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奥运圣火传递来源于哪届?

2023-07-10 21:19:09
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大鱼炖火锅

1936年,奥林匹克历史上首次举行了激动人心的圣火接力仪式。火炬穿越了希腊、保加利亚、南斯拉夫、匈牙利、奥地利、捷克斯洛伐克、德国7个国家,全程3050公里,共有3331人参加了火炬接力。火炬所经过的城市都举行了隆重的欢迎仪式,并设立专门的祭坛点火。声势浩大的火炬接力活动获得极大的成功,不仅唤起了世人对奥林匹克的热情,传播了奥林匹克的精神,也为后来的奥运会树立了典范。

圣火起源于古希腊神话传说。相传古希腊神普罗米修斯为解救饥寒交迫的人类,瞒着宙斯偷取火种带到人间。古希腊在每届奥运会举行以前,人们都要在赫拉神庙前点燃圣火。现代奥运会创立后,最初并没有沿袭这个传统,直到1920年第7届安特卫普奥运会上,为了悼念第一次世界大战中死去的人们,主办者在主会场点燃了象征和平的火炬,不过既没有进行火炬传递活动,也没有从古奥林匹亚采集火种。1934年,国际奥委会决定,在奥运会期间,从开幕到闭幕,主会场要燃烧奥林匹克圣火,并且火种必须采自希腊的古奥运遗址——奥林匹亚,并以火炬接力的形式传到主办城市。从此,圣火传递成为每一届奥运会必不可少的仪式。

从1936年柏林奥运会开始,每届奥运会前,在奥林匹亚的赫拉神庙遗址前都要举行庄重的点火仪式,国际奥委会、奥运会主办地和当地的官员都要出席。身着古装的希腊少女用聚光镜采得火种,然后用火炬传到雅典,再由雅典传到主办城市。在火炬接力途中,如遇高山峻岭、江河湖海时,则可用飞机、轮船运送。火种必须在奥运会开幕前一天到达主办城市,在开幕式举行时由最后的火炬接力者在人们的欢呼声中点燃位于主体育场的“奥林匹克圣火”。有幸承担这个使命的大都是一些著名运动员。

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I have a brother and a sister. They are middle school students. I study in Xing Gong Primary School. Im in Class Two Grade Four. I have three best friends. They are Huang Yue, Xia Yu and Wang Tongtong.   We have many things in common. Do you like me? ;
2023-07-10 13:26:531

跪求一篇英语文章

AflamewaslitduringtheentireeventoftheOlympicsduringtheancientOlympicGameknownastheOlympicFlame.AnewflameislitusingaparabolicmirrortofocustheraysoftheSunattheancientOlympicStadiuminOlympia,Ellis,Greece.TheFlameisthentransportedtothehostcitywherethelastrunnerlightsthelargeOlympicCauldronbyitandthisburnsthroughouttheGames.TheFlameisextinguishedduringtheclosingceremony.TheOlympicFlamewasfirstlitduringtheopeningceremonyoftheIXOlympicGamesatAmsterdam,Netherlandsin1928.ThefirstOlympicTorchRelayoccurredatthe1936BerlinOlympicGames.圣火起源于古希腊神话传说。相传古希腊神普罗米修斯为解救饥寒交迫的人类,瞒着宙斯偷取火种带到人间。古希腊在每届奥运会举行以前,人们都要在赫拉神庙前点燃圣火。现代奥运会创立后,当圣火被点燃的那一刹那,本届奥运会圣火的传递活动即宣告圆满结束。随着奥运会的结束,本届奥运会圣火也将被熄灭。第一次火炬接力活动是从1936年柏林奥运会开始的
2023-07-10 13:27:081

《英语首字母综合填空八年级答案》 急急急~

u3002
2023-07-10 13:27:162

求一首奥运歌曲

老兄呀 我从雅典找到巴塞罗那都没找到
2023-07-10 13:27:2410

一般过去式的被动语态

回答; 一般过去时的被动语态形式是was/ were done。 如:My parents gave me a new computer as my birthday present.被动语态 →I was given a new computer as my birthday present.We were veryexcited as our team beat all the other teams in the game.被动语态 →We were veryexcited as all the other teams were beaten in the game.欢迎提问,乐意解答;愿你满意, 望你采纳。
2023-07-10 13:28:002

奥运火炬全球传递是哪年奥运会开始的?

奥运是我的目标~~~白
2023-07-10 13:28:084

奥运福娃(贝贝、晶晶、欢欢、迎迎、妮妮)的英语是什么?

Beibei Jingjing Yingying Nini
2023-07-10 13:28:167

明珠台2008年8月6号晚上9:00播的是什么电影?

