时震える唇 颤抖的嘴唇部屋の片隅でI cry 蜷在屋子一角，哭了起来もがけばもがくほど突き刺さるこの伤 越是拼命挣扎，越是将伤口撕裂破られた约束 hurt me 那被打碎的约定，伤害了我Nobody can save me 没人可以拯救我神様ひとつだけ 神灵只有一位止めて裂くような my love 能止住开裂的 是我的爱I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰舞い散る 悲しみ your song 凌乱舞步的悲伤，是你的歌居场所 ない 孤独なmy life 居无定所的孤独，是我的生活I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰Oh baby, help me from frozen pain 亲爱的，请将我从这冻结的伤痛中拯救with your smile, your eyes, 用你的微笑，你的眼眸and sing me, just for me 你的歌，只为了我I wanna need your love... 我需要你的爱I"m a broken rose 我是朵残破的玫瑰I wanna need your love... 我需要你的爱When you are with me at that time 当你在身边的那时贵方 の影を追いかけて 追逐着你的影子裸足（はだし）でかけ泣けてstop me 赤着脚狂奔 请停下我闭ざせば闭ざすほどもつれてくこの爱 越是闭上眼，越是被爱纠缠やるやけに优しく kiss me 轻缓温柔的，请亲吻我Nobody can save me 没人可以拯救我冻える蔷薇のように 像封冻的玫瑰优しく眠りたいmy tears 想要温柔的睡去 留下眼泪I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰彼 落ちる 悲しみ my soul 凋零坠落的悲伤，是我的灵魂离れてく孤独なlittle girl 因离别而孤独的，小女孩I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰Oh baby, help me from frozen pain 亲爱的，请将我从这冻结的伤痛中拯救with your smile, your eyes, 用你的微笑，你的眼眸and sing me, just for me 你的歌，只为了我I wanna need your love... 我需要你的爱I"m a broken rose 我是朵残破的玫瑰I wanna need your love... 我需要你的爱I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰舞い散る 悲しみ your song 凌乱舞步的悲伤，是你的歌居场所 ない 孤独なmy life 居无定所的孤独，是我的生活I need your love. I"m a broken rose. 我需要你的爱，我是一朵残破的玫瑰Oh baby, help me from frozen pain 亲爱的，请将我从这冻结的伤痛中拯救with your smile, your eyes, 用你的微笑，你的眼眸and sing me, just for me 你的歌，只为了我I wanna need your love... 我需要你的爱I was a broken rose 我是朵残破的玫瑰I wanna need your love... 我需要你的爱おわりhttp://music.baidu.com/song/936141

Develop Western Regions西部大开发 The large scale development of West China is of a profound significance. First, it will open a broad development space for central and eastern regions. Besides, it"s critical to China"s overall economic rise. What"s more, it"s the only effective way to decrease the imbalance of economic development in the east and the west so as to revitalize our nation.西部大开发是中国的深远意义。首先，它将打开一个广阔的发展空间，中部和东部地区。此外，至关重要的是，中国的整体经济增长。更重要的是，它是唯一有效的方法减少经济发展的不平衡，在东部和西部以民族振兴。 There are various reasons why the landlocked west has lagged behind in its economic development. The chief reason, I think, is that the opening up policies enables the east to lure more foreign capital and make a fantastic spurt in its development. As a result, it leaves the west far behind in its economic growth. In addition, the west"s unique unfavorable geographic positions greatly limit its development. Therefore, the gap between the east and the west have been widened since China‘s reform. In fact, many regions in the west still remain comparatively underdeveloped. To illustrate, most of the 30 million poor populations, who still have subsistence problems, live there. Moreover, currently the per capital GDP in West China is only about 60%of national average. And the vast West China makes less contribution to the nation"s development. If left un changed, the underdevelopment in the west will greatly affect overall prosperity and even social stability.有各种原因，内陆西部落后的经济发展。最主要的原因，我认为，是的开放政策使东吸引更多的外资和做一个迅猛发展。因此，它离开西远远落后于经济增长。此外，西部极为不利的地理位置限制了其自身的发展。因此，之间的差距，东方和西方都拓宽了中国自改革。事实上，许多地区仍然保持相对欠发达的西部。例如，大多数30000000贫困人口，谁仍然有生存问题，住在那里。此外，目前人均国民生产总值在中国只有60%的全国平均水平。和西部广阔中国贡献少的国家的发展。如果联合国不发达的西方化，将极大地影响共同富裕的目标甚至社会稳定。 However, the western region development is a long-term systematic project. It calls for tremendous efforts of several generations. To sum up, our chief goal is to build West China with economic prosperity, social progress, political stability and beautiful landscape. Finally, I believe the west will catch up with the east in economic development in the near future.然而，西部开发是一项长期的系统工程。它需要几代人的共同努力。总之，我们的主要目标是建立中国西部经济繁荣，社会进步，政治稳定和美丽的风景。最后，我相信西方将赶超东部经济发展在不久的将来。望采纳！！！

