动物实验

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开放系统的动物实验室适用于饲养什么实验动物

开放系统的动物实验室适用于饲养普通级实验动物。实验动物房设施分类如下:1、开放系统,适用于饲养普通级实验动物。2、亚屏障系统,适用于饲养清洁级实验动物。3、屏障系统,适用于饲养无特定病原体级动物。4、隔离系统,适用于饲养SPF级及无菌级实验动物。实验动物房设备主要包括:1、空调和空气净化设备。动物房的空调和空气净化系统设计宜采用集中式,机房一般布置于建筑的底层和顶层。动物房内的温、湿度的控制通过空调机组实现。普通动物房对空气只作一般的除尘过滤,但屏障系统和隔离系统对空气净化要求极为严格。2、给水和排水设备。用水不能低于自来水规定的清洁标准。屏障区内的用水,应全部进行消毒过滤。饮水和用水分别采用不同的消毒药液进行消毒。隔离系统内的动物饮用水,应设置独立的饮用水装置。动物室的废弃水,要有统一的污水系统,经处理后排除。

中国动物实验学报是几类杂志,急!!

是中国实验动物学报吧,中国实验动物学会的杂志。是国家级杂志,中文核心期刊。

心理学代表人与动物实验的总结

人或者是动物行为带有一定的思想

生化与分子生物学的研究生一定会做动物实验吗?经常做吗?

这个要看导师做的课题了,有一些导师做植物bai,有些做微生物du,有些做细胞,有些做药物,有些做食品,从这些分类你zhi可以大致看出来做的侧dao重点了吧?基础医学院的分子生物学和偏向做神经、药物和内疾病研究的分子生物学方向应该都是需容要经常做动物实验的。

分子生物学实验 是不是都必须要涉及到动物实验啊?

植物学的就不用,当然要想搞研究建议分子生物学方面 比较有发展 也不用动物实验

动物实验室怎么设计?