欲骨查
2023-07-10 13:28:302

感悟和平的经典句子

感悟和平的经典句子你想和平,就要准备战争。韦格蒂乌斯时代呼唤战争而不是和平。 和平名言德国元首 纳粹党党魁 希特勒战争的目的必须是为了和平。古希腊哲学家 科学家 亚里士多德“正确”比“和平”更珍贵。美国第28任总统 托·伍·威尔逊我们只能用武器来保卫和平。德国元首 纳粹党党魁 希特勒没有好战争,也没有坏和平。 关于和平的名言中国好声音选手哈林庾澄庆导师队 王韵壹安得务农息战斗,普天无吏横索钱。唐·杜甫《昼梦》。息:停止。横:指态度粗暴蛮横。索:讨,征。这两句大意是:什么时候才能消除战乱,让老百姓安心种田,普天下再没有虎狼之吏横征暴敛,残酷地向老百姓搜刮钱财呢?当时社会,广大人民一方面饱受战乱之苦,一方面为苛捐杂税所逼迫。诗人每天睁开眼睛,所能看到的不是百姓躲避战乱、颠沛流离的苦况,就是百姓被敲门打户、催租逼债的惨象,只有在睡梦中,才能有片刻的安宁。因而诗人渴望着有一天能够看到天下太平,百姓安居乐业,从事正常的生产劳动,不再胆战心惊地被敲榨勒索。这两句体现了诗人的社会理想,表达丁诗人同情人民疾苦的感情。可用于表现封建社会广大人民对和平安定、宁静富足生活的向往与憧憬。唐代诗人 杜甫 《昼梦》安得壮士挽天河,尽洗甲兵长不用。唐·杜甫《洗兵马》。挽:牵引。天河:银河。甲兵:铠甲和兵器,这里泛指武器。这两句大意是:怎样能得壮士挽来天河水,彻底洗净各种武器,永远不再使用呢?诗人目睹了战乱给国家、人民带来的巨太灾难,因而希望能有一天洗净甲兵,捎灭战争。作者在这两句中驰聘想象,用浪漫主义的手挂表达了他这种兵甲不起,干戈永消的强烈愿望。可引用以抒发战乱之世人们渴望和平安定的意愿。唐代诗人 杜甫 《洗兵马》膏得锊甲作农器,一寸荒田牛得耕。唐·杜甫《蚕答行》,焉得:怎样才能得以。甲:泛指武器。选两句大意是:怎样才能把天下所有的兵器都熔化掉铸成农具。使每一寸荒地都得到开垦耕种呢?诗人痛感当时社会动乱、战争频仍给社会生产和人民生活带来的破坏,强烈希望能有一天永远消灭战争,使老百姓安居乐业,从事农桑生产。可用于表达战乱之世人民对消灭战争,恢复和平的愿望。唐代诗人 杜甫 《蚕答行》安得普天休战伐,不令竹箭困输供。 和平名言明·刘基《会稽》。竹箭:用竹子制作的箭。困:匮乏。输供:运输供应。这两句大意是:怎样才能使普天之下都停止战争,不使人们为赶制军需的竹箭而感到难以应付。两句表现了怒恨战争,渴望和平的强烈愿望,与杜甫名句“安得壮士挽天河,洗尽甲兵长不用”同一机杼。明朝开国功臣,杰出的政治家、军事家和文学家 刘基 《会稽》和平孕育着战争,战争孕育着和平。普坦汉姆只有胜利者,才能用战争去换取和平。古罗马历史学家 萨卢斯特没有和平的家庭,就没有和平的社会。日本社会活动家和宗教思想家 池田大作昨夜的暴风雨用金色的和平为今晨加冕。 关于和平的名言印度诗人,作家,哲学家,1913年诺贝尔文学奖得主 泰戈尔不能用和平取得的东西,就用拳头来取。德国元首 纳粹党党魁 希特勒和平使人民更加幸福,但也使人变得虚弱。真理才是生命之光,斗争才是和平之母……当代诗人 郭小川从来就不存在好的战争,也不存在坏的和平。 和平名言美国实业家、科学家、社会活动家、思想家和外交家 富兰克林不为战争和毁灭效劳,而为和平与谅解服务。德裔瑞士作家 海塞赢得战争只不过是使和平有一个良好的开端。英国诗人 勃朗宁为了全人类的和平与进步,中国人来到了太空。中国第一代航天员 杨利伟人类在永恒的斗争中壮大,在永恒的和平中毁灭。 关于和平的名言德国元首 纳粹党党魁 希特勒慈爱和诚实,彼此相遇。公义和平安,彼此相亲。《旧约全书》 《旧约全书》和平不是一厢情愿和单相思,更不是对合理战争的回避。军事战略专家 戴旭恶德——不和、战争、悲惨;美德——和平、幸福、和谐。英国诗人 雪莱我们不能让和平的诚意和主张,变成别人束缚我们的绳索! 和平名言军事战略专家 戴旭上天赋予的生命,就是要为人类的繁荣和平和幸福而奉献。日本松下电器创始人 松下幸之助做人要正直无欺,真实无伪,又要温厚和平,勿太梭角峭厉。清代理学家 陆陇其真理是平凡的,真理是跟平凡的事物和平凡的群众分不开的。冯定和平之音淡薄,而愁思之声要妙;欢愉之辞难工,而穷苦之言易好。 关于和平的名言唐·韩愈《荆潭唱和诗序》。要妙:亦作“要眇”,美好。工:精工,精巧。这几句大意是:歌颂升平的声音显得淡薄,而抒发愁思的诗歌常写得美妙;表现欢愉之情的作品难以写得精工,而描写穷苦困顿的作品容易写好。韩愈这几句话道出了我国文学史上一个重要的文学现象。翻开一部中国度学史,虽不乏歌功颂德、粉饰太平之作,但感人至深的还是那些感时伤世、抒发忧国忧民愁思的作品。描写愉悦之情的像杜甫《闻官军收河南河北》那样的上乘之作,实属少见,而脍炙人口、比比皆是的则是抒写穷苦与愁恨的作品。这大概与我国古代社会乱离,民生多艰,知识分子大都具有忧患意识有关。唐代文学家 韩愈 《荆潭唱和诗序》世上的暴君,若准备打一场战争,不到万事俱备,总是要侈谈和平的。奥地利作家 茨威格要进行战争只有一个借口,即通过战争我们可以生活在不受破坏的和平环境中。古罗马政治家 西塞罗闲暇当然是一种幸福。因为,我们为了获得闲暇而工作,为了获得和平而战争。古希腊哲学家 科学家 亚里斯多德全世界一切被压迫人民和被压迫民族联合起来,一切爱好和平国家要联合起来。 和平名言毛泽东理智、正义和平等都没有足够的力量统治地球上的人类,唯有利益有这种力量。第三任美国总统 托·杰弗逊你们必须跟著我庄严的宣誓:我们需要的是和平,我们需要的是献身於我们的事业。德国元首 纳粹党党魁 希特勒群众的眼睛是雪亮的。战时,雪亮的眼睛用来发现敌人;和平年代,就用来探人隐私了!赛车手、作家、时代周刊全球最具影响力100人 韩寒 《三重门》即使天才,在生下来的时候的第一声啼哭,也和平常的儿童一样,决不会就是一首好诗。关于和平的名言中国现代作家、政治评论家 鲁迅 《坟·未有天才之前》人们都追求幸福、和平,只有她是这个地球上最接近幸福的捷径,并且是谁都能得到手。瑞士作家 希尔泰爱好文艺,可使人提高品位,陶冶精神;再推而广之,帮助社会的和平,增进人生的幸福。爱的力量是和平,从不顾理性、成规和荣辱,它能使一切恐惧、震惊和痛苦在身受对化作甜蜜。英国文艺复兴时期剧作家,诗人 莎士比亚 《情女怨》一般都认为幸福存在于闲暇。不管怎么说,我们为争取闲暇而工作,为生活在和平环境而战争。 和平名言古希腊哲学家 科学家 亚里斯多德只要土地和生产资料的私有制继续存在,资产阶级和资产阶级民中的“自由和平等”就只是一种形式。列宁我们要敢于迎接合理合法的战争,新中国六十年和平稳定的发展环境,是靠连续几场自卫反击战打出来的。军事战略专家 戴旭真理是正义的侍女,自由是正义的孩儿,和平是正义的伙伴;安全化在它的步履中,胜利跟在它的裙裾后。英国海军上将 西·史密斯一个人如果没有任何阻碍,即将永远保持其满足和平庸的状态,那么他将既愚蠢又糊涂,像母牛一样地逸然自得。 关于和平的名言布朗应该努力使子女有强健的身体,使他们在爱的环境中过和平而且宁静的童年,使他们那种美好的信心尽可能地延长。法国物理学家、化学家 居里夫人处人只要个谦逊,居家只要个和平,教子只要个学好,吃穿只要个温饱,房舍家伙只要个坚实有用,冠婚丧祭只要个合理。明代文学家,思想家 吕坤我们的生命,像世界的协奏曲,由相异的因素组成——由各种各样的声调组成,美妙的和刺耳的,尖锐的和平展的,活泼的和庄严的。法国文艺复兴后期思想家 蒙田所谓混得好的,一定有不为人知的痛苦与要付出的代价;所谓混得不好的,也有属于自己的幸福和平静。就看你怎么看待它,怎么善待拥有的好。 和平名言中央电视台节目主持人、新闻评论员 白岩松体育世界是美妙的,国际奥委会是一个由204个不同种族、不同传统、不同社会制度的成员组成的大家庭,大家和平相处,因此奥运会是青春、体育与和平的盛会。前国际奥委会主席 萨马兰奇每一个伟大的事业,开头总只为少数有闯劲的人所信奉。一个人为他所信奉的事业(比如和平)而死,岂不是胜过为他所不信奉的事业(比如战争)而受折磨吗?美籍德国犹太裔,物理学家 爱因斯坦 《反战斗争》勇气就是在恐惧和狂妄之间的一种气质和平衡因素。恐惧会产生胆怯,狂妄会导致鲁莽,而勇气会使用权人们、使那些佛教徒勇敢地面对生活中不可回避的痛苦。菲力浦·劳顿·玛丽人类的进步,好像是去永无止境的梯子。登高必须要从下面拾级而起,所以亚利安人必须要遵循实际的道路,向前进行,这道路绝不是近代和平者所梦想的道路。 关于和平的名言德国元首 纳粹党党魁 希特勒对男子而言,最难过的事就是旅行途中二男一女,这样内部永远团结不了;所幸沈溪儿的相貌还不足以让男同胞自相残杀,天底下多一些这样的女孩子,男人就和平多了。赛车手、作家、时代周刊全球最具影响力100人 韩寒 《三重门》世界上没有任何欢乐不伴随忧虑,没有任何和平不连着纠纷,没有任何爱情不埋下猜疑,没有任何安宁不隐伏恐惧,没有任何满足不带有缺陷,没有任何荣誉不留下耻辱。