Pet Shop Boys---GO WEST 试听地址：http://idisk.mac.com/lucien0407/Public/mp3/Go_West_PetShopBoys.mp3

24小时。西联汇款是国际汇款公司（WesternUnion）的简称，是世界上领先的特快汇款公司，迄今已有150年的历史，在此公司撤回后需要24小时到账。它拥有全球最大最先进的电子汇兑金融网络，代理网点遍布全球近200个国家和地区。

是。WestUnion美国一家从事向美国的移民提供汇款、取款等现金服务的金融公司，是国际信用卡，美国是一个信用卡遍地开花的国家，现金业务被认为是昨日黄花。

西部联盟

Journey to the West" 的中文意思是 "西游记"。这是中国古代一部非常著名的小说，讲述了孙悟空、猪八戒、沙僧三人带着唐僧西天取经的故事。小说深入浅出地讲述了西天取经过程中的各种冒险经历、传说故事和道德寓意。

GoodRide好运牌、KUMHO韩国锦湖、GT佳通WestLake没有中文名称，因为这都是销往其他国家的一个牌子，在国内不销售。这个胎的质量上是有缺陷的是杭州中策橡胶所制造的轮胎。GoodRide好运牌是杭州中策橡胶所制造的轮胎，是朝阳轮胎系列产品，而KUMHO韩国锦湖轮胎,中国工厂在南京，GT在国内有好几个分厂，GT系列有佳安、路的金、登特路、兰威、长城等等。扩展资料：在选购公路自行车轮胎时大多会从滚动阻力、抓地力（又分干抓、湿抓）、操控性、使用寿命等方面来看，消费者也可以注意轮胎的帘纱密度，通常用TPI来表示。这个TPI是每英寸丝线数，帘纱越密轮胎的质量就越好，也可以进一步降低重量和滚阻，所以像Michelin的一级胎PowerCompetition，就强调用3层180TPI高密度帘纱层。除此之外滚阻只是车胎的一项性能表现，在竞赛中为求快会希望滚阻越低越好，但在不同的情境使用下，滚阻低却并不一定对骑乘是正向帮助。

WestLake 杭州中策橡胶所制造的轮胎 西湖牌GoodRide 好骑牌KUMHO,GT 韩国锦湖

Westlife: Walk AwayAlbum:World Of Our OwnTitle:Walk AwayHere we are Just a little older Time goes by But did I ever tell you why I want you so much It came to me When you were not around You"ll always be The one to understand that"s why I can"t miss your touch All my life I waited for someone And all this time You were the one So If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you I"ve been a fool I didn"t see behind The friend in you And baby don"t you ask me why It just couldn"t be love Cause from the start You would be the one To cure my heart But I just didn"t look this way And now I can"t get enough All my life I waited for someone And all this time You were the one, so If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you And with a kiss you turned my world around The greatest rush of all was to be found Oh yeah You changed my whole life Coloured up the dark skies And this I promise you If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you