一.实验动物分级及其标准根据实验动物微生物控制标准,可将实验动物分为四级:一级 普通动物(CV),系指微生物不受特殊控制的一般动物。要求排除人兽共患病的病原体和积少数的实验动物烈性传染病的病原体。为防止传染病,在实验动物饲养和繁殖时,要采取一定的措施,应保证其用于测试的结果具有反应的重现性(即无论不同的操作人员,在不同的时间,用同一品系的动物按规定的实验规程所做的实验,都能获得几乎相同的结果)。二级 清洁动物(CL),要求排除人兽共患病及动物主要传染病的病原体。三级 无特殊病原体动物(SPF),要求到二级外,还要排除一些规定的病原体。其除菌与灭菌的方法,可使用高效空气过滤器除菌法、紫外线灭菌法、三甘醇蒸气喷雾法及氯化锂水溶液喷雾法。四级 无菌动物(GF)或悉生动物(GN)。无菌动物要求不带有任何用现有方法可检出的微生物。悉生动物要求在无菌动物体上植入一种或数种已知的微生物。在病理学检查上,四类实验动物也有不同的病理检查标准。一级 外观健康,主要器官不应有病灶。二级 除一级指标外,显微镜检查无二级微生物病原的病变。三级 无特殊病原体动物。无二、三级微生物病原的病变。四级 不含二、三级微生物病原的病变,脾、淋巴结是无菌动物组织学结构。综合上述,对不同级别的实验动物在动物房设计上和管理上则有不同的要求。无菌、已知菌以及无特殊病原体动物都需要在无菌或尽可能无菌的环境里饲养,这种环境,目前国际上通用称为屏障环境,即用一道屏障把动物与周围污染的环境隔开,就如胎鼠在母鼠子宫内一样。这种环境从控制微生物的角度分为隔离系统、屏障系统、半屏障系统、开放系统和层流架系统等五大类。A 隔离系统 是在带有操作手套的容器中饲养动物的系统,用于饲养无菌动物和栖生动物。内部保持按微生物要求的100级的洁净度,但其设置的房间及操作人员不必按无菌室考虑。B 屏障系统 把10000~100000级左右的无菌洁净室作为饲养室,主要用于无特殊病原体动物的长期饲养和繁殖。入室施行严格管理,如淋浴、换贴身衣服等。C 半屏障系统 放宽对屏障系统中人及物出入房间时的管理,平面组成大致与屏障系统相同。D 层流架系统 笼具放在洁净的水平层流空气中。常用于小规模饲养,但在一般房间进行饲养、操作和处理时有被污染的危险性。可用于半屏障的补充。E 开放系统 是对人、物、空气等进出房间均不施行消除污染的系统,但通常要进行某种程度的清洁管理。下列出了各种系统对 实验动物环境指标的要求:◆ 项目指标 开放系统(CV) 简易屏障系统(CL) 屏障系统(SPF) 隔离系统(GF,GN)◆ 温度/℃ 18~29 18~29 18~29 18~29 ◆ 日温差/℃ 8 3 3 3 ◆ 相对湿度/% 40~70 40~70 40~70 40~70 ◆ 换气次数/(次/h) 10~15 10~15 10~15 ◆ 气流速度/(m/s) 0.18 0.18 0.18 ◆ 压差/Pa 20~50 20~50 20~50 ◆ 洁净度/级 10万 1万 100 ◆ 菌落数/[个/(皿?h)] 12.2 2.45 0.49 ◆ 氨的质量浓度/(mg/m3) 14 14 14 14 ◆ 噪声/dB ≤60 ≤60 ≤60 ≤60 ◆ 照度/lx 150~300 150~300 150~300 150~300 达到动物房环境指标的净化措施有:A.空气净化系统 因为动物房是实验动物较长时间活动的场所,室内不可能在饲育条件下经常用薰蒸冲洗的方式灭菌,一般只能用药力较弱的消毒液檫洗,在这种情况下为了避免微生物侵染,保证空气净化则是首要条件;B.全新风 防止交*污染和除臭是两个难题,为此,净化空调系统大部分必须采用全新风方式,但由于净化系统换气次数大,又是全新风,所以能耗问题突出;C.除臭 这不仅是对外部环境和工作人员所必须,而且也是实验动物本身所必须。因为室内除外界微生物污染外,动物本身排泄物和微生物二次转化生成的氨、硫化氢、醇等气体,也是重要的污染物质,这些气体臭味愈浓烈,说明动物本身的洁净度愈低。目前还无臭气允许值的定量资料和测定方法,但对于氨浓度应进行控制。除臭一般有增加新风和活性炭吸附两种方法。二.动物房一般环境因子控制范围(1) 温度 目标值为21~27℃。药典规定为17~28℃。可因动物品种而不同。(2) 湿度 45%~55%。卫生部规定为40%~60%。(3) 气流速度 10~25cm/s。避免直接吹风。(4) 换气次数 6~15次/h。新风为2/3。(5) 气压 洁净区正压,感染区负压。(6) 环境洁净级别 一般动物饲养室大于100000级。(7) 照明 150~300lx(人工照明)。(8) 噪音 40~50dB(无动物时),有动物时为60 dB,卫生部规定小于70 dB。(9) 臭气 氨20ppm。三.动物房设计的基本要求(1) 选址 僻静、卫生(2) 布置 分为准备区、饲养区、实验区。要求人流、物流、动物流分开(单向流程);要求净化、灭菌、防虫。(3) 建筑 要求有洁净走廊,饲养室,污染走廊以及其他各室。(4) 空调系统 有可控制的温度和湿度、气流速度和分布,有一定的换气量和气压。(5) 照明 无窗动物房使用荧光灯,有窗动物房可安装玻璃窗,以滤去紫外线。要求12h亮,12h暗。(6) 供水 有饮用水和精制水。四.动物房的设置根据不同种类和不同级别实验动物的需要,建立相应设施的动物房、活动场所和相应的辅助用房。场址应选在能保持安静、清洁、无不良外界影响的地方。动物房必须光线充足、通风良好、地面整洁,不积水;顶棚、墙壁要易于清洁、消毒;外墙、屋顶、顶棚、门窗及通外面的管道等必须杜绝外界动物、蚊蝇及其它虫害钻入。室内温度控制在18~29℃,相对湿度40%~70%,噪音60 dB以内。笼内动物密度不能太大,要有送风、排风、降温及保暖设备。必须有专用的排污、排水设施,防止病原扩散。各种笼具要定期清洗消毒,垫料须经高温高压或药物消毒并及时更换,使室内氨浓度在15 mg/m3以下。

山东生命科学城做无菌动物实验室该怎么设计布局?