德国作家 格里美尔斯豪森“各种知识一直是和平共存的”,“解剖学也好,文学也好,都有同样的显赫出身,同样的目的和同样的敌人……它们根本没有必要吵架,在它们之间不存在自下而上竞争。”。俄国小说家,戏剧家 契诃夫如果一个结婚后的全部生活都和平共处我们一样幸福的话,那么我算是白白浪费了三十年的时光。假如一切能从头开始,那么我将会在呀呀学语的婴儿就结婚,而不会把时间荒废在磨牙和打碎瓶瓶罐罐上。 和平名言美国作家 马克·吐温我一向反对打人,一向主张和平说理。但是,遇到不讲理的人,遇到造谣、诽谤、污蔑开国领袖,破坏中国人民团结,给日本当汉奸的人,我忍无可忍,不能再忍!宁可为此被拘留,也不能让这等汉奸放肆、猖獗!北京航空航天大学教授 韩德强虽然我现在置身在这些战士们的中间,我并不愿做一个和平的敌人;我的意思不过暂时借可怖的战争为手段,强迫被无度的纵乐所糜烂的身心得到一些合理的节制,对那开始扼止我们生命活力的障碍做一番彻底的扫除。英国文艺复兴时期剧作家,诗人 莎士比亚爱你自己要爱在最后,珍爱那些恨你的人,诚实比起腐败会给你赢得更多的好处。在你的右手里永远举着温顺的和平枝,免得嫉妒之徒说闲话。作人要公正,不要怕;你所要达到的一切目的应该是你的国家、上帝和真理所要达到的目的。英国文艺复兴时期剧作家,诗人 莎士比亚我最喜欢的是军歌和《歌唱祖国》,《歌唱祖国》里有句歌词是这样的“我们战胜了多少苦难,才得到今天的解放!我们爱和平,我们爱家乡,谁敢侵犯我们,就叫他灭亡!”,其中也没有说要打跑,就叫他灭亡,军人就应该有这种气概! 关于和平的名言军事战略专家 戴旭最初我去学习了很多新闻报道的准则,公正、客观和平衡,后来我发现,所有准则的背后都不存在一致性。不用学习技巧,我们要试图去发现技巧背后的价值观,每一种文明,每一种社会形态背后的价值观都是不一样的。爱因斯坦说过:“伦理本身也是可以推论的。”中央电视台节目主持人、记者 柴静中国人哲学讲究友好和平,什么事情妥协互相让步,互相和平共处,这个才是真正的武道,不是我打赢你就赢了,打赢你最后不一定赢的。我和你和平共处,最后全人类团结在一起,这是真正的无招胜有招。中国古人的理想就是放掉戈去耕田,中国自古以来不希望打仗,能够和平解决最好,不要武力解决。武侠小说作家 金庸懒惰的恶果只限于懒惰者本身,它容易逗人喜爱,而又不伤及旁人;因此,它就不会像危及财产安全的诈骗行为那样,普遍受到监视;也不会像骄傲那样,必然要在别人的劣势之下,去寻觅自己的满足。懒惰具有一种无声而和平的品质,它既不凭恃夸耀招来妒嫉,也不致怀有敌意而引起憎恨;因此,谁都不会忙于去谴责它或者过问其就里。英国作家 塞缪尔·约翰逊The purpose of passing the Olympic torch by way of relay was to spread the Olympic spirit and make the seeds of peace and friendship root, grow, bloom and bear fruits in more countries.用接力跑的方式传递圣火,目的在于传播奥林匹克精神,使和平和友谊的种子在更多的国家扎根、生长、开花和结果。和平名言最深最和平的快乐,就是静观土地与人世,慢慢品位出它的没与和谐。这份快乐,乍一看也许平淡无奇,事实上,它深远而悠长。在我,生命的享受就在其中了。让那永不醒觉的人自生自灭好了,如果他们抱怨,我们把耳朵塞起来。因为,他们不肯对人生对世界,对生命,有一丝一毫感激的心。我喜欢把快乐当做一种传染病,每天将它传染给我所接触的社会和人群。台湾女作家,原名陈懋平 三毛我希望天下和平,天下百姓们安居乐业,国泰民安,没有战争。上帝想了想,这难点儿,咱实话实说啊,我没那么大道性……你换一样行吗?咱商量商量别的。我一摸身上带着一张××的相片,帝哥,你看看这个,这是我师兄弟——××,长得挺寒碜的,搞不上对象,你给他变漂亮点儿吧。上帝看了说,还是说说世界和平那事儿吧……你怎么把相片撕了,我还留着避邪呢!相声和影视剧演员、电视脱口秀主持人 郭德纲我们也是误打误撞进入影视圈的。我早期小说发表后,就被导演陆续拍成电影。起初看到我小说中的人物活生生地出现在银幕上,的确是一种奇异的经历。但是我对导演的改编很不满意,比如,把“婉君”的身份由“童养媳”改成了“表妹”我和平先生都是狂热的“电影疯子”,想到“自己总比较懂得如何疼自己的孩子”,于是,就结集一些同道,成立电影公司。不过,我们的第一部电影是《月满西楼》,而不是《窗外》,《窗外》是此前另一位导演拍的。原名陈喆,台湾女作家 琼瑶金钱:可以买“床铺”;但不能买“睡眠”。可以买“珠宝”;但不能买“美丽”。可以买“纸笔”;但不能买“文思”。可以买“房屋”;但不能买“家庭”。可以买“食物”;但不能买“食欲”。可以买“娱乐”;但不能买“快乐”。可以买“谄媚”;但不能买“忠诚”。可以买“伴侣”;但不能买“朋友”。可以买“服从”;但不能买“尊敬”。可以买“权势”;但不能买“智慧”。可以买“肉体”;但不能买“爱情”。可以买“武器”但不能买“和平”。 关于和平的名言英国作家 萨克雷说起来,这真是让我最伤心和失望的事情。我和平先生都是完美主义者,总希望把一切做《窗外》中的秦汉林青霞、《哑妻》中的刘雪华、《梅花烙》中的陈德蓉马景涛、直至《还珠》中的赵薇林心如黄奕,除了票房和收视率上的巨大成功外,琼瑶影视剧就像一个巨大的造星工厂,几乎到了琼瑶点到谁谁就红的地步。难怪琼瑶自信地写到:“你们看着美丽的林青霞、刘雪华、赵薇……还有英俊的秦汉、秦祥林、苏有朋……不妨想一想,这是一条多么美丽的生命之河。我很骄傲,因为,我收集了他们的美丽,也留住了他们的青春。”原名陈喆,台湾女作家 琼瑶这个不太好说,我见过谈了几十场恋爱,有很多床伴的人,也见过感情踏实稳固,一直相依厮守的人,我觉得我说不上花心,因为我这几十年就谈了那么几次恋爱,而且27岁结婚,数据上也不算夸张,这个世界上,我最爱的女人就是我的女儿,如果一辈子只能和一个人在一起,我就和她在一起。我和我太太的感情非常稳固,但也许其他姑娘也早已如同亲人,我甚至希望她们之间能够友好互助和平共处,就是这样。其他人会爱上我,我也许会中意其他人,但没有人能改变我和我太太的感情。我很坦率地说出自己的感想,指责辱骂到的高压,我无所谓。女孩子没有任何错,要有错,也是我王八蛋而已。韩寒“婚外情”事件引起舆论轩然大波。韩寒接受周刊专访时首次作出回应。南都娱乐周刊:你自认为是否已过“花心”或者多情的人?赛车手、作家、时代周刊全球最具影响力100人 韩寒注意这个句型,看一下黑板:今年的春节联欢会突出了__ __两个字。如果是EPS出题的话,简单的题目在这句号,难一点的后面还有话。我们现在想说的是,你做题的思路应该是这样的,我们看选项:A…吉祥B…和平C…联欢D…非典E…伊拉克战争,我们可以轻松排除D和E,前三个都可以。A可以B可以C可以的令人发指,联欢会突出了联欢两个字,在逻辑上是如此的完美无懈可击,和没说一样。如果你看到这一选项,就算有面还有空格,我可以负责的告诉你,后面不用看了,直接选C。虽然前两个也可能是对的,但是ETS决不会放弃C这个选项。记住这个思路,将来上考场可以有效的节省时间。将来做题,看到A可以,B可以,C——倪萍——对了。就是这样,如果哪个选项让你想到倪萍,这肯定是对的。看完这个我感到很惊讶,想倪萍这样的人,跟ETS出题的思路是高度吻合的,如果他考不过GRE只有一个可能的原因就是词汇不过关。牛博网创始人 罗永浩江湖上的大侠客、大名人、英雄好汉,并不像传说中一样过的挺惬意。他们和平常人一样要生活、要吃饭、要玩要喝要花钱。没有收入,又怎能花?这些侠客名人又不能去偷去抢,于是有的人就开始“兼差”兼差的行业中最好的当然就是“职业杀手”在人类所有的职业中,历史最悠久最无奈的职业,就是杀手,也是男人最原始的一种职业。干杀手的钱虽然赚得很多,但大多数是悲剧人物,因为他们“出行任务”时,随时随地都会有死的可能,而且还要过着隐姓埋名的生活。有时接到的任务是刺杀自己的亲人,那时不但不能迟疑,还要连眉头都不能皱一下。杀手不但要六亲不认,而且必须冷酷无情,更要绝情,决不能有一点儿女私情,也不能有天伦之情。绝情绝义,残酷狠暴,冷血无名,这些都是干杀手的必备条件,更重要的一点是,必须无我。没有自己的时间,没有自己的利益,没有自己的恩仇,没有自己的爱恨,属于自己的一切都必须绝离。更重要的一点是,杀手这一行没有“退出”的机会,只要你一踏进,至死才方休。如果你想等捞饱了钱,然后退出那是绝对不可能的事,就算仇人不杀你,同行的人也一定会追到你,追到你完全不能说出秘密时为止——不能说出秘密的人,在这世上大概只有死人一种而已。还有一种可能,那就是别人已认为你不可能对他们构成威胁时,或许会放过你。 和平名言武侠小说家 古龙 《边城刀声》
2023-07-10 13:28:391