是Shadows

http://www.fnetravel.com/hotelprofile/index.aspx?hc=4051

https://pan.baidu.com/s/1K-7fCMQm6VYrbmvZxmKtaA 提取码:vleqhttps://pan.baidu.com/s/1bLERaueF0WdFabbDbh45gw 提取码: 6914宫崎骏所有动画作品1968年《太阳王子霍尔斯的大冒险》、1981年《鲁邦三世：卡里奥斯特之城》、1984年《未来少年柯南特别版剧场版》、《风之谷》、1986年《天空之城》、1988年《龙猫》、1989年《魔女宅急便》、《红发少女安妮 》。1991年《岁月的童话》、1992年《红猪》、1994年《百变狸猫》、1997年《幽灵公主》、2002年《猫的报恩》、2004年《哈尔的移动城堡》、《胶卷咕噜咕噜转》2010年《借东西的小人阿莉埃蒂》、2011年《虞美人盛开的山坡》、2013年《起风了》、《空想的飞行机械们》、《酵母君与鸡蛋公主》、《竹取物语》、《毛毛虫菠萝》、《胡桃夹子与老鼠王》。编剧动画片：1972年《熊猫家族》、2001年《千与千寻》、《风之谷》、 《侧耳倾听》 、《地海战记》、《Yadosagashi》、《种下星星的日子》《小狗克罗历险记》、《悬崖上的金鱼公主》、《借东西的小人阿莉埃蒂》、《阿尔卑斯山的少女》。宫崎峻的动画片是能够和迪士尼、梦工厂共分天下的一支重要的东方力量。宫崎峻的每部作品，题材虽然不同，但却将梦想、环保、人生、生存、和平主义、女权运动这些令人反思的讯息，融合其中。他这份执着，不单令全球人产生共鸣，更受到全世界所重视，连美国动画王国迪士尼，都要斟介宫崎骏的动画电影发行版权（亚洲地区除外）。宫崎峻在全球动画界具有无可替代的地位，迪士尼称其为泰戈尔曾说过：世界上最遥远的距离不是生与死的距离，而是我站在你面前，你却不知道我爱你。这就是我与你的距离，虽然就站在你面前，但这触手可及的距离、有时也遥不可及。

simon 西蒙 这是个英文中比较普遍的名字，希伯来文，意为听者。SIMON被视作强壮魁梧的男子，若非有着安静样的个性就是聪明而富创造力。 这个名字还蛮好的west好像是个姓氏，就不必要了吧。

后者是西联汇款我做过。就是提供给客户正确的姓名和卡号。千万要注意姓名，我上次和客户说的很清楚姓和名了，结果他还是把姓和名搞错了。导致最后我们第一次没有成功，后面又重新办理了一次。

西联汇款嘛？就在沙湾路和北二环交接处的邮政营业厅就有西联汇款估计成都大的邮政营业厅都有把

WESTERN UNION境外汇款程序：1.申请书要用英文准确填写。2.汇款货币及金额（阿拉伯数字）。3.收款人姓名及地址。4.收款人在开户银行的账号。5.收款人开户银行名称、SWIFT代码（SWIFT CODE）或地址。WESTERN UNION：西联汇款是国际汇款公司(Western Union)的简称，是世界上领先的特快汇款公司，迄今已有150年的历史，它拥有全球最大最先进的电子汇兑金融网络，代理网点遍布全球近200个国家和地区。西联公司是美国财富五百强之一的第一数据公司(FDC)的子公司。

中国有资金限制吗？

中国光大银行、中国邮政储蓄银行、中国建设银行、浙江稠州商业银行、吉林银行、哈尔滨银行、福建海峡银行、烟台银行、龙江银行、温州银行、徽商银行、浦发银行。西联汇款是国际汇款公司（WesternUnion）的简称，是世界上领先的特快汇款公司，迄今已有150年的历史，它拥有全球最大最先进的电子汇兑金融网络，代理网点遍布全球近200个国家和地区，数量达135000多个，且不断增加中。

WU是和农业银行联合的，所以如果国内有亲人的话可以去农行取出之后再汇入你的账号。routing number是接收银行的银行代码，可以打电话去银行查询。

你有机会通过西联银行支付？

手续费肯定是要收的，但是西联汇款的手续费用是汇款时一次性收费。具体怎么收费你可以去你汇款的银行网点查询关于汇款手续费问题，当然也可以打电话问西联汇款的客服。

可以的。1、银行汇款（存款）的流程：在确定收款人提供的存折帐号、户名无误的话，拿现金到银行直接存入收款人帐号就可以了；2、建议你在银行开一个帐户，然后填一份电汇单，填写清楚对方的收款帐号、户名、开户行名称，从帐户上汇款过去收款人；3、如果嫌开户麻烦的话，就可以直接用现金汇款，填一份电汇单进行汇款，不过一定要填写清楚对方的收款帐号、户名、开户行名称；4、开通网上银行，自己到网上击活，就可以网上支付、转账。