你可以借鉴一下上海动物房工程EPC优秀代表CEIDI西递的经验,这家公司在江浙沪区域口碑蛮高的,不少工程都成为了行业标杆:1、无菌动物实验室的人员、动物和物品流向均须严格按照单向流动的原则布局(1)人员流向为外环境→更衣室→缓冲区→洁净走道→实验室→洁净走道→缓冲区→外环境。(2)动物流向为购买实验动物→检疫室→饲养室→洁净走道→缓冲区→解剖室。(3)物品流向为清洗间→高压灭菌/传递窗→洁物暂存室→实验室→缓冲区→外环境。2、动物房、动物实验室及洁净走廊都要采用全新风直流式系统,洁净区各房间换气次数均为20次/h。3、实验动物会产生许多污染物,考虑到动物实验的排风味道较大,在高空排放(排风距风机所在屋面 2.5 m 以上)的同时设置活性炭除味装置,活性炭过滤吸收器则设置在负压段,这种方法可避免臭味通过排风管泄漏。希望给个大大的赞

动物实验室的建设流程是怎样的?

医学史上许多重大成就的发现都依赖于动物实验,不仅如此,不少生理学知识的获得以及外科手术技能的提高也都来源于动物实验。因此,动物实验是科学研究过程中必不可少的条件资源和重要手段。研究人员在实验动物时一定要在安全可靠无污染的动物实验室里面进行。那么,科研公司要是想搭建动物实验室,建设流程是怎样的呢?我们可以参考一下专业的实验室工程EPC总包CEIDI西递的做法。 CEIDI西递建设动物实验室时流程一般如下: 动物实验室布局的设计→水电预留位置的设计→通排风系统的设计→气路管道系统的设计→环保的设计→产品个性化设计→安全设施的设计 为了让各科研公司少走点弯路,清楚了解如何从0到1做好动物实验室的建设,我们把“动物实验室布局的设计”这一部分重点来展开说说。 研究人员在实验动物时很容易会受到环境的干扰,动物在饲养过程中也容易产生异味,影响其他生产或生活用房,因此,在动物实验室总体布局的选址和建造时一定要注意以下几个方面: 1.最好独立建造动物房; 2.避开车流和人流密集处,避开厂区震动和噪声大的区域; 3.选择在全年主导风向下风处,且对厂区其他生产或生活设施影响最小的区域; 总的来说,就是要避开自然疫源地,远离可能产生交叉感染的动物,最好与生活区保持大于50m的距离。 另外,动物实验室从功能间设置上主要包括人员的更衣间、动物饲养间、实验间、动物检验间、洁净物品存储间、清洗间、解剖间、饲料和垫料的存储间等,各功能间设置需齐全、合理。还要严格区分清洁区域和污染区域,以保证人和动物的健康,确保实验的准确性。 实验动物是支撑生命科学基础研究的重要资源,因此,此类实验室的建设设计都必须要经过科学的论证。随着有关法规和涉及实验动物及设施的新国标的实施,动物实验的标准不断提高,实验设施和动物的管理也将进一步加强。各科研机构在建设设计动物实验室时一定要找像CEIDI西递这种专业提供实验室建设技术与服务的公司,尽可能减少现有问题的重复出现,才能做好自己的工作,乃至推动实验动物学科的发展。

小鼠动物实验设计方案怎么写?

小鼠动物实验设计方案通常包括以下几个部分:1、研究目的清楚地阐明实验的科学目的以及研究意义,并说明预期的研究结果和应用价值。2、研究背景简要说明相关研究领域已有的研究进展及存在的问题,说明该研究的学术意义和现实价值。3、材料与方法详细介绍小鼠的选取、饲养、处理方法、实验组设计、样本收集、数据分析等,包括实验流程、实验方法、仪器设备和试剂等。4、实验方案(1) 实验对象:说明实验选取的小鼠品系、年龄、性别,以及样本数目等。(2) 实验组设计:详细说明控制组和实验组的设置,如正常对照组、模型组、药物处理组等。(3) 实验方法:具体说明实验过程,如给药途径、药物剂量、处理时间、观察指标以及样本收集方法等。(4) 数据处理:描述实验数据的收集、分析和解释方法,包括统计学方法和图表的绘制等。5、预期结果预测实验结果,包括可能出现的偏差和误差,如何避免和纠正。6、预算和时间计划列出实验所需费用和预算,以及每个环节的时间计划。7、实验安全说明实验过程中需要注意的安全事项以及应急措施。8、参考文献列出参考文献,标明资料来源。小鼠动物实验设计方案应该包括上述内容,并在合适的情况下根据你的具体研究进行适当的调整和完善。

动物实验的原理是什么?