英语:和…比赛的短语是?

record holder 纪录保持者 cut the record 打破纪录 record breaker 打破创造者 set a new record 创造新纪录 world record 世界纪录 champion 冠军 runner-up/the 2nd place 亚军 third place/the 3rd place 季军 medalist 奖牌获得者 gold medalist 金牌获得者 silver medalist 银牌获得者 bronze medalist 铜牌获得者 last eight 取得决赛权的八名选手 final result 决赛成绩 total points 总分 placement/ranking 名次,排名 final placing 决赛名次 medal tally 奖牌榜 lost to 败给 defeat 击败 win title 夺魁 go for gold 夺取金牌 defend title 卫冕 road to 进军 victory and ceremony/awarding ceremony 颁奖仪式 The victory ceremony for … will now take place 颁奖仪式现在开始 announce results 公布成绩 The medals will be presented in descending order-1st, 2nd, 3rd. 按第一名、第二名、第三名顺序颁发奖牌。 raise national flag and play national anthem 升国旗,奏国歌 mount the rostrum 登上颁奖台 sportsmanship trophy 风格奖 fair play trophy 公平竞赛奖 cup/trophy 奖杯 prize money 奖金 prize/trophy/award 奖品 awarding/ to present prize 颁发奖品 title 称号 undertake laps of honor 绕场一周向观众致意 国际奥委会 the International Olympic Committee (IOC) 申办城市 the bidding cities 候选城市 the candidate cities 申办2008年奥运会 bid for 2008 Olympics 主办2008年奥运会 host the 2008 Olympic Games 奥林匹克精神 the Olympic ideals; the Olympic spirit 世界奥林匹克日 the International Olympic Day 环境保护 environmental protection/environmental conservation 北京四环路 the fourth ring road in Beijing 城市基础设施建设 the city"s infrastructure construction 最后的投票 make the final vote 绿色奥运 the Green Olympics 科技奥运 the Scientific Games 奥林匹克运动会 Olympics 赛跑 race 滑冰 skating 跳水 diving 游泳 swim 举重 weight lifting 足球 soccerfootball 篮球 basketball 排球 volleyball Aquatics(水上运动) Swimming 游泳 freestyle 自由泳 backstroke 仰泳 breaststroke 蛙泳 butterfly 蝶泳 individual medley 个人混合泳 freestyle relay 自由泳接力 medley relay 混合泳接力 Water polo 水球 Diving 跳水 10m platform event 十米跳台 3m springboard event 三米跳板 synchronised diving from 10 m platform 双人十米跳台 synchronised diving from 3 m springboard 双人三米跳板 Synchronised swimming 花样游泳 Archery(射箭) Individual events 个人赛 Team events 团体赛 Athletics(田径) Track 径赛 100 m, 200 m, 400 m 100米,200米,400米 800 m, 1,500 m, 5,000 m, 10,000 m 800米,1500米,5,000米,10,000米 110 m hurdles, 400 m hurdles 110米栏,400米栏 3,000 m steeplechase 3000米障碍赛 4 x 100 m relay, 4 x 400 m relay 4×100米接力,4×400米接力 Jumping 跳跃 high jump 跳高 pole vault 撑杆跳高 long jump 跳远 triple jump 三级跳远 Throwing 投掷 shot put 推铅球 discus 掷铁饼 hammer 掷链球 javelin 标枪 Decathlon 男子十项全能 Heptathlon 女子七项全能 Road events 公路赛 marathon 马拉松 walk 竞走 Ball Games(球类运动) Badminton 羽毛球 men"s singles 男子单打 women"s singles 女子单打 men"s doubles 男子双打 women"s doubles 女子双打 mixed doubles 混合双打 Baseball 棒球 Basketball 篮球 Football 足球 Handball 手球 Hockey / Field Hockey 曲棍球 Softball 垒球 Table Tennis 乒乓球 Tennis 网球 Volleyball 排球 Beach Volleyball 沙滩排球 Cycling(自行车) Road cycling 公路自行车赛 Track cycling 场地自行车赛 sprint 追逐赛 time trial 计时赛 points race 计分赛 pursuit 争先赛 Mountain bike 山地自行车赛 ============================ 奥运会Olympic Games 奥运会选拔赛Olympic Trial 国际奥委会International Olympic Committee 奥运会会歌Olympic Anthem 奥运火炬Olympic Torch 奥运会代表团Olympic Delegation 奥运村Olympic Village 组委会organization committee 开幕式opening ceremony 闭幕式closing ceremony 吉祥物mascot 颁奖台podium 奥林匹克运动会 Olympics 赛跑 race 滑冰 skating 跳水 diving 游泳 swim 举重 weight lifting 足球 soccerfootball 篮球 basketball 排球 volleyball Aquatics(水上运动) Swimming 游泳 freestyle 自由泳 backstroke 仰泳 breaststroke 蛙泳 butterfly 蝶泳 individual medley 个人混合泳 freestyle relay 自由泳接力 medley relay 混合泳接力 Water polo 水球 Diving 跳水 10m platform event 十米跳台 3m springboard event 三米跳板 synchronised diving from 10 m platform 双人十米跳台 synchronised diving from 3 m springboard 双人三米跳板 Synchronised swimming 花样游泳 Archery(射箭) Individual events 个人赛 Team events 团体赛 Athletics(田径) Track 径赛 100 m, 200 m, 400 m 100米,200米,400米 800 m, 1,500 m, 5,000 m, 10,000 m 800米,1500米,5,000米,10,000米 110 m hurdles, 400 m hurdles 110米栏,400米栏 3,000 m steeplechase 3000米障碍赛 4 x 100 m relay, 4 x 400 m relay 4×100米接力,4×400米接力 Jumping 跳跃 high jump 跳高 pole vault 撑杆跳高 long jump 跳远 triple jump 三级跳远 Throwing 投掷 shot put 推铅球 discus 掷铁饼 hammer 掷链球 javelin 标枪 Decathlon 男子十项全能 Heptathlon 女子七项全能 Road events 公路赛 marathon 马拉松 walk 竞走 Ball Games(球类运动) Badminton 羽毛球 men"s singles 男子单打 women"s singles 女子单打 men"s doubles 男子双打 women"s doubles 女子双
2023-07-10 13:28:481

something about olympic torch and torch relay (english)

指通常还是专门08奥运的?Olympic TorchOlympic torches are used, together with miners lamps, for transporting the Olympic Flame from Olympia (Greece) to the Host City, up to the Olympic stadium. (See Olympic Charter Rule 18)-------The Beijing Olympic torch relay will reach the world"s highest peak, cover the longest distance, and involve the most people in Games history, it was announced last night. The Beijing Organizing Committee for the Games of the XXIX Olympiad (BOCOG) unveiled its torch relay route and the torch - a red and silver cloud-design tube shaped like a Chinese scroll. "As the prelude to the Games, the torch relay is one of the most important ceremonies and a major means to spread and promote the Olympic spirit," BOCOG President Liu Qi said at the launching ceremony at the China Millennium Monument in Beijing. The Olympic torch will be carried across all five continents, along the ancient "Silk Road" and 135 cities, covering 137,000 kilometers over 130 days, the longest time the flame has ever been carried. The International Olympic Committee (IOC) praised the planned route. "By traveling along the "Silk Road", a symbol of ancient trade links between China and the rest of the world, crossing the five continents and going to new places, the Beijing 2008 Torch Relay will, as its theme says, be a "journey of harmony", bringing friendship and respect to people of different nationalities, races and creeds," IOC President Jacques Rogge said. "I have no doubt the Beijing 2008 Olympic Torch Relay will leave many extraordinary memories and create new dreams for people around the world." The Olympic flame will be lit in Olympia, Greece on March 25, 2008. From March 25 to 30, the torch will travel across Greece, ending at the Panathinaiko Stadium, the site of the first modern Olympic Games in 1896. After a handover ceremony in the stadium, the flame will arrive in Beijing on March 31. The world Olympic partners Coca-Cola, Samsung and Lenovo will be the sponsors for the relay. One of the highlights of this leg will be the attempt to bring the Olympic flame in May to the highest peak in the world - Mount Qomolangma. "Lifting the Olympic flame to the peak of the world is one of our commitments in the bidding, which shows our respect to the Olympics," BOCOG Executive Vice-President Jiang Xiaoyu, said. "To reach the peak of the mountain, athletes and torchbearers should make great efforts, which is in line with the Olympic spirit of "higher, stronger and faster"." BOCOG said carrying the Olympic flame to Mount Qomolangma would not affect the natural environment there. "Green Olympics is one of the three concepts of the Beijing Games, we have issued special environmental protection manual for Mount Qomolangma," Jiang said. "The passing on the mountain will be a journey of green."
2023-07-10 13:28:551