　　直接去国内的六大银行，都有西联的办理点，办一个账号就OK了，相当于办张银行卡！其他操作模式按照规矩来

1） 到相应的西联代理点，填写汇款表单： 填写汇款表单，提供由政府发行的身份证明。 2） 支付汇款手续费： 将要汇出的款额连同必要的服务费用一起交给代理点。 3） 签名并接收收据： 确认收据上的所有信息均无误之后，需要签署一张收据。收据所打印的内容之一是汇款监控号码(MTCN)。可使用MTCN联机（在网上）跟踪汇款的状态 。 4） 通知收款人（国内的商户）： 国外的客户将支付的信息告诉国内商家， 如汇款人姓名、汇款金额、汇款监控号码（MTCN）和汇款国家/地区。 5） 款项的跟踪。： 国外的客户可以到西联的主页上，进入跟踪的页面。然后输入汇款人姓名和汇款监控码(MTCN）来跟踪汇款的状态。

西联和TT汇款性质差不多的，没有任何约束！

Western Union 是一家美国公司，全球国家之间汇款都可以即时到账的。收款人凭身份证及一组10位数密码可以在合作金融机关提领，手续费超低。在中国也有合作的金融机关，如邮局、建行、农行、光大银行及许多地方性的银行；但是妙就妙在，笔者曾经为了一个美国汇来的区区1000元美金，跑遍了它网页上的合作金融机关、再打电话到Western Union在中国上海的总部，由服务人员提供的北京网点照着去，也被告知“尚未有如此服务”至今尚未解决。为何国际通行的服务到了此地就变了味道，耐人寻思。

西联汇款的英文就是WESTERNUNION！就好比中国的英文是china一样。如果办理业务可以直接去西联就可以了，这个汇款主要针对往国外汇款的！

欧盟全球展览--------------------精锐老师很高兴为服务

这是western union.全球最快的汇款方式,只是手续费比较高.中文名是"西联汇款”,国内邮局和农行可以办理.我在巴基斯坦工作,偶尔回国时老板会从巴基斯坦给我用西联汇款,我可以很快从任何一个城市取.他那边需要填写你的名字和地址,然后还有一个监控号码(control number),相当于密码,他得到后告诉你.取钱时带上身份证,有时还得要身份证复印件.可以取人民币或美元.但是有几个问题得注意下:1. 如果数量比较大,西联汇款手续费太高,最好选银行转帐方式,像常用的工商银行,这边用渣打转.2. 名字用拼音写,但是注意顺序.有一次别人把我的名和姓写反了,邮局不给取,后来打电话给巴基斯坦的汇款人修改以后才取到.3. 地址不用很详细,写明城市就行了.

没有关系的,你只要给他一个地址,到时候客户给钱的时候,客户给你一个码,你就可以去领取啦.

WESTERN UNION汇款程序很简单，和人民币一样到银行柜台填电汇申请书就行。附注：申请书要用英文准确填写。汇款货币及金额（阿拉伯数字）。收款人姓名及地址。收款人在开户银行的账号。收款人开户银行名称、SWIFT代码（SWIFT CODE）或地址。WESTERN UNION是一种近乎实时到帐的快速汇款方式。中国农业银行、中国光大银行、中国邮政储蓄银行 、中国建设银行、等多家银行西联公司签订了合作协议。当然，由于中国现在是外汇管制国家，不允许不受限制的汇出汇款，故一般以2000美元为限。如果是学费，境外援助等有证明的，可以要求西联公司给与授权。

western union西部联盟；西联汇款；西联国际汇款公司；西联公司例句1.How much money can I send by Western Union?通过西联汇款方式我可以寄多少钱？2.We also accept payment using Western Union or wire transfer.我们也接受使用西联汇款或电汇付款。3.First, I must tell you we only accept western union!首先我必须要告诉你，我们只接受西联！4.You are to make the payment Via Western Union Transfer or Money Gram to this office.您将通过西部联盟调动或金钱克付付款给这个办公室。5.well babe i just got back from the western union and sent you a thousand dollars.以及贝贝我刚回来的西部联盟，并送你一千元。