写作思路:说明研究动物的初衷和重要的原因,比如人类开展动物实验的初衷是为了了解和认识生命现象,怀疑和挑战宗教神学对人类思想的禁锢;人类研究动物的原因是在不能以人为实验对象的前提下,科学研究必须借助实验动物和动物实验,进行疾病机理和诊断治疗方法的研究,在安全、有效的情况下再推用到人类。正文:The original intention of animal experiment is to understand and understand the phenomenon of life, doubt and challenge the confinement of human thought by religious theology. This exploration and understanding has formed the basic life science theories of zoology, anatomy and physiology.人类开展动物实验的初衷是为了了解和认识生命现象,怀疑和挑战宗教神学对人类思想的禁锢,这种探索和认识,形成了动物学、解剖学、生理学等基础生命科学理论。When the understanding of normal anatomy and physiology of human and animals is gradually clear, the prevention and treatment of disease research began. Thanks to animal experiments, the theoretical system of modern medicine such as immunology, microbiology, infectious diseases and virology has been gradually formed.当对人体和动物正常解剖与生理的认识逐步清楚后,才开始疾病的预防和治疗研究。得益于动物实验,逐步形成了免疫学、微生物学、传染病学、病毒学等现代医学的理论体系。In the game between human and disease, cholera, smallpox, polio and other infectious diseases have taken a large number of innocent lives. Scientists have found that many human diseases occur in some animals as well.人类与疾病的博弈中,霍乱、天花、脊髓灰质炎等传染病的暴发夺走了大量无辜的生命。科学家发现很多人类的疾病,在某些动物身上也会同样发生。On the premise of not using human as experimental objects, scientific research must rely on experimental animals and animal experiments to study the disease mechanism, diagnosis and treatment methods, and then apply it to human beings under safe and effective conditions.在不能以人为实验对象的前提下,科学研究必须借助实验动物和动物实验,进行疾病机理和诊断治疗方法的研究,在安全、有效的情况下再推用到人类。