求:关于奥运会基础词汇的中英文对照,如火炬传递:torch relay

国际保龄球联盟 Federation Internationale des Quilleurs(FIQ) 世界十瓶保龄球联合会 World Tenpin Bowling Association(WTBA) 全中 strike 连续两次全中 double 连续三次全中 triple 补中 spare 失误 error 分瓶(技术球) split 死球 dead ball 犯规 foul 慢投球 slow bowling 记分 scoring the game 记分表 score sheet 一局 one game 一格 one frame 一节 one block 最高局分 high game 平均分 average 全中位 impact points
2023-07-10 13:29:032

london 2012 olympic torch relay

伦敦2012年奥运会火炬接力
2023-07-10 13:30:081

关于“绿色奥运”的英语作文

1、But we have many problems。The duty is not just for the Chinese governmental Chinese people should do their best to welcome the beginning of BEIJING OLYMPIC GAMES! 2、It"s the polluted world, a dirty world, caused by our irresponsible behaviors, that makes those lovely animals cry. Many of us are smart enough to know how terrible it is to have a world without animals and plants. But some people still persist in making the same errors and harming the environment. 3、with a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. 4、 With a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers, Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. On its journey, the flame will be seen by and inspire more human beings than any previous relay. 1、 Today is May, 28, 2007! How time flies! Very quickly 2008BEIJING is coming OLYMPIC GAMES! The friends of all over the world will come to China. Let us meet in BEIJING in 2008! But we have many problems。The duty is not just for the Chinese governmental Chinese people should do their best to welcome the beginning of BEIJING OLYMPIC GAMES! the 2008 Beijing Olympic is a chance to let all world to know china, so the Chinese government and all Chinese people hope we this games should be the best one in the history! “ONE WORLD.ONE DREAM!” this is the belief of 2008 Beijing Olympic games! It"s the game that lets us know each other! It"s also a time to make friends! That"s all, Thank you! 2、Improving Beijing"s environment for the Olympics Ladies and gentlemen: Good morning! Today, the title of my speech is Improving Beijing"s environment for the Olympics. Have you ever seen animals cry? Do you know what makes them cry? It"s the polluted world, a dirty world, caused by our irresponsible behaviors, that makes those lovely animals cry. Many of us are smart enough to know how terrible it is to have a world without animals and plants. But some people still persist in making the same errors and harming the environment. What"s worse is that people are eating wild animals. Those people don"t know, and more importantly, they don"t want to know. This kind of behavior has caused some specific problems. For example, medical experts have suggested that eating wild animals helped spread the terrible disease SARS. Now, our world is no longer clean and beautiful. Why? Because air, water, and noise pollution has become more and more serious. Noise pollution makes us talk more loudly and become angry more easily. Water pollution makes our rivers and lakes very dirty. Air pollution is the most serious kind of pollution. Both air pollution and water pollution are not only dangerous to our health, but also dangerous to animals and plants. We"re destroying our only world. A large number of trees have been cut down, so a lot of lands have been turned into deserts. Pollution can be seen everywhere! Unquestionably it is essential that we clean up our environment. Sadly, we"ve not always appreciated the importance of protecting the environment. On the streets, you can see cars producing smog. In the bathrooms, you can see students leaving the water tap on after using them. Not only the young people, but also the elderly ones throw things everywhere without thinking its wrong, even in a beautiful park. These things are taking place every day, even in modern cities like Beijing. We can"t do that any more. We must learn how to protect the environment. If everyone is aware of protecting it instead of destroying it, the world can become cleaner, more beautiful, and more peaceful. The theme of Beijing 2008 Olympic Games is "New Peking, New Olympic". It"s the target owned by every Chinese. We should try our best to improve Beijing"s environment for Olympics. Let"s begin to act! First, start with the things around you. Don"t throw rubbish onto the road. Don"t talk loudly when you are outdoors. Turn off the water tap after using it. Go to school by bike or by bus instead of by private car. Save your pocket money and contribute to environmental protection organizations. Take me as an example; I take action in order to make our only world full of happiness. I pick up rubbish and throw it into a trashcan. I collect waste paper or bottles for recycling. I plant more trees and flowers, and so on. If you do all these things just like me, it means you have done something useful to improve the environment. We"re grateful, because the emblem of the Beijing 2008 Olympic Games is a precious treasure dedicated to the Olympic Movement by the people of China. Under the guidance of the Olympic spirit, let us do a better job in all fields of our preparation and venue construction work in an efficient way. We will combine the Olympic Movement with the latest technology and advanced culture to make the Beijing Games the best Olympic Games ever! Welcome to Beijing! Thank you. 3、 China has its own sport legends. Back to Song Dynasty, People started to play a game called Cajun, which is regarded as THE origin of Ancient football. So now, you will understand why our women football team is so good today with a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. On its journey, the flame will be seen by and inspire more human beings than any previous relay. I am afraid I cannot prevent the whole picture of our cultural programs within such a short period of time. Actually, what we have shown you here today is only a fraction of Beijing that awaits you. Ladies and gentlemen, I believe that Beijing will prove to be a land of wonders to athletes, spectators and the worldwide television audience alike. Come and join us. Thank you. Thank you all.4、.With a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. On its journey, the flame will be seen by and inspire more human beings than any previous relay. I am afraid I cannot prevent the whole picture of our cultural programs within such a short period of time. Actually, what we have shown you here today is only a fraction of Beijing that awaits you. Ladies and gentlemen, I believe that Beijing will prove to be a land of wonders to athletes, spectators and the worldwide television audience alike. Come and join us. Thank you. Thank you all. 5、China has its own sport legends. Back to Song Dynasty, People started to play a game called Cajun, which is regarded as the origin of Ancient football. So now, you will understand why our women football team is so good today. With a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers, Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. On its journey, the flame will be seen by and inspire more human beings than any previous relay. I am afraid I cannot prevent the whole picture of our cultural programs within such a short period of time. Actually, what we have shown you here today is only a fraction of Beijing that awaits you. Ladies and gentlemen, I believe that Beijing will prove to be a land of wonders to athletes, spectators and the worldwide television audience alike. Come and join us. Thank you. Thank you all. 6、Environment improving for Olympics the Beijing Municipal government has issued a timetable for improving the city before the start of the 2008 Olympics. By June next year, more than three hundred illegal(违法的) buildings will be demolished(拆除). The planning of outdoor advertising boards in the city will be finished before the National Day holiday. And by the end of November, areas beside the railway and light-rail(轻轨) within the city"s fifth ring(五环) road, will be covered with trees and grass. The outside of buildings that face the street will be washed and repainted before 2008. 7、 China has its own sport legends. Back to Song Dynasty, People started to play a game called Cajun, which is regarded as the origin of Ancient football. So now, you will understand why our women football team is so good today. With a concept inspired by the famed Silk Road, our Torch Relay will break new ground, traveling from Olympia through some of the oldest civilizations known to man-Greek, Indian and Chinese. Carrying the message “Share the peace, Share the Olympics. “ The flame will pass through Tibet, cross the Yangtze and Yellow Rivers Travel the Great Wall and visit Taiwan and the 56 Ethnic communities who make up our society. On its journey, the flame will be seen by and inspire more human beings than any previous relay. I am afraid I cannot prevent the whole picture of our cultural programs within such a short period of time. Actually, what we have shown you here today is only a fraction of Beijing that awaits you. Ladies and gentlemen, I believe that Beijing will prove to be a land of wonders to athletes, spectators and the worldwide television audience alike. Come and join us. Thank you. Thank you all. 8、《New Beijing,the Three-Colored New Olympics》 Good afternoon ,ladies and gentlemen. Bidding for the Olympic Games is, in a way, an image-creating undertaking. The first and foremost thing is to let people fall in love with the city at first sight, attracting them by its unique image. What image does Beijing intend to create for itself once it has the opportunity to host the 2008 Olympics? It is known to all that the Beijing Municipal Government has already set the theme for the future games: New Beijing, Great Olympics. For me, the 2008 Olympics will be a great green Olympics illuminated with two more special colors: yellow and red. First, yellow is a meaningful color. The Yellow River is China"s mother river and the cradle of Chinese civilization. We are of the yellow race and descendants of the Yellow Emperor. This color has a special origin and great significance for the Chinese people. Beijing is the capital of New China and previously the capital for nine dynasties in Chinese history. So, yellow will naturally add splendor to the 2008 Games. Secondly, the 2008 Olympics will be a red pageant. Red is another traditionally cherished color for the whole country. We adore red. On big occasions, we like to decorate our homes in red. It is the color of double happiness, representing joyous moments, auspiciousness, enthusiasm and prosperity. Red is one of the most suitable colors to describe the future of Beijing. Beijing, together with the whole country, is becoming more and more prosperous in the process of modernization. Above all, the 2008 Olympics will be a green Olympics. Adding the green ingredient is essential in creating an appealing image, as we can"t deny the fact that Beijing, at the moment, is not as green a city as what we like it to be. Striving for an environmentally appealing city has become a central task for all the citizens of Beijing. Big efforts have been made in pollution control, replanting and beautification of the city. Certainly, all of this is no easy task. But I am sure that all of us have confidence that we will realize these green goals. For now we have the full support and participation of the environmentally conscious citizens. Each citizen is showing great concern for every one of the steps the city government takes. As the saying goes, “United, we stand,” and a green Beijing will be achieved. When our aspiration becomes reality, it will be a unique Olympics. New Beijing, Great Olympics will be weaved of these three superb colors: yellow, red and green. Let us welcome it and look forward to it! Thank you!
2023-07-10 13:30:151

Three days later,the torchbearers________the Olympic torch relay in Ningbo of Zhejiang Province..

A 试题分析:句意:三天后,火炬手在浙江省宁波市开始传递。Take up着手开始,开始(从事于);pick up捡起,好转,接人;bring up教育,培养;put up张贴,搭起。选A。
2023-07-10 13:30:221

英语中过去式的被动语态是什么构成?

一般过去时的被动语态的构成是:was/were+过去分词 u261e u2667手工翻译u2600尊重劳动u2600欢迎提问u2600感谢采纳u2667 u261c
2023-07-10 13:30:292

The Olympic flame ,torch and relay draw on a history going back to the ancient Olympic games.

奥利匹克火炬传递可以追溯到古代奥利匹克时代
2023-07-10 13:30:362

There are so many people watching the relay of the Olymoic Torch that we got____in the crowd

A.lost 是正确的
2023-07-10 13:30:432

电动牙刷美白和清洁模式什么时候用

清洁模式每天一早一晚的时候用,美白模式一周用一到两次。清洁模式:日常清洁最常用的默认模式,每天早晚2次就能保证基本的清洁美白需求,强度适中。美白&抛光模式:口腔牙菌斑和顽固牙渍的克星,尤其是经常喝奶茶喝咖啡抽烟的人,每周使用1-2次,坚持就能拥有一口大白牙。电动牙刷技术机理:1954年,瑞士医生Philippe-Guy Woog发明了电动牙刷,60年过去了,现在的电动牙刷居然与这位前辈的极为相似。从原理上分,电动牙刷有两大类:旋转与振动。旋转类的牙刷原理简单,就是电动机驱动圆形刷头旋转,在执行普通刷牙动作的同时加强摩擦效果,旋转类的牙刷噪音大,牙面清洗很干净,牙缝清洗相对较弱,但对牙齿磨损大,不建议长期使用。振动类型的牙刷更复杂,从价格上来看也更高端。振动类型的牙刷内部有一个电驱动的振动电机,能让刷头产生垂直于刷柄方向的高频摆动,但摆动的幅度很小,一般为上下各5毫米左右,行业最大的摆幅是6毫米。刷牙的时候一方面高频摆动的刷头能高效完成洗刷牙齿的动作,另一方面超过30000次每分钟的振动也让口腔里的牙膏和水的混合物产生大量微小的气泡,气泡爆裂时产生的压力可以深入牙缝清洁污垢。
2023-07-10 13:21:511

pcr试验是什么意思?

聚合酶链式反应(Polymerase Chain Reaction),简称PCR,是一种分子生物学技术,用于放大特定的DNA片段。可看作生物体外的特殊DNA复制。 DNA聚合酶(DNA polymerase I)最早于1955年发现 ,而较具有实验价值及实用性的Klenow fragment of E. Coli 则是于70年代的初期由Dr. H. Klenow 所发现,但由于此酶不耐高温,高温能使之变性, 因此不符合使用高温变性的聚合酶链式反应。现今所使用的酶(简称 Taq polymerase), 则是于1976年从 温泉中的细菌(Thermus aquaticus)分离出来的。它的特性就在于能耐高温,是一个很理想的 酶,但它被广泛运用则于80年代之后。PCR最初的原始雏形概念是类似基因修复复制,它是于1971年由 Dr. Kjell Kleppe 提出。他发表了第一个单纯且短暂性基因复制(类似PCR前两个周期反应)的实验。而现今所发展出来的PCR则于1983由 Dr. Kary B. Mullis发展出的,Dr. Mullis当年服务于PE公司,因此PE公司在PCR界有着特殊的地位。Dr. Mullis 并于1985年与 Saiki 等人正式表了第一篇相关的论文。此后,PCR的运用一日千里,相关的论文发表质量可以说是令众多其它研究方法难望其项背。随后PCR技术在生物科研和临床应用中得以广泛应用,成为分子生物学研究的最重要技术。Mullis也因此获得了1993年诺贝尔化学奖。DNA的半保留复制是生物进化和传代的重要途径。双链DNA在多种酶的作用下可以变性解链成单链,在DNA聚合酶与启动子的参与下,根据碱基互补配对原则复制成同样的两分子挎贝。在实验中发现,DNA在高温时也可以发生变性解链,当温度降低后又可以复性成为双链。因此,通过温度变化控制DNA的变性和复性,并设计引物做启动子,加入DNA聚合酶、dNTP就可以完成特定基因的体外复制。 但是,DNA聚合酶在高温时会失活,因此,每次循环都得加入新的DNA聚合酶,不仅操作烦琐,而且价格昂贵,制约了PCR技术的应用和发展。 发现耐热DNA聚合同酶--Taq酶对于PCR的应用有里程碑的意义,该酶可以耐受90℃以上的高温而不失活,不需要每个循环加酶,使PCR技术变得非常简捷、同时也大大降低了成本,PCR技术得以大量应用,并逐步应用于临床。 PCR技术的基本原理 类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物。PCR由变性--退火--延伸三个基本反应步骤构成:①模板DNA的变性:模板DNA经加热至93℃左右一定时间后,使模板DNA双链或经PCR扩增形成的双链DNA解离,使之成为单链,以便它与引物结合,为下轮反应作准备;②模板DNA与引物的退火(复性):模板DNA经加热变性成单链后,温度降至55℃左右,引物与模板DNA单链的互补序列配对结合;③引物的延伸:DNA模板--引物结合物在TaqDNA聚合酶的作用下,以dNTP为反应原料,靶序列为模板,按碱基配对与半保留复制原理,合成一条新的与模板DNA 链互补的半保留复制链重复循环变性--退火--延伸三过程,就可获得更多的“半保留复制链”,而且这种新链又可成为下次循环的模板。每完成一个循环需2~4分钟, 2~3小时就能将待扩目的基因扩增放大几百万倍。
2023-07-10 13:21:552

谁会用attack造句啊?

1. We started an attack on the fort.我们对要塞发起冲击。 2. He gave us a suprise attack.他给我们突然一击。 3. Two campers were attacked by a bear last night.两个野营者昨晚遭到一只熊的攻击? 4. Critics have attacked her ideas as antidemocratic.评论家批评她的观点是反道德的。 5. The more he speaks out,the more he comes under attack.他说的越多,受到的攻击越多。
2023-07-10 13:21:552

todayislastday中文是啥意思

今天是最后一天
2023-07-10 13:21:563

我是翠鸟,请问Wi-Fi与WiMax及WWAN的详细区别?谢谢!

简单的说,wimax就是wiman(无线城域网)的一种。是wifi的加强版。wimax覆盖范围有50平方公里。wifi只有百来米。wimax传输速率比wifi也高很多。
2023-07-10 13:21:573

excel中怎么使用函式计算某个月的最后一天日期

excel中怎么使用函式计算某个月的最后一天日期 下个月的上一天就是上个月的最后一天,例如x年y月的最后天的公式为: =DATE(X,Y+1,1)-1 excel怎么计算某个月的最后一天日期 可以利用日期相加 自动识别的功能。 比如A1为某天日期,A2为要求的最后一天,在A2输入 =DATE(YEAR(A1),MONTH(A1)+1,DAY(0)) 如何使用excel计算出某月份的最后一天日期 用下个月的1号,减1,可以得到本月的最后一天的日期。 用SQL获取某个某年某个月份的最后一天的日期 /* 思路:首先把某日期的月份加一,再把结果的日期减一 */ DECLARE @Date AS DATETIME DECLARE @DateChar AS nVarChar(19) --给日期赋值 SET @Date = GetDate() --返回你需要的 SET @DateChar = CONVERT(Char(8), @Date, 120) + "01 00:00:00" SELECT DATEADD(d, -1, DATEADD(m, 1, CAST(@DateChar AS DATETIME))) java中如何获取某个日期是当月的最后一天 import java.util.Calendar; import java.util.Date; public class Test { public static void main(String args[]) { System.out.println(getLastDayOfMonth(new Date())); } public static Date getLastDayOfMonth(Date sDate1) { Calendar cDay1 = Calendar.getInstance(); cDay1.setTime(sDate1); final int lastDay = cDay1.getActualMaximum(Calendar.DAY_OF_MONTH); Date lastDate = cDay1.getTime(); lastDate.setDate(lastDay); return lastDate; } } 使用SQL Server日期函式计算某一天是周几 今天是星期几,例子 1: set language N"English" select datename(weekday, getdate())Wednesday 今天是星期几,例子 2: set language N"Simplified Chinese" select datename(weekday, getdate())星期三 今天是星期几,例子 3: set datefirst 1 select datepart(weekday, getdate()) 3 -- 星期三 注意:这里用到了另外一个 SQL Server 日期函式 datepart()。 set datefirst 1 表示设定一周的第一天为星期一。在老美原始码天空 ,他们的一周的第一天习惯为星期天。我们可以通过 @@datefirst 函式得到当前会话的 datefirst 设定值。 select @@datefirst1 你可能有疑问:set language 后面的语言引数是从哪里得到的?可以得到其他国家语言中的星期几吗?当然是可以的了, 请看下面: vb 如何找出某个月的最后一天 Dim sDateVal As String sDateVal = "2013-06-24" MsgBox DateAdd("D", -1, DateAdd("M", 1, Format(sDateVal, "YYYY-MM-" & "01"))) Java种如何获取某个日期是当月的最后一天 说明一下,日期类中的月份是0-11比这际月份少1. 这样你调置 2010 2 这其实是 2010 年 3月份。这个月分的0天是那一天呢,就是 3月1号减一天 即上个月的最后一天。 import java.util.Calendar; public class Test { public static void main(String[] args) { System.out.println(daysCount(2010, 2)); } public static int daysCount(int year, int month) { Calendar cal = Calendar.getInstance(); cal.set(Calendar.YEAR, year); cal.set(Calendar.MONTH, month); cal.set(Calendar.DATE, 0); return cal.get(Calendar.DATE); } }
2023-07-10 13:22:021

乔布斯发明的东西是什么?U0001f62e

苹果,他是电脑公程师
2023-07-10 13:21:5015

LAST DAY &请食野

更新1: for around 72 portion ,最好自己做甜品啦, 做你识做甚么甜品, 我有个同事请我食杨支甘露+芒果布甸, 做大菜糕、千层糕、糯米滋都是唔错选择, 如果易做既,可选择啫喱糖和搾果汁, 又如果唔想做,买曲奇饼、西饼或蛋挞都已经几好了. 图片参考:.yimg/iugc/rte/ *** iley_32. , 你好, 我同意005网友既提意, 你负责茶水呢部份, 但可以简单D, 你可以买几款纸盒饮品放响公司雪柜 (6盒装, 14 pack), 庆祝之时放出黎, 就可以啦..... (会比较花算一D)。 因为如果任佢地去餐厅叫饮品, 成70几杯, 都几系野架。 谢谢。 ,已经有人请食Pappagallo ice-cream, fruit salad 同西饼,咁你就应该负责出茶水钱就得嘞,始终都系下午茶时间,出钱叫嘢饮最正路。或者你应该和其他一起请客嘅同事商量,可以一起夹钱,咁可以避免提供重复食物,又唔会出现某人提供的较受欢迎,某人提供的无人吼嘅场面。,你可以买包装的朱古力 OR 任何CANDY (最紧要好味) 例如:雪吻,雪士莲...等 其实,你工资又不多,你亦无需要太过破费...只要有心就可以喇!!! 如果,你想留给一个好印象,可以将糖果/朱古力用1至2糖为一份,包装给每一位同事都可以...,西饼就ok啦...因为我地通常称呢d为散水饼...何况你人工只有3000蚊...我谂佢地都睇得开既...唔好甘担心...因为呢d请野食只不过系心意.如果唔系好多人或者你可以考虑下泡芙啦...都几特别..都系十元左右一个...,
2023-07-10 13:21:481