是“西部联盟电报公司”的意思

随意 泡一会用stripping buffer洗就行了

如果是PVDF膜就没事，你扫描了么？有时候膜干了条带更清楚。如果是硝酸纤维素膜就有点麻烦，因为干了一碰就碎，不知道你是不是用拍照的。

请问你再生后封闭了吗？如果没封闭直接孵育其他抗体很有可能出现高背景。这张膜恐怕不能再用了。

Kanye West - Stronger is this the window to my heart i just want you to be free there ain"t no freedom where we are ain"t no wishes in these stars ain"t no reason to believe but don"t worry baby don"t you worry maybe this is what we need a little bruising, a little bleeding some space that we can"t breathe in some silence in between so cry for me baby, and i"ll cry for you and we"ll both break down and we"ll both break through we"ll find our way and we"ll face the truth we both will be stronger and we"ll lie down in our loneliness and wake up with our sad regrets even though we don"t know it yet we both will be stronger we both will be stronger i can"t believe you"re really gone now but i know it"s for the best and i know that we weren"t right but i still reach for you each night and man that hurts like hell so baby this is the window to my heart i just want us to be free baby i"m sorry for the way things are goodbye is always hard and we both will be stronger 氮氮，现在次，不杀我 只能让我强 我需要你快点现在 导致我不能再等待了 我知道了现在是正确的 导致我无法多wronger 男子我已经waitin"现在整夜 这就是我多久就被亚 我需要你现在 我需要你现在 让我们失去了今晚 你可能是我今晚的黑色凯特莫斯 玩书记，我是老板今晚 你不给自动对焦**亩他们说什么吗？ 真棒，基督教和和迪奥 该死的，他们不会让"时间再这样 我要问，导致我不知道 没有任何人做出实实在在的狗屎了吗？ 弓的伟大的存在 现在你的事业已遗弃我们 你应该得到尊重，我的迟到 说我什至出现这种假狗屎 所以要大胆向前走坚果发疯狗屎 特别是对我最好的站在我的巴佩狗屎 喜欢你的行为不知道谁提出这 新传家一样，需要半年，并借此，憎恨 氮氮，现在次，不杀我 只能让我强 我需要你快点现在 导致我不能再等待了 我知道了现在是正确的 导致我无法多wronger 男子我已经waitin"现在整夜 这就是我多久就被亚 我需要你现在 我需要你现在 我不知道你有一个人与否， 如果您做了计划或不 神放在计划或不是我 我trippin"这种饮料让我萨延"了很多 但我知道上帝把我面前的你 到底怎么能在你面前的我？ 有1000你的，还有只有我一个 我trippin"，我陷入了暂时的权利呢？ 这是路易威登角钱夜 所以我们坤"尽最大努力，像根 听说他们会做任何事情的克朗代克 同时我会做一个金发碧眼，堤防什么 她会做任何事情的风头 我们会做任何事情时，时间的权利 唉，宝宝，你马金"它（难，更好，更快，更强） 氮氮，现在次，不杀我 只能让我强 我需要你快点现在 导致我不能再等待了 我知道了现在是正确的 导致我无法多wronger 男子我已经waitin"现在整夜 这就是我多久就被亚 我需要你现在 我需要你现在 你知道我多长时间在亚了？ 因为王子是在阿波罗尼亚 因为辛普森曾isotoners 不要像我从来没有告诉亚 孩子，你使（困难，更好，更快，更强） 氮氮，现在次，不杀我 只能让我强 我需要你快点现在 导致我不能再等待了 我知道了现在是正确的 原因i无法取得很大wronger 男子我已经waitin"现在整夜 这就是我多久就被亚 我需要你现在 你知道我多长时间在亚了？ 因为王子是在阿波罗尼亚 因为辛普森曾isotoners 不要像我从来没有告诉亚 从来没有告诉亚