关于动物实验的英语作文~

ExtinctionExtinction is a natural process. Many species had ceased to exist before humans evolved. However, in the last 400 years, the number of animals and plants becoming extinct has reached crisis point. Human population levels have risen dramatically in the same time period and man"s predatory instincts combined with his ruthless consumption of natural resources are directly responsible for the situation. DodoThe Dodo is a classic example of how human behavior can cause irreparable damage to the earth"s biological diversity. The flightless Dodo was native to the Island of Mauritius in the Indian Ocean. It lived off fruit fallen from the island"s trees and lived unthreatened until humans arrived in 1505. The docile bird became a source of food for sailors and lacked the ability to protect itself from animals introduced to the island by humans such as pigs, monkeys and rats. The population of Dodos rapidly decreased and the last one was killed in 1681.Endangered AnimalsIn 2002 many animals remain threatened with extinction as a result of human activity. The World Wildlife Fund works tirelessly to raise awareness of the predicament facing these animals and find ways to protect them. By focusing on a number of high profile, "charismatic icons" such as the rhino, panda, whale and tiger, the WWF aims to communicate, "critically important environmental issues". The organization"s ultimate goal is to, "stop the degradation of the planet"s natural environment and to build a future in which humans live in harmony with nature".RhinosThe Rhino horn is a highly prized item for practitioners of Asian medicine. This has led to the animal being relentlessly hunted in its natural habitat. Once widespread in Africa and Eurasia, most rhinos now live in protected natural parks and reserves. Their numbers have rapidly decreased in the last 50 years, over half the remaining rhinos disappeared in the 1970s, and the animals remain under constant threat from poachers.The Giant PandaThe future of the WWF"s symbol is far from certain. As few as 1000 remain in the wild, living in small isolated groups. These groups have been cut off from each other as a result of deforestation and human expansion in to their natural habitat. The Chinese government has set up 33 panda reserves to protect these beautiful animals and made poaching them punishable with 20 years in prison. However, the panda"s distinct black and white patched coat fetches a high price on the black market and determined poachers still pose one of the most serious threats to the animals continued existence.WhalesThe International Whaling Commission meets every year. The agenda covers ways to ensure the survival of the species and the complex problems arising from countries such as Japan, wishing to hunt certain whales for "scientific" purposes. Despite the fact that one third the world"s oceans have been proclaimed whale sanctuaries, 7 out of 13 whale species remain endangered. The plight of the North Atlantic Right Whale is particularly serious. Hunted for their rich supply of oil, their numbers have dwindled to just 300. Collisions with ships, toxic pollution and becoming entangled in fishing nets are other major causes of whale deaths.TigersThe last 100 years has seen a 95% reduction in the numbers of remaining tigers to between 5000 and 7000 and The Bali, Javan, and Caspian tigers are already extinct. The South China tiger is precariously close to disappearing, with only 20 to 30 still alive. Like the Rhino horn, tiger bones and organs are sought after for traditional Chinese medicines. These items are traded illegally along with tiger skins.Take ActionThe WWF is actively involved in many areas of the world fighting to protect the natural habitats of endangered animals from further damage and curb the activities of poachers. They also work to influence governments and policy makers to introduce laws aimed at reducing the threat of pollution and deforestation. Our own individual efforts at home and in the workplace can also make a difference. By reducing waste and pollution, saving water, wood and energy, and reusing and recycling whenever possible, we can reduce the possibility of even more animals being lost, never to return.Your turnDo you think enough is being done to protect animals? If not, what else could be done? Send us your opinions.Far out into the ocean to the north and west of Britain are the cold wild islands of the Outer Hebrides of Scotland. They make a line of beautiful beaches 150 miles long. Further west is the small group of islands called Saint Kilda. They are cold and wild too, but without beaches. The islands are tall volcanic rocks hundreds of metres high. For thousands of years, people lived on these islands. In 1930 the last people, there were only 36 of them, had a meeting and decided to leave. The biggest island in the group is called Hirta. Sheep still live there without any people. When you arrive by boat, you see very tall black rocks all around. Some big rocks make their own small islands. This true story happened on the tallest of the rocks – “Stac An Armin” in 1840.In those days, people still lived on the islands of Saint Kilda. Their stone houses were all in one village by the ocean at the bottom of a tall dark hill. The houses only had one room – for people and sheep, which used to live with them in the winter and spring. Two men from the village, McDonald and McKinnon, were on the rock. It was their work to collect birds – some for food, some to make shoes or hats with. Some dead birds they put in the earth to help their vegetables grow. They found one strange big bird. It was sleeping. They decided to bring it home to the village.I think people in the village were interested in the bird. We now know that this was a Great Auk, a kind of swimming bird that lived in many parts of the North Atlantic. It was big and strong and had a loud cry. They began to talk with the other people in the village about what they should do with it. After two days, the weather got worse and then there was a terrible storm. The people in the village decided that this was because of the bird and they killed it. This was the last example of the Great Auk in Britain. Four years later, the last Great Auk in the world died in Iceland.We know the Great Auk died out because of people. But where did the people of Saint Kilda go? This is more difficult to explain. Some say that they were bored living on the island so far from modern cities. Other people think that the problem was tourists, who began to visit Saint Kilda at the end of the 19th century. A new theory says that using too many dead birds as fertilizer made their food unhealthy. I think it was a mistake to kill the auk.mistake that resulted in killing this bird. The day after the people brought the bird home, the weather got worse and worse. At that time, they had less knowledge about nature, they could only believe in God to help them decide what was wrong and what was right. So it was so easy to make mistake about what they didn"t know and it happened by mistake. The article is very clear about that. But at the first they thought they should bring it home and kill it for food. That is not good. Unfortunately, it was the last bird.”Oswaldo Herrera writes “This article is very interesting, and we need to think about it. Nowadays we are destroying our world and furthermore, the animals and plants that exist in it. There are a lot of warnings but most of the time we do not care about them - I think that this articles helps us to reflect and to be conscious about all the animals and plants that we are destroying with all the pollution that we generate nowadays.”