E基因_人HTRIE基因在不同细胞系和多种组织中的表达及其功能

  摘 要:人的HTRIE基因是五羟色胺受体(HTR)家族中的一员,这个家族里面的基因都和精神分裂症、抑郁症以及人的自杀活动有关,但是对于其具体的作用机制目前研究不多,采用实时定量PCR方法分析了HTRIE基因在各个组织和不同细胞系中的表达情况,亚细胞定位发现HTRIE位于细胞膜上,萤光素酶实验分析确定HTRIE可以抑制原癌基因erbB2启动子的转录活性,这些研究结果为进一步研究HTRIE基因在疾病发生中的作用奠定了一定的基础。   关键词:HTRIE;表达谱;实时定量PCR:转录活性   中图分类号:Q7 文献标识码:A 文章编号:1007-7847(2007)04-0311-05      人类的五羟色胺受体可以分为7种类型,即5-HTRI~7,这7种类型又可以分为14种亚型,分别为:HTR1A,HTR1B,HRTIC,HTRID,HTRIE,HTI1F,HTR2A,HTR2B,HTR2C,HTR3A,HTR4,HTR5A,HTR6,HTR7,有研芋云表明HTR7和HTR4都可以激活转录因子固醇反应元件(serumresponse element,SRE)的转录活性,在NIE-115细胞系中过表达5-HT4(a)可以使其变圆,在海马神经元中,HTR7有使神经轴突变长的功能,HTRlB能够和S100A10,Pll(annexin 2 lightchain)蛋白质发生相互作用,而且发现HTRlB对下游ERK1/2的Thr202/TYr204磷酸化具有调控作用,而且可以通过和pll相互作用抑制神经冲动的传导,人HTRIE基因位于染色体的6q14-q15,并且含有7个跨膜结构域,因此属于G-蛋白偶联受体家族,目前发现此蛋白质在背根神经中枢有表达,并且具有抑制cAMP的形成,而且在去甲肾上腺素、肾上腺素、组氨、多巴胺和褪黑激素的刺激下,其蛋白质的表达情况没有任何变化,研究发现在阻断剂methiothepin的刺激下,HTRlE抑制cAMP的形成作用消失,有研究表明,五羟色胺诱导钙离子释放是通过激活HTR1E来实现的,而且发现HTtR1E可以调节细胞因子IL-6和CXCL8/IL8的释放,因此HTR1E可能在激活各种细胞信号途径以及调节细胞因子的释放起到了很重要的作用,我们通过对HTRIE基因在各个组织和细胞系中的表达情况以及亚细胞定位分析,为以后对HTRIE功能的研究打下基础。      1 材料与方法      1.1 材料   真核表达载体pEGFP-C3和含有erb B2启动子的报告基因pTAL-luc-erbB2购自Clontech公司,pMDl8-T载体、不含erbB2启动子区域的报告基因的载体pTAL-luc、各种限制性内切酶、T4DNA连接酶、T4聚合酶及反转录试剂盒购自TaKaRa,LipofectamineTM 2000转染试剂盒,总RNA提取的TRIZOL试剂购自Invitrogen,luciferase assay试剂盒购自Promega,SYBRGreen I荧光染料实时定量PCR试剂盒购自Applied Bio systems公司,倒置荧光显微镜为Zeiss产品,PE全自动荧光定量PCR仪为美国ABI 7900产品,荧光分光光度计TD-20/20为美国产品,T47D、HeLa、HepG2、Hep3B、SW480、MCF7等14种细胞系以及人的各种组织由实验室保存。      1.2 方法   1.2.1 细胞培养、总RNA的提取、cDNA的合成T47D、HeLa、HepG2、Hep3B、SW480、MCF7等细胞在37℃,5%CO2,相对湿度95%条件下,用完全培养液DMEM(补加10%新生牛血清、2mol/L谷氨酰胺和100mg/L青霉素和100mg/L链霉素)培养,24h以后用PBS将细胞洗两次,然后用细胞刮收集细胞,各种细胞系和组织按照Invitrogen公司试剂盒提供的方法进行,用反转录试剂盒合成各细胞系和组织的cDNA片段。   1.2.2 目的基因的扩增以及重组载体的构建   从人大脑组织中提取RNA,然后以反转录的cDNA为模板,以HTRIE引物对(sense primer:5′-CTCGAGATGAACATCACAAACTGTACCAC-3′,添加Xho I位点anti-sense primer 5′-GGTACCCTAAGTATGCTCTCGGCATCT-3′,添加gpn I)克隆人HTRlE编码序列(1098bp),然后亚克隆到pMDl8-T载体中,用Xho I/Kpn I酶切鉴定正确后,用Xho I/Kpn I酶切将目的片段切下,再亚克隆到同样酶切处理的pEGFP-C3表达载体中,酶切验证后测序分析,以确保读码框和序列的正确性。   1.2.3 实时定量PCR   用Trizol抽提各种细胞系的细胞总RNA,取总RNA 2μg用SuperscriptⅡ逆转录试剂盒进行逆转录反应,再以逆转录反应液2μL作为模板,对TNFAIP l基因的表达采用实时荧光定量PCR检测,定量引物采用Primer Express 3.0软件设计(sense primer:5′-TCCACCAG CCTGCCAACTAC-3′anti-sense primer 5′-CCGTCCTCq"TCCTGGCGTAT-3′);反应体系为:1×SYBR Green PCRMaster Mix,50mmol/L sense primer,50 mmol/Lanti-sense primer,100ng模板进行,反应条件为:95℃变性15s、60℃复性60s,72℃延伸60s,共40个循环,以β-actin作为内参照,用去离子水取代模板作阴性对照,用美国PE Biosystems公司提供的SDS2软件进行数据处理,以RO值为纵坐标做直方图,比较表达差异,每个样品重复3次。   1.2.4 HTRIE亚细胞定位   用完全培养液DMEM培养Hep3B细胞长到70%~80%时,将pEGFP-C3-HTRIE转染到Hep3B细胞,细胞培养24h后,用PBS洗细胞一次,然后用4%的多聚甲醛室温固定10min,PBS洗两次,随后将50%的丙酮和50%甲醇混合液加入到细胞中室温放置30s后,PBS洗两次,然后加入终浓度为0.5mg/L的Hochest染色液,室温放置30min,用PBS洗两次后在荧光显微镜下观察细胞。   1.2.5 萤光素酶实验检测HTR1E对erbB2转录活性的影响   用完全培养基DMEM培养HEK293Fr细胞,等到细胞长到70%~80%时,将pTAL-luc-erbB2与pEGFP-C3-HTRIZ,pTAL-luc与pEGFP-C3-HTRIE,pEGFP-C3与pTAL-luc-erbB2,pTAL- luc与pEGFP-C3分别共转染到培养好的HEK293Fr细胞中,每组重复3次,每孔用LacZ共转染到细胞中来平衡用量,转染24h后,将细胞用PBS洗两次,裂解细胞抽提蛋白,测定各组分蛋白的荧光强度。      2 结果      2.1 细胞和组织的总RNA的提取及构建真核表达载体的成功   将细胞和组织中提取的RNA各取2μL进行琼脂糖电泳,可以观察到清晰的28S、18S、5S条带,RNA的提取效果理想,然后检测各RNA的A260/A280值,其A260/A280值都在1.85左右,说明提取的RNA有着较高的浓度(图1),反转录后,克隆的人HTRIE基因片段在约1000bp处获得单一目的条带,与克隆基因1098bp的大小基本一致(图2),克隆基因亚克隆到真核表达载体pEGPF-C3中,Xho I/xpn I酶切验证得到一条约1000 bp的条带(图3),测序结果进一步说明HTRIE基因亚克隆到表达载体中,而且读码框正确。      2.2 HTRIE在各个组织和细胞系中表达分析   用RT-PCR检测HTR1E在常见的细胞系和组织中的表达情况(图4),结果显示HTRIE基因在细胞中的表达情况为:人的乳腺癌细胞T47D和纤维素瘤细胞HTl080-Tetoff表达量最低,而在人的肝癌细胞HepG2表达量最高,在所有检测的组织中都有较高的表达,但是在肝脏组织表达量最高,在脾脏组织表达量最低,在实验过程中,目的基因和内参β-actin在琼脂糖电泳后都只有单一的一条带,说明两个基因都是特异性的扩增,实验没有污染(结果没有显示)。      2.3 HTRIE定位于细胞膜上   在Hep3B细胞中转染pEGFP-C3-HTR1E,经过Hocheset染色后,然后在荧光显微镜下观察细胞核和目的基因的分布情况,结果发现HTR1E定位于细胞膜上(图5)。          2.4 HTRIE可以抑制原癌基因erbB2的转录活性   将p7AL-luc-erbB2与pEGFP-C3-HTRIE,pTAL-luc与pEGFP-C3-HTRIE,空载体pEGFP-C3与pTAL-luc-erbB2,pTAL-luc与空载体pEGFP-C3分别共转染到HEK293FT细胞中,每组重复3次,转染24h后,测定报告基因的荧光值,结果发现在HEK293Fr细胞中过表达H7R1E后,报告基因erbB2的荧光值明显下降,没有erbB2启动子的报告基因的pTAL-luc在过表达H7R1E后荧光值没有明显的下降的趋势,这说明HTRlE对原癌基因erbB2的转录有抑制作用(图6)。      3 讨论      原癌基因ERBb2是表皮生长因子受体(EGFR)家族中的一员,研究发现,在小鼠中敲除erbB2基因11d后小鼠死去,并且其运动神经和颅神经嵴衍生感官神经节的发育也受到了严重的影响EGFR(erbBl),erbB3和erbB4 3个基因都需要与配体的结合才能够被激活,但是erbB2的激活并不需要与配体的结合,这意味着其他的信号分子也可以通过erbB2来传递信号,目前对与erbB2致癌的研究主要集中在细胞水平,并已经证实了erbB2可以通过G-蛋白偶联受体的信号通路来激活,在许多类型癌症中erbB2都出现过表达的情况,所以在治疗癌症时,erbB2也成为药物治疗的靶标,我们通过对HTREI的亚细胞定位发现,作为G-蛋白偶联受体的HTRIE定位于细胞膜上,而且发现此基因在各个组织和细胞中都有表达,并可以抑制erbB2的启动子的转录活性,这也就意味着HTRIE基因可能在癌症的发生中扮演了重要角色,为以后HTRIE功能的研究打下了基础。      作者简介:吴艳阳(1981―),男,湖南张家界人,硕士研究生,主要从事转录因子调控机理的研究;张健(1963―),男,湖南长沙县人,湖南师范大学教授,通讯作者,主要从事转录因子调控机理的研究。
2023-07-10 13:21:461

hit,attack,turn on的区别

【hit】一般是碰撞,袭击,碰撞,打击等。而且如果说,打某人后面必须接具体的身体部位,比如说,打他的脸——hit him on the face【attack 】是常用词,指"攻击敌人"或"用言论攻击他人"具体的意思有:①攻击②非难,抨击; 辱骂③着手,投入,从事④(疾病)侵袭⑤腐蚀,侵蚀【turn on 】指的是打开的意思,没有必要和hit放一起作比较,有关turn的短语很多,你可以放再一起熟记。这边帮你列举一些希望对你有帮助in turn 轮流,依次 turn off 关掉,关闭;拐弯,使转变方向 turn into v. 变成;进入 turn on 打开,发动 turn out 生产;结果是;关掉;出动;驱逐 turn over 移交给;翻阅;把…翻过来;发动;营业额达到;反复考虑 turn left v. 向左转 turn right v. 向右转 turn around 船只留港 turn in 交上;归还;拐入;告发;[口]上床睡觉 turn back 往回走;阻挡;翻回到 turn up 出现;发生;开大;发现;卷起;使仰卧 turn out to be 结果是;原来是 turn down 拒绝;向下转折 turn from 对…感到厌恶 your turn 轮到你;该你了 turn away 避开;解雇;不准…入内;走开;转过脸 turn round 转身,转向;归航;回车道;转变 turn of the century 世纪之交;19世纪与20世纪转换交替时期;19世纪末20世纪初 by turns 轮流;交替
2023-07-10 13:21:461

win10怎么激活?win10激活工具怎么用

1、将下载后的解压文件进行解压,就会发现文件夹中只包含一个可执行文件“Win10激活工具.exe”或“ome8.exe”程序,双击即可运行。2、在打开的“Win10一键激活工具”主界面,点击“一键永久激活Windows和Office”按钮。3、如果激活成功,则显示“Windows10激活成功”或“Office 2013 ProPlus激活成功“的提示。4、此外,我们也可以使用“KMS激活工具”来激活Win10正式版及预览版操作系统。
2023-07-10 13:21:432