歌曲名:Stronger歌手:Kanye West专辑:Now That"s What I Call Music! 10Work it, make it, do it,Makes us harder, better, faster, STRONGER!Now that don"t kill mecan only make me strongerI need you to hurry up nowcause I can"t wait much longerI know I got to be right nowcause I cant get much wrongerman i"ve been waitin" all night nowthat"s how long i"ve been on yaI need you right nowI need you right nowlets get lost tonightyou could be my black kate moss tonightplay secretary i"m the boss tonightand you don"t give a f*ck what they all say right?awesome, the christian and and kristen diordamn they don"t make "em like this anymoreI ask, cause i"m not suredo anybody make real shit anymore?bow in the presence of greatnesscause right now thou has forsaken usyou should be honored by my latenessthat I would even show up to this fake sh*tso go ahead go nuts go ape sh*tspecially on my best stand on my bape sh*tact like you can"t tell who made thisnew gospel homey take six, and take this, hatersNow that don"t kill mecan only make me strongerI need you to hurry up nowcause I can"t wait much longerI know I got to be right nowcause I cant get much wrongerman i"ve been waitin" all night nowthat"s how long i"ve been on yaI need you right nowI need you right nowme likeyI don"t know if you got a man or not,if you made plans or notgod put me in the plans or noti"m trippin" this drink got me sayin" a lotbut I know that god put you in front of meso how the hell could you front on methere"s a thousand you"s there"s only one of mei"m trippin" i"m caught up in the moment right?this is louis vuitton dime nightso we gon" do everything that kan likeheard they"d do anything for a klondikewell i"d do anything for a blonde-dikeand she"ll do anything for the limelightand we"ll do anything when the time"s rightugh, baby you"re makin" it (harder, better, faster, stronger)Now that don"t kill mecan only make me strongerI need you to hurry up nowcause I can"t wait much longerI know I got to be right nowcause I cant get much wrongerman i"ve been waitin all night nowthat"s how long i"ve been on yaI need you right nowI need you right nowyou know how long i"ve been on ya?since prince was on apolloniasince OJ had isotonersdon"t act like I never told yababy you"re making it (harder, better, faster, stronger)Now that don"t kill mecan only make me strongerI need you to hurry up nowcause I can"t wait much longerI know I got to be right nowcause I cant get much wrongerman i"ve been waitin all night nowthat"s how long i"ve been on yaI need you right nowyou know how long i"ve been on ya?since prince was on apolloniasince OJ had isotonersdon"t act like I never told yaYou know how long I"ve been on ya?Since prince was on ApolloniaSince OJ had IsotonersDon"t act like I never told yanever told yanever overhttp://music.baidu.com/song/59408251

三月份Kendrick Lamar推出To Pimp a Butterfly，我心中便暗暗叫苦：面对这般份量的作品，一般的独立乐队／歌手哪是对手？今年还有Frank Ocean、Miguel、Kanye West的新专辑，随便一张都有年度榜单霸主的潜力——法海将于七月份发行的专辑已成为本年度最受期待专辑——难道今年独立音乐要沦为全年陪（黑人音乐）跑的角色吗？——好在三四月份还有一帮民谣歌手／乐队扎堆发片，我们迎来了Laura Marling、Villagers、Courtney Barnett、Tobias Jesso Jr.，以及时隔五年推出新专辑的Sufjan Stevens（人送昵称“舒肤佳”）。从媒体评分看，Sufjan Stevens的Carrie Lowell有可能成为今年综评最高的民谣专辑，甚至有潜力冲击年度榜单十强。看歌词即知，Carrie Lowell是一张非常私人的专辑。Carrie Lowell是他母亲和继父的名字。Carrie不是一个合格的母亲，她有精神分裂症和抑郁症，有瘾和酒瘾，曾反复遗弃Sufjan Stevens（在Should Have Known Better中他唱道：“When I was three, three maybe four，She left us at that video store”），2012年Carrie因胃癌去世。Sufjan Stevens对她抱有复杂感情，在不少歌词中描述了他试图靠近母亲而不得的状态，以及母亲去世对他的影响（“Do I care if I survive this? Bury the dead where they"re found”），因此在歌词中反复用到“鲜血”、“死亡”和“阴影”的意象。相反，继父Lowell与Sufjan Stevens关系更亲密，在Sufjan Stevens五岁到八岁期间，每年Lowell都会接他到俄勒冈洲的家中玩，教他游泳（“The man who taught me to swim, he couldn"t quite say my first name”），这是Sufjan Stevens童年中最快乐的回忆，歌唱这段往事的Eugene也是专辑中最轻快的歌曲。了解Sufjan Stevens的身世再看歌词，会帮助你更好了解／接近作品。有趣的是John My Beloved的歌词，似乎印证了坊间对舒肤佳性取向的猜测（咳咳咳.从形式上看，这次Sufjan Stevens返璞归真的选择以acoustic guitar为核心，辅以键盘和氛围音乐的方式。我刚开始听，认为“啊，acoustic guitar，无聊无聊无聊！”后来发现太偏颇。你可以将其类比Elliott Smith、Nick Drake甚至（弱化版）Bon Iver。配器精巧的组合起来，空旷的氛围乐暗示了Sufjan Stevens低沉的心境，清脆的吉他则代表一抹亮色。专辑听感舒适，可以无压力循环一整天，配合歌词效果更佳。我最近比较迷Sharon Van Etten，就形式上看，Carrie Lowell可能略为平淡，但内容来说，以刀般剖析自我（连masturbated都写出来了），这番勇气非常人